[1]李采青,韩旭颖,曹 静,等.泌尿系感染常见细菌对尿液磷酸铵镁结晶的影响[J].现代检验医学杂志,2017,32(02):131-134.[doi:10.3969/j.issn.1671-7414.2017.02.036]
　LI Cai-qing,HAN Xu-ying,CAO Jing,et al.Study Formation of Ammonion-Magnesium Phosphate Crystals in Urine with Bacteria Growing[J].Journal of Modern Laboratory Medicine,2017,32(02):131-134.[doi:10.3969/j.issn.1671-7414.2017.02.036]
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泌尿系感染常见细菌对尿液磷酸铵镁结晶的影响()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年02期
页码:
131-134
栏目:
研究简报·实验技术
出版日期:
2017-03-25

文章信息/Info

Title:
Study Formation of Ammonion-Magnesium Phosphate Crystals in Urine with Bacteria Growing
文章编号:
1671-7414(2017)02-131-04
作者:
李采青; 韩旭颖; 曹 静; 张 伟; 李玉芬; 刘金禄
河北北方学院附属第一医院微生物科,河北张家口 075000
Author(s):
LI Cai-qing; HAN Xu-ying; CAO Jing; ZHANG Wei; LI Yu-fen; LIU Jin-lu
Microbiological Laboratory, the First Affiliated Hospital,Hebei Northern University,Hebei Zhangjiakou 075000,China
关键词:
磷酸铵镁结晶;  细菌;  培养;  尿液
分类号:
R691.3; R446.12
DOI:
10.3969/j.issn.1671-7414.2017.02.036
文献标志码:
A
摘要:
目的 了解泌尿系感染常见细菌对尿液中磷酸铵镁结晶形成的影响,为临床积极抗感染治疗,防止结石形成提供依据。方法 将大肠埃希菌、奇异变形杆菌、铜绿假单胞菌、肺炎克雷伯菌、肠球菌五种细菌分别配成0.5McFarland,取10 μl菌悬液于2 ml新鲜无细菌及其他有形成分的尿液中,置35℃孵箱培养,24 h和48 h后用超高倍显微镜对培养液进行观察和对磷酸铵镁结晶计数。25 μl菌悬液于5 ml新鲜无细菌及其他有形成分的尿液重复进行试验。结果 从无磷酸铵镁结晶培养液观察到磷酸铵镁结晶,男性形成率高于女性。奇异变形杆菌培养液中磷酸铵镁结晶形成最多,铜绿假单胞菌次之,肺炎克雷伯菌第三,肠球菌在2 ml培养液有1例形成,大肠埃希菌在5 ml的培养液中有2例形成。形成的结晶以未成形羽毛状结晶最多,立方形和方柱状次之,洋信封状结晶最少。结论 具有尿素酶细菌在尿液中繁殖时容易促进磷酸铵镁结晶形成,奇异变形杆菌是促进磷酸铵镁结晶形成的重要病原菌之一。
Abstract:
Objective To study formation of ammonion-magnesium phosphate crystals in urine with bacteria growing and provide guidance forcilinical prevention of urinary calculi.Methods Bacterial culturefluid of Escherichia coli,Proteus mirabilis,Pseudomonas aeruginosa,Klevsiella pneumoniae,Enterococcus in urine was examined directly under theultrahigh sensitive microscpcope system for ammonion-magnesium phosphate crystasl.The number of ammonion-magnesium phosphate crystasl was measured when the24th and the 48th hour.Results Ammonion-magnesium phosphate crystasl were observed from the culture fluid without ammonion-magnesium phosphate crystasl.The rate of male formation was higher than that of female.Ammonion-magnesium phosphate crystals in culture fluid of Proteus mirabilis was the highest,Pseudomonas aeruginosa was the second,the third was Klebsiella pneumoniae,and there was formed 1 case in 2 ml culturefluid of enterococcus,and 2 cases of formation in 5 ml culturefluid of Escherichia coli.Thecrystals formed were the most unformed feather crystals,followed by cubic andsquare cylinders,and the envelope-like crystals were the least.Conclusion Bacteria with urease play a significant role in ammonion-magnesium phosphate crystasl formed,Proteus mirabilis is the main pathlogen.

参考文献/References:

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备注/Memo

备注/Memo:

作者简介:李采青(1969-),女,大学本科,副主任技师,主要从事病原微生物学研究,Tel:15933138532,E-mail:lcqyffs@163.com。
更新日期/Last Update: 2017-04-10