[1]郭晓波,杨 乐,闫抗抗,等.骨髓增殖性肿瘤患者外周血单个核细胞JAK2-V617F基因突变与血栓事件发生相关性分析[J].现代检验医学杂志,2017,32(02):143-145.[doi:10.3969/j.issn.1671-7414.2017.02.040]
　GUO Xiao-bo,YANG Le,YAN Kang-kang,et al.Relationship between JAK2-V617F Gene Mutation in Peripheral Blood Mononuclear Cells and Thrombotic Events in Patients with Myeloproliferative Neoplasms[J].Journal of Modern Laboratory Medicine,2017,32(02):143-145.[doi:10.3969/j.issn.1671-7414.2017.02.040]
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骨髓增殖性肿瘤患者外周血单个核细胞JAK2-V617F基因突变与血栓事件发生相关性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年02期
页码:
143-145
栏目:
检验与临床
出版日期:
2017-03-25

文章信息/Info

Title:
Relationship between JAK2-V617F Gene Mutation in Peripheral Blood Mononuclear Cells and Thrombotic Events in Patients with Myeloproliferative Neoplasms
文章编号:
1671-7414(2017)02-143-03
作者:
郭晓波1; 杨 乐1; 闫抗抗1; 杨瑞利2; 蔺 京1
1.西安市中心医院,西安 710003;
2.西安长安医院检验科,西安 710016
Author(s):
GUO Xiao-bo1; YANG Le1; YAN Kang-kang1; YANG Rui-li2; LIN Jing1
1.Xi'an Central Hospital,Xi'an 710003,China;
2.Department of Clinical Laboratory,Chang'an Hospital in Xi'an,Xi'an710016,China
关键词:
JAK2-V617F;  骨髓增殖性肿瘤(MPN);  基因
分类号:
R733.3; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.02.040
文献标志码:
A
摘要:
目的 研究骨髓增殖性肿瘤(MPN)患者外周血单个核细胞JAK2-V617F基因突变与血栓事件发生的相关性。方法 对391例MPN患者进行血栓事件调查,同时采用实时荧光定量聚合酶链反应(QRT-PCR)法对患者外周血进行JAK2-V617F检测分析。结果 391例MPN患者中发现105例存在血栓性事件(发生率26.9%),273例JAK2-V617F阳性患者中84例发生血栓事件(30.8%),118例JAK2-V617F阴性患者21例发生血栓事件(17.8%),组间差异有统计学意义(χ2=32.30,P<0.01)。真性红细胞增多症(PV)组中,JAK2-V617F阳性患者发生血栓事件45例,发生率为29.2%(45/154),阴性患者发生血栓事件6例,发生率为42.9%(6/14),组间差异无统计学意义(χ2=2.13,P>0.05); 原发性血小板增多症(ET)组中,JAK2-V617F阳性患者发生血栓事件18例,发生率为32.2%(18/56),阴性患者发生血栓事件9例,发生率为15.8%(9/57),组间差异有统计学意义(χ2=12.32,P<0.01); 原发性骨髓纤维化(PMF)组中,JAK2-V617F阳性患者发生血栓事件21例,发生率为33.3%(21/63),阴性患者发生血栓事件6例,发生率为12.8%(6/47),组间差异有统计学意义(χ2=14.32,P<0.01)。结论 MPN患者血栓事件与JAK2-V617F之间存在相关性,JAK2-V617F阳性的MPN患者更易发生血栓事件,尤其是ET和PMF患者。
Abstract:
Objective To analyze the correlation between mutation in JAK2-V617F gene in peripheral blood mononuclear cells and thromboticevents in patients with myeloproliferative.Methods Investigation of thrombosis in 391 patients with MPN,using QRT-PCR to detect JAK2-V617F gene mutation in peripheral blood of patients.Result 105 cases of thrombotic events in 391 MPN patients(26.9%),84 cases of thrombotic events in 273 JAK2-V617F gene mutation positive patients(30.8%),however,21 cases of thrombotic events in 118 JAK2-V617F gene mutation negative patients(17.8%).The difference between the two groups was statistically significant(χ2=32.30,P<0.01).In the PV group,45 cases of thrombosis events in JAK2-V617Fpositive patients(29.2%),6 cases of thrombosis events in JAK2-V617F negativepatients(42.9%),there was significant difference betweenthe two groups(χ2=2.13,P>0.05).In the ET group,18 cases of thrombosis events in JAK2-V617F positive patients(32.2%),9 cases of thrombosisevents in JAK2-V617F negative patients(15.8%),there was significant difference between the two groups(χ2=12.32,P<0.01).In the PMF group,21 cases of thrombosis events in JAK2-V617F positive patients(33.3%),6 cases of thrombosis events in JAK2-V617F negativepatients(12.8%),there was significant difference between the two groups(χ2=14.32,P<0.01).Conclusion JAK2-V617F positive MPN patients were moresusceptible to thrombotic events especially in patients with ET and PMF.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金青年科学基金项目资助(71603199)。
作者简介:郭晓波(1981-),男,硕士,主管检验师,主要研究方向:血液病的实验室诊断,E-mail:guoxiaobo.2007@163.com。
通讯作者:蔺 京(1980-),女,本科,主管护师,主要研究方向:临床护理,E-mail:258332579@qq.com。
更新日期/Last Update: 2017-04-10