[1]李笑峰a,王 璐,李 光b,等.慢性髓性白血病患者血清3-氨茴酰丙氨酸与尿酸水平检测的临床意义[J].现代检验医学杂志,2017,32(03):22-25.[doi:10.3969/j.issn.1671-7414.2017.03.006]
 LI Xiao-fenga,WANG Lu,LI Guangb.Clinical Significance of Serum Kynurenine and Uric Acid Levels Detection in Chronic Myeloid Leukemia Patients[J].Journal of Modern Laboratory Medicine,2017,32(03):22-25.[doi:10.3969/j.issn.1671-7414.2017.03.006]
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慢性髓性白血病患者血清3-氨茴酰丙氨酸与尿酸水平检测的临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年03期
页码:
22-25
栏目:
论著
出版日期:
2017-05-25

文章信息/Info

Title:
Clinical Significance of Serum Kynurenine and Uric Acid Levels Detection in Chronic Myeloid Leukemia Patients
文章编号:
1671-741 4(2017)03-022-04
作者:
李笑峰1a王 璐2李 光1b
1.西安市中心医院a.检验科;
b.西安市血液病研究所,西安 710003;
2.西安市儿童医院检验科,西安 710003
Author(s):
LI Xiao-feng1aWANG Lu2LI Guang1b
1a.Department of Clinical Laboratory;
1b.Xi’an Institute of Hematology,Xi’an Central Hospital,Xi’an 71 0003,China;
2.Department of Clinical Laboratory,Xi’an Children’s Hospital,Xi’an 710003,China
关键词:
3-氨茴酰丙氨酸尿酸3-氨茴酰丙氨酸/色氨酸指数色氨 酸代谢慢性髓性白血病
分类号:
R551;R446.112
DOI:
10.3969/j.issn.1671-7414.2017.03.006
文献标志码:
A
摘要:
目的 探讨慢性髓性白血病(CML)患者血清3-氨茴酰丙氨酸 (KYN)与尿酸(UA)表达的临床意义。方法 选取2014年3月~2015年12 月收治的30例慢性髓性白血病患者(CML组)和25例健康体检者(对照组)。高效液相色谱法测 定CML组和对照组血清中KYN和色氨酸(TRP),计算KYN/TRP比值(KTI);全自动血细胞分析仪 检测白细胞数;酶法检测血清尿酸;Pearson相关性分析UA与KTI和白细胞数相关性。〖HT5 ”H〗结果 治疗前CML组血清KTI(49.76±19.06)显著高于对照组(27.87±5 .06,t=4.470,P=0.001)。ROC分析KTI cutoff值为40。CML组KTI与白细胞数相关( r=0.743,P=0.001),与年龄无关(r=0.205,P=0.276);10例KTI≥40的CML组 患者治疗前KTI(64.7±17.8)和白细胞数[(96.7±64.1)×109/L]显著高于治疗后KT I(27.0±10.9,t=7.102,P=0.001)和白细胞数[(10.1±5.2)×109/L,t= 4.285,P=0.002];治疗前CML组KTI<40CML组患者(n=15)UA(354.98±103.9 μm ol/L)显著低于KTI≥40 CML组患者(n=15)UA(484.98±132.1 μmol/L,t=1.432, P=0.045)。CML组UA与KTI(r=0.573,P=0.001)及白细胞数(r=0.537,P=0.0 02)相关。结论 CML患者KTI增加提示吲哚胺-2,3-双加氧酶(IDO) 活性提高,IDO活性可能与CML发病和疗效相关,监测KTI可用于诊断CML和评价CML疗效。
Abstract:
Objective To investigate the clinical significa nce of kynurenine (KYN) and uric acid (UA) levels in chronic myeloid leukemia pa tients (CML).Methods 30 CML patients (CML group) and 25 healthy controls (Control group) were enrolled in the hospital from Mar 2014 to Dec 2015.Serum KYN and tryptophan (TRP) of all subjects were determined by high performance liquid chromatography,and KYN/TRP ratio (KTI) were calculated.Leu kocytes were counted by automatic blood cell analyzer.Serum UA were detected by enzyme method.Correlation between UA and KTI,leukocytes were analyzed by Pear son correlation.Results The serum KTI of the CML group ( 49.76±19.06) before treatment was significantly higher than that of the contr ol group (27.87±5.06,t=4.470,P=0.001).KTI cutoff value was 40 by ROC analysis.There had correlation between KTI and leukocytes (r=0.743,P=0. 001) and no correlation between KTI and ages (r=0.205,P=0.276).KTI (64. 7±17.8) and the leukocytes [(96.7±64.1)×109/L] of 10 cases of KTI≥40 of CML patients before treatment was significantly higher than that of KTI (27 .0±10.9,t=7.102,P=0.001) and the leukocytes[(10.1±5.2)×109/L, t=4.285,P=0.002] after treatment.The serum UA in KTI<40 CML patients ( n=15, 354.98±103.9 μmol/L) was significantly lower than that of KTI≥40 CML group CML (n=15, 484.98±132.1 μmol/L,t=1.432,P=0.045).The UA in CML group was associated with KTI (r=0.573,P=0.001) and leukocytes (r=0.537,P=0.002).Conclusion The increase of KTI in CML patients suggested that an increased activity of indoleamine-2,3-diox ygenase (IDO).IDO activity may associated with CML morbidity and therapy effect .The detection of KTI may contribute to the diagnosis of CML and assessing ther apy effect of CML.

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基金项目2013JM4016。
作者简介:李笑峰(1983-),男,本科,主管检验技师 ,研究方向:生化、免疫、检验,E-mail:lixiaofeng1983919@126. com。
通讯作者:王 璐,主管检验技师,E-mail:71900104@qq.co m。
更新日期/Last Update: 2017-06-25