[1]刘 云,万玉香,马 炜,等.VITEK 2 Compact检测耐碳青霉烯类肺炎克雷伯菌对阿米卡星药敏结果的准确性评价[J].现代检验医学杂志,2018,33(01):133-136.[doi:10.3969/j.issn.1671-7414.2018.01.001]
 LIU Yun,WAN Yu-xiang,MA Wei,et al.Evaluation on the Accuracy of VITEK 2 Compact for the Susceptibility of Carbapenem-Resistant Klebsiella Peumoniae to Amikacin[J].Journal of Modern Laboratory Medicine,2018,33(01):133-136.[doi:10.3969/j.issn.1671-7414.2018.01.001]
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VITEK 2 Compact检测耐碳青霉烯类肺炎克雷伯菌对阿米卡星药敏结果的准确性评价()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年01期
页码:
133-136
栏目:
研究简报·实验技术
出版日期:
2018-02-05

文章信息/Info

Title:
Evaluation on the Accuracy of VITEK 2 Compact for the Susceptibility of Carbapenem-Resistant Klebsiella Peumoniae to Amikacin
文章编号:
1671-7414(2018)01-133-04
作者:
刘 云万玉香马 炜李亚周黄晓春秦 琴
海军军医大学第一附属医院实验诊断科,上海 200433
Author(s):
LIU YunWAN Yu-xiangMA WeiLI Ya-zhouHUANG Xiao-chunQIN Qin
Department of Laboratory Diagnosis, the First Affiliated Hospital of Navy Military Medical University,Shanghai 200433,China
关键词:
VITEK 2 Compact 耐碳青霉烯类肺炎克雷伯菌 阿米卡星 纸片扩散法 E-test法
分类号:
R378.996; R446.5
DOI:
10.3969/j.issn.1671-7414.2018.01.001
文献标志码:
A
摘要:
目的 评价VITEK 2 Compact全自动微生物分析系统(简称VITEK 2 Compact)及与之配套的AST-GN13卡检测耐碳青霉烯类肺炎克雷伯菌对阿米卡星药物敏感性结果的准确性。方法 分别采用VITEK 2 Compact,纸片扩散法(K-B法)和E-test法检测临床分离的147株耐碳青霉烯类肺炎克雷伯菌对阿米卡星的敏感性。结果 147株耐碳青霉烯类肺炎克雷伯菌对阿米卡星的药敏检测中,K-B法和E-test法的结果完全一致。其中,5株VITEK 2 Compact,K-B法和E-test法三种检测方法结果一致,4株均为敏感,1株均为耐药; 142株VITEK 2 Compact和E-test法结果不一致,VITEK 2 Compact结果敏感或中介,而E-test法和K-B法结果耐药。结论 VITEK 2 Compact在检测耐碳青霉烯类肺炎克雷伯菌对阿米卡星的敏感性时会出现不准确的结果,临床工作中需要用K-B法或者其他方法验证后方能报告耐碳青霉烯类肺炎克雷伯菌对阿米卡星的药敏结果。
Abstract:
Abstract:Objective To evaluate the accuracy of VITEK 2 Compact in the detection of the drug susceptibility of carbapenem-resistant Klebsiella pneumoniae(CRKP)to Amikacin.Methods The susceptibility for 147 isolates of SRKP to Amikacin were detected by VITEK 2 Compact,disk diffusion method(K-B method)and E-test method,respectively.Results Among the 147 strains of Klebsiella pneumoniae,thedrug susceptibility results were consistent between K-B and E-test method.Among the 5 strains,the results were totally consistent by VITEK 2 Compact,K-B method and E-test method,of which 4 strains were sensitive,1 strains were resistant.There were 142 strains being not consistent between VITEK 2 Compact and E-test method.The results of VITEK 2 Compact were sensitive or intermediate,while E-test were drug resistance.Conclusion VITEK 2 Compact is not reliable for the detection of CRKP to Amikacin,which requires thatK-B method or other methods should be used for the susceptibility of CRKP to Amikacin.

参考文献/References:

[1] 倪语星,尚 红.临床微生物学检验[M].5版.北京:人民卫生出版社,2012:121-123. Ni YX,Shang H.Clinical microbiology test[M].5th Ed.Beijing:People's Publishing House,2012:121-123.
[2] 杨银梅,陈 斌,彭颖仪,等.VITEK 2 Compact全自动微生物分析系统检测亚胺培南耐药鲍曼不动杆菌对阿米卡星的准确性评估[J].检验医学,2016,31(10):907-910. Yang YM,Chen B,Peng YY,et al.Acuuracy of VITEK-2 compact for the determination of imipenemresistant Acinetobacter baumannii to Amikacin[J].Laboratory Medicine,2016,31(10):907-910.
[3] Clinical and Laboratory Standards Institute.Performance stardards for antimicrobial susceptibility testing[S].Wayne:PA,CLSI M100-S26:2016.
[4] Vardakas KZ,Matthaiou DK,Falagas ME,et al.Cha-racteristics,risk factors and outcomes of carbapenem-resistant Klebsiella pneumoniae infections inthe intensive care unit[J].J Infect,2015,70(6):592-599.
[5] 张河林,赵飞俊,何凤屏,等.2 527株临床分离病原菌的耐药性分析[J].现代检验医学杂志,2015,30(2):87-90. Zhang HL,Zhao FJ,He FP,et al.Drug resistance spectrum analysis of 2 527 clinical isolates of bacteria[J].J Mod Lab Med,2015,30(2):87-90.
[6] Khatri A,Naeger Murphy N,Wiest P,et al.Community-acquired pyelonephritis in pregnancy caused by KPC-producing Klebsiela pneumoniae[J].Antimicrob Agents Chemother,2015,59(8):4375-4378.
[7] 程 莉,谭婷婷,魏红霞,等.临床分离的耐碳青霉烯肺炎克雷伯菌的耐药机制研究[J].现代检验医学杂志,2017,32(3):112-114. Cheng L,Tan TT,Wei HX,et al.Resistance mechanisms of clinical isolated carbapenem-resistant Klebsiella pneumoniae[J].J Mod Lab Med,2017,32(3):112-114.
[8] 杨 朵,胡智颖,辛续丽.碳青霉烯类耐药肺炎克雷伯菌中KPC基因分布[J].中国感染与化疗杂志,2016,16(4):465-467. Yang D,Hu ZY,Xin XL.Prevalence of KPC gene in carbapenem-resistant Klebsiella pneumoniae isolates[J].Chin J Infect Chemother,2016,16(4):465-467.
[9] 黄晓春,陈 岩,李 园,等.VITEK 2C检测鲍曼不动杆菌对阿米卡星体外敏感性的性能评价[J].检验医学,2013,28(8):666-670. Huang XC,Chen Y,Li Y,et al.Evaluation on the performance of VITEK 2C for the susceptibility of Acinetobacter baumannii to Amikacin[J].Laboratory Medicine,2013,28(8):666-670.
[10] Bremmer DN,Clancy CJ,Press EG,et al.KPC-producing Klebsiella pneumoniae strains that harbor AAC(6')-Ib exhibit intermediate resistanceto amikacin[J].Antimicrob Agents Chemother,2014,58(12):7597-7600.
[11] Chiem K,Fuentes BA,Lin DL,et al.Inhibition of aminoglycoside 6'-N-acetyltransferase type Ib-mediated amikacin resistance in Klebsiella pneumoniae by zinc and copper pyrithione[J].Antimicrob Agents Chemother,2015,59(9):5851-5853.
[12] Haldorsen BC,Simonsen GS,Sundsfjord A,et al.Increased prevalence ofaminoglycoside resistance in clinical isolates of Escherichia coli and Klebsiella spp.In Norway is associated with the acquisition of AAC(3)-II and AAC(6')-Ib[J].Diagnostic Microbiology and Infectious Disease,2014,78(1):66-69. 收稿日期:2017-11-02 修回日期:2017-11-24

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备注/Memo

备注/Memo:
基金项目:上海青年临床医技人才(临床检验专业)培养资助计划(沪医卫基【2016】04号); 上海市科委项目(17JC1400900); 国家自然科学基金青年项目(31500721)。 作者简介:刘 云(1984-),女,硕士,主管技师,研究方向:临床微生物检验,E-mail:liuyun1258@163.com。 万玉香(1990-),共同第一作者,女,本科,技师,E-mail:13564570079@163.com。 通讯作者:秦 琴,Email: qinq78@163.com; 黄晓春,共同通讯作者,E-mail:chunxiao_84@hotmail.com。
更新日期/Last Update: 2018-02-07