[1]冯 娜,兰 静,彭 鹏,等.快速去除冰冻红细胞甘油的方法改进研究[J].现代检验医学杂志,2018,33(02):105-107.[doi:10.3969/j.issn.1671-7414.2018.02.001]
　FENG Na,LAN Jing,PENG Peng,et al.Establishing A Method to Rapidly Remove Glycerol in Frozen Red Blood Cells[J].Journal of Modern Laboratory Medicine,2018,33(02):105-107.[doi:10.3969/j.issn.1671-7414.2018.02.001]
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快速去除冰冻红细胞甘油的方法改进研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年02期
页码:
105-107
栏目:
研究简报·实验技术
出版日期:
2018-04-05

文章信息/Info

Title:
Establishing A Method to Rapidly Remove Glycerol in Frozen Red Blood Cells
文章编号:
1671-7414(2018)02-105-03
作者:
冯 娜; 兰 静; 彭 鹏; 王 锦; 刘 娜; 张 嫄; 赵京文
陕西省血液中心/西安市中心血站,西安 710061
Author(s):
FENG Na; LAN Jing; PENG Peng; WANG Jin; LIU Na; ZHANG Yuan; ZHAO Jing-wen
Shaanxi Blood Center/Xi'an Central Blood Station,Xi'an 710061,China
关键词:
去甘油;  冰冻红细胞;  三步法
分类号:
R457.12
DOI:
10.3969/j.issn.1671-7414.2018.02.001
文献标志码:
A
摘要:
目的 建立冰冻红细胞快速去甘油的方法。方法 采用三步法快速去除冰冻红细胞中的甘油。通过控制制备时盐水的浓度、作用时间及离心次数,用高浓度盐水大量稀释冰冻红细胞后,离心去除盐水,加入相应体积的生理盐水重悬,来达到快速去除甘油的目的。分别对比了常规法和改良法制备的冰冻解冻去甘油红细胞的血红蛋白含量、游离血红蛋白含量、甘油残留量、白细胞残留量及血细胞比容5个方面的指标。结果 传统去甘油的方法大约需要1 h,三步法去甘油大约需要15 min,极大地缩短了时间,提高了效率。对去甘油红细胞进行相关检测,甘油残留量和白细胞残留量符合《全血与成分血质量要求(2012版)》的规定,血红蛋白含量略低于规定。结论 快速解冰冻去甘油方法具有时间短、效率高的优点,但仍需进一步改进和优化以使产品质量全面达到国家标准。
Abstract:
Abstract:Objective To establish a method to rapidly remove glycerol in frozen red blood cells(RBC).Methods The glycerol in frozen RBC was removed by using three-step method as following:thefrozen RBC were diluted by adding salt solution with high concentration.The salt solution was removed through centrifugation.The RBC were resuspended in normal saline of corresponding volume.Three-step method could achieve the quick removal of glycerol by controlling the concentration salt water and action time.The indexes of hemoglobin,free hemoglobin,glycerol residue,residual white bloodcell(WBC)and hematocrit of frozen-deglycerolized RBC were compared between conventional and improved methods.Results The traditionalmethod to remove glycerol needed 1h approximately,while the three-step methodonly needed 15 mins approximately,greatly shortening the time and improving theefficiency.The related tests for removing glycerol RBC,except hemoglobin content was slightly lower than the “The quality requirements for whole blood and blood components(2012 edition)”,the rest of them all meeted the requirements.Conclusion The three-step method has the advantages of shorttime and high efficiency.However,it still needs to be further improved and optimized to make the quality of product meet the national standard.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:冯 娜(1982-),女,公共卫生硕士,主管技师,主要从事血液质量管理。 通讯作者:赵京文(1967-),男,大学本科,主任医师,从事血液管理。
更新日期/Last Update: 2018-04-16