[1]武爱荣,安 良,黄 波,等.VITEK-2 Compact AST-GN16检测鲍曼不动杆菌对阿米卡星和哌拉西林-他唑巴坦药敏结果的准确性评价[J].现代检验医学杂志,2018,33(06):136-139.[doi:10.3969/j.issn.1671-7414.2018.06.035]
　WU Ai-rong,AN Liang,HUANG Bo,et al.Evaluation of the Accuracy of Acinetobacter Baumannii's Susceptibility to Amikacin and Peracillin-Tarazobatam in the Detection of Vitek-2 Compact AST-GN16[J].Journal of Modern Laboratory Medicine,2018,33(06):136-139.[doi:10.3969/j.issn.1671-7414.2018.06.035]
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VITEK-2 Compact AST-GN16检测鲍曼不动杆菌对阿米卡星和哌拉西林-他唑巴坦药敏结果的准确性评价()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第33卷
期数:
2018年06期
页码:
136-139
栏目:
研究简报·实验技术
出版日期:
2018-12-24

文章信息/Info

Title:
Evaluation of the Accuracy of Acinetobacter Baumannii's Susceptibility to Amikacin and Peracillin-Tarazobatam in the Detection of Vitek-2 Compact AST-GN16
文章编号:
1671-7414(2018)06-136-04
作者:
武爱荣; 安 良; 黄 波; 马霄婷
西安高新医院检验科,西安 710075
Author(s):
WU Ai-rong; AN Liang; HUANG Bo; MA Xiao-ting
Department of Clinical Laboratories,Xi'an Gaoxin Hospital,Xi'an 710075,China
关键词:
VITEK-2 Compact AST-GN16;  鲍曼不动杆菌;  阿米卡星;  哌拉西林-他唑巴坦
分类号:
R378; R446.5
DOI:
10.3969/j.issn.1671-7414.2018.06.035
文献标志码:
A
摘要:
目的 探讨VITEK-2 Compact AST-GN16检测鲍曼不动杆菌对阿米卡星(AK)和哌拉西林-他唑巴坦(TZP)药敏结果的准确性。方法 收集2017年5月～2018年5月临床分离的100株鲍曼不动杆菌,分别采用E-test法,纸片扩散法(K-B法)和VITEK 2 Compact AST-GN16药敏卡检测分离菌株对AK和TZP的敏感性。结果 100株鲍曼不动杆菌中,有20株对IPM敏感,80株对IPM耐药。对IPM敏感的鲍曼不动杆菌,3种方法检测的AK和TZP敏感性结果一致,均为敏感,差异无统计学意义(P<0.05)。对80株耐IPM的鲍曼不动杆菌,AK药敏结果显示:E-test法结果共有48株耐药,3株中介,29株敏感; K-B法结果有78株与E-test法结果一致,另外2株耐药; 有35株3种方法结果一致,45株VITEK法为敏感或中介,而其它两种方法均为耐药。E-test法和K-B法标准符合率(CA)为97.5%,VITEK法与E-test法CA为11.25%,一般错误率为2.5%,严重错误率为1.25%,极严重错误率为17.5%。TZP药敏结果显示:E-test法和K-B法结果完全一致,均为耐药; VITEK法有75株耐药,5株中介。E-test法和K-B法CA为100%,VITEK法与E-test法CA为93.75%,一般错误率为6.25%。结论 VITEK-2 Compact AST-GN16检测IPM敏感鲍曼不动杆菌对AK和TZP敏感性结果可靠,无需验证; 但检测耐IPM鲍曼不动杆菌对AK和TZP的敏感性时,会出现不同程度的错误结果,在工作中需采用E-test法或K-B法进行验证。
Abstract:
Objective To investigate the accuracy of VITEK-2 Compact AST-GN16 detection of Acinetobacter baumannii(Ab)for the sensitivity of amikacin(AK)and piperacillin(TZP).Methods 100 strains of Ab clinically isolated from May 2017 to May 2018 were collected.Sensitivity of the isolated strain to AK and TZP was detected by E-test, paperdiffusion(K-B)and VITEK 2 Compact AST-GN16.Results Among 100 strains of Ab,20 were sensitive to IPM and 80 were resistant to IPM.For IPM-sensitive Ab,the sensitivity results of AK and TZP detected by the three methods were consistent,both of which were sensitive and the differences werenot statistically significant(P<0.05).For 80 IPM-resistant Ab,resultsof AK drug sensitivity showed that there were 48 strains of drug resistance,3mediators and 29 strains of sensitivity.The results of K-B method were consistent with those of E-test,and the other two were resistant.The results of 3 methods of 35 strains were consistent,45 strains of VITEK were sensitive or intermediate,while the other two methods were resistant.The standard compliance rate(CA)of E-test and K-B method was 97.5%,while that of VITEK and E-test,CA was 11.25%,with a general error rate of 2.5%,a serious error rate of 1.25% and a very serious error rate of 17.5%.TZP drug sensitivity results showed that the results of E-test and K-B were identical,both of which were drug resistance.There were 75 resistant strains and 5 mediators by VITEK.The standardcompliance rate(CA)of E-test and K-B was 100%,VITEK and E-test CA was 93.75%,and the general error rate was 6.25%.Conclusion Detection of IPN-sensitive Ab with sensitivity to AK and TZP was reliable withoutverification.However,when the sensitivity of IPN-resistant Ab to AK and TZPwas detected,error results of different degrees will appear.E-test method orK-B method should be used for verification in the work.

参考文献/References:

[1] 刘 云,万玉香,马 炜,等.VITEK 2 Compact检测耐碳青霉烯类肺炎克雷伯菌对阿米卡星药敏结果的准确性评价[J].现代检验医学杂志,2018,33(1):133-136.
Liu Y,Wan YX,Ma W,et al.Evaluation on the accuracy of VITEK 2 Compact for the susceptibility of carbapenem-resistant Klebsiella pneumoniaeto amikacin[J].Journal of Modern Laboratory Medicine,2018,33(1):133-136.
[2] 杨银梅,陈 斌,彭颖仪,等.VITEK 2 Compact全自动微生物分析系统检测亚胺培南耐药鲍曼不动杆菌对阿米卡星的准确性评估[J].检验医学,2016,31(10):907-910.
Yang YM,Chen B,Peng YY,et al.Accuracy of VI-TEK 2 Compact for the determination of imipenem resistant Acinetobacter baumannii to amikacin[J].Laboratory Medicine,2016,31(10):907-910.
[3] Clinical and Laboratory Standards Institute.Performance standards for antimicrobial susceptibility testing[S].Twenty-Sixth Edition.Wayne:PA,CLSI-M100S,2016.
[4] Clinical and Laboratory Standards Institute.Development of in vitro susceptibility testing criteria and quality control parameters for veterinary antimicrobial agents:approved guideline[S].Wayne:PA,CLSI-M23-A2,2008.
[5] Peleg AY,Seifert H,Paterson DL.Acinetobacter baumannii:emergenceof a successful pathogen[J].Clin Microbiol Rev,2008,21(3):538-582.
[6] 郭 洁,徐 杰,王丽丽,等.耐碳青霉烯类抗生素鲍曼不动杆菌的耐药谱分析[J].现代检验医学杂志,2012,27(1):107-109.
Guo J,Xu J,Wang LL,et al.Antibiotics resistance profiles of carbapene-resistant acinetobacter baumannii isolates[J].Journal of Modern Laboratory Medicine,2012,27(1):107-109.
[7] 苍金荣,王 华,张利侠,等.多黏菌素B、头孢哌酮/舒巴坦对泛耐药鲍曼不动杆菌体外抗菌活性观察[J].现代检验医学杂志,2011,26(5):147-148.
Cang JR,Wang H,Zhang LX,et al.Observation of in vitro antibacterial activity of pandrug-resistant Acinetobacter baumannii by polymyxin B and cefoperazone/sulbactam[J].Journal of Modern Laboratory Medicine,2011,26(5):147-148.
[8] 王 启,季 萍,徐修礼,等.2016年中国14家教学医院革兰阴性杆菌耐药监测分析[J].中华检验医学杂志,2017,40(8):614-622.
Wang Q,Ji P,Xu XL,et al.Antimicrobial resistance of gram-negative bacilli isolated from 14 teaching hospitals across China in 2016[J].Chinese Journal of Laboratory Medicine,2017,40(8):614-622.
[9] 黄 烈,张银辉,聂署萍,等.全自动微生物分析仪检测细菌耐药表型性能评价[J].国际检验医学杂志,2012,33(5):602-604.
Huang L,Zhang YH,Nie SP,et al.Performance evaluation of bacterial resistance phenotype detected by automatic microbiological analyzer[J].International Journal of Laboratory Medicine,2012,33(5):602-604.
[10] 王 莹,汪鹏程,曾章锐,等.Vitek 2 Compact全自动微生物仪检测鲍曼不动杆菌对阿米卡星敏感性误差的原因[J].临床检验杂志,2013,31(1):28-31.
Wang Y,Wang PC,Zeng ZR,et al.Investigation on the source of errors forthe susceptibility of Acinetobacter baumannii to amikacin detected by Vitek2 Compact[J].Chinese Journal of Clinical Laboratory Science,2013,31(1):28-31.
[11] 张静会.鲍曼不动杆菌流行现状及耐药性分析[J].实验与检验医学,2017,35(6):918-920.
Zhang JH.Current status and drug resistance analysis of acinetobacter baumannii[J].Experimental and Laboratory Medicine,2017,35(6):918-920.
[12] 赵建平,张叶毛,武 芳,等.神经科病房鲍曼不动杆菌的分布及耐药性分析[J].中国感染与化疗杂志,2015,15(1):60-62.
Zhao JP,Zhang YM,Wu F,et al.Analysis of distribution and drug resistance of Acinetobacter baumannii in neurology ward[J].Chinese Journal of Infection and Chemotherapy,2015,15(1):60-62.
[13] 闻海丰,赵建宏,时东彦,等.河北省细菌耐药监测网耐药数据质量分析:鲍曼不动杆菌对阿米卡星耐药数据问题初步探讨[J].中国抗生素杂志,2016,41(7):557-560,S1.
Wen HF,Zhao JH,Shi DY,et al.Quality analysis of data collected by Hebei provincial antimicrobial resistant investigation net:Preliminary discussion of the resistance rate of Acinetobacter baumannii to amikacin[J].Chinese Journal of Antibiotics,2016,41(7):557-560,S1.
[14] 张晓文,邵海枫,王卫萍,等.氨基糖苷类药物双圈耐药型鲍曼不动杆菌16S rRNA甲基化酶基因研究[J].中国抗生素杂志,2011,36(11):855-858.
Zhang XW,Shao HF,Wang WP,et al.A study of 16S rRNA methylase on Acinetobacter baumannii double-circle resistance to aminoglycoside antibiotic[J].Chinese Journal of Antibiotics,2011,36(11):855-858.
[15] 温海楠,谢守军,赵建宏,等.鲍曼不动杆菌对8种常用抗菌药物敏感试验不同方法的比较[J].中国感染与化疗杂志,2017,17(6):680-684.
Wen HN, Xie SJ, Zhao JH,et al.Susteptibility of Acinetobacter baumannii isolates to eight antimicrobial agents tested by different methods[J].Chinese Journal of Infection and Chemotherapy,2017,17(6):680-684.

备注/Memo

备注/Memo:
作者简介:武爱荣(1976-),女,学士学位,主管检验师,主要从事病原微生物感染诊断,E-mail:wu airong@126.com。
更新日期/Last Update: 2018-11-30