[1]屈晨虹a,丁 雨b,史伟峰b.MADLI-TOF MS 和PhoenixTM-100 两种分析仪鉴定肠杆菌科细菌一致性评价[J].现代检验医学杂志,2020,35(02):97-99.[doi:10.3969 / j.issn.1671-7414.2020.02.027]
 QU Chen-honga,DING Yub,SHI Wei-fengb.Consistency of MADLI-TOF MS and PhoenixTM-100 to IdentifyEnterobacteriaceae Bacteria[J].Journal of Modern Laboratory Medicine,2020,35(02):97-99.[doi:10.3969 / j.issn.1671-7414.2020.02.027]
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MADLI-TOF MS 和PhoenixTM-100 两种分析仪鉴定肠杆菌科细菌一致性评价()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年02期
页码:
97-99
栏目:
研究简报·实验技术
出版日期:
2020-05-24

文章信息/Info

Title:
Consistency of MADLI-TOF MS and PhoenixTM-100 to IdentifyEnterobacteriaceae Bacteria
文章编号:
1671-7414(2020)02-097-04
作者:
屈晨虹a 丁 雨b史伟峰b
(苏州大学附属第三医院a. 输血科;b. 检验科,江苏常州213003)
Author(s):
QU Chen-honga DING Yub SHI Wei-fengb
(a. Department of Blood Transfusion;b.Department of Clinical Laboratory,the Third Affiliated Hospital of Soochow University,Jiangsu Changzhou 213003,China)
关键词:
耐碳青霉烯类肠杆菌基质辅助激光解析电离飞行时间质谱细菌鉴定
分类号:
R446
DOI:
10.3969 / j.issn.1671-7414.2020.02.027
文献标志码:
A
摘要:
目的 评价德国Bruker 公司的基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS) 和美国BD 公司的菲尼克斯100(PhoenixTM-100) 细菌鉴定仪对临床分离肠杆菌科细菌鉴定的一致性。方法 回顾性分析2017 年1 月~ 2019年8 月苏州大学附属第三医院从临床各类标本中分离出的肠杆菌科细菌1 242 株,MALDI-TOF MS 和PhoenixTM-100 细菌鉴定仪对这些菌株进行鉴定,运用统计学方法分析并评估两种仪器的鉴定结果的一致性。结果 在两台仪器均能鉴定到属水平的1 242 株细菌中,鉴定到菌种水平的一致率达85.1% (1 057 株)。在8 株肺克鼻臭亚种菌种的鉴定上,PhoenixTM-100 鉴定出6 株,MALDI-TOF MS 鉴定出2 株,两组间的鉴定比较差异有统计学意义(χ2=4.00,P=0.046),在其他种水平上的鉴定结果差异无统计学意义(χ2=0.207~4.000, 均P > 0.05)。沙门菌属均未能鉴定到种水平。结论 两种仪器都能准确鉴定肠杆菌科细菌到属水平,可用于临床微生物实验室对临床标本的常规鉴定,但对肺克鼻臭亚种和沙门菌属并不能精准的鉴定到种水平。

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备注/Memo

备注/Memo:
作者简介:屈晨虹(1990-),女,本科,检验师,从事临床微生物学和免疫学研究,E-mail:quchenhongnew@163.com。通讯作者:史伟峰,男,副教授,硕士生导师,主要从事临床微生物学研究,E-mail:swf67113@163.com。收稿日期:2019-11-11 修回日期:2019-11-30
更新日期/Last Update: 2020-04-30