[1]柴春艳,王 甜,刘娅萍,等.支气管肺癌患者血清和癌组织中瘦素水平的表达及临床意义[J].现代检验医学杂志,2020,35(04):18-21,49.[doi:10.3969/j.issn.1671-7414.2020.04.005]
　CHAI Chun-yan,WANG Tian,Liu Ya-ping,et al.Expression and Clinical Significance of Leptin in Serum and Cancer Tissues of Patients with Bronchial Lung Cancer[J].Journal of Modern Laboratory Medicine,2020,35(04):18-21,49.[doi:10.3969/j.issn.1671-7414.2020.04.005]
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支气管肺癌患者血清和癌组织中瘦素水平的表达及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年04期
页码:
18-21,49
栏目:
论　著
出版日期:
2020-09-05

文章信息/Info

Title:
Expression and Clinical Significance of Leptin in Serum and Cancer Tissues of Patients with Bronchial Lung Cancer
文章编号:
1671-7414（2020）04-018-05
作者:
柴春艳1;  王 甜1;  刘娅萍1;  徐邦强1;  赵 媛1;  李雅莉2
（1. 陕西省人民医院西院一病区，西安710068；2. 西安交通大学第二附属医院呼吸内科，西安 710004）
Author(s):
CHAI Chun-yan1; WANG Tian1; Liu Ya-ping1; XU Bang-qiang1; ZHAO Yuan1; LI Ya-li2
(1.Shaanxi Provincial People’s Hispital, Xi’an 710068,China; 2.Department of Respiratory Medicine,the Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004,China)
关键词:
肺肿瘤;  免疫组织化学;  瘦素;  蛋白表达
分类号:
R734.2；R730.43
DOI:
10.3969/j.issn.1671-7414.2020.04.005
文献标志码:
A
摘要:
目的 研究瘦素（Leptin）在支气管肺癌患者血清和肺癌组织中的表达及临床意义。方法 118 例肺癌患者( 肺癌组) 和24 例正常人( 对照组) 的血清Leptin 水平采用酶联免疫法（ELISA）测定，118 例手术切除的肺癌标本及24例正常肺组织中的Leptin 表达情况采用免疫组织化学SP 法检测。结果 肺癌组患者血清瘦素(3.6±1.5μg/L) 水平显著高于对照组(1.6±0.7μg/L)，差异有统计学意义(t=3.485，P=0.002)。肺癌患者肺组织中瘦素表达的阳性率为75.42%（89/118），正常肺组织瘦素表达的阳性率为25.0%（6/24），两者比较差异有统计学意义（P<0.05）。男性肺癌患者瘦素的表达阳性率为95.0%（57/60），女性为55.17%（32/58），两者相比差异有统计学意义(t=25.237，P=0.000)。支气管肺癌患者的年龄、病理类型对Leptin 表达阳性率的影响无显著性差异(χ2=0.095，0.013，均P ＞ 0.05)。肺癌患者的组织分化程度、临床分期、有无淋巴结转移对Leptin 表达阳性率的影响有显著性统计学差异(χ2=7.249，P<0.05)。吸烟指数≥ 400 的肺癌患者瘦素表达阳性率68.54%（61/89），吸烟指数＜ 400 的患者为31.46%（28/89），两者相比差异有统计学意义(χ2=12.44，P=0.000)。结论 肺癌患者的血清Leptin 水平显著高于正常人的血清Leptin 水平。支气管肺癌组织中Leptin 蛋白高表达，Leptin 的表达与肺癌患者的年龄、病理类型无关，与肺癌患者性别、组织的分化程度、临床分期、有无淋巴结转移、吸烟指数密切相关。

参考文献/References:

[1] 胡森安，艾红红，严丽花，等．维生素D 及日照量对单纯性肥胖儿童血清瘦素水平影响的研究[J]．现代检验医学杂志，2016, 31(6): 143-146． HU Senan, AI Honghong, YAN Lihua, et al. Effect ofvitamin D and sunlight on serum leptin levels in simpleobese children[J]. Journal of Modern LaboratoryMedicine , 2016, 31(6): 143-146.
[2] RODRIGUES P R, MAIA L L, SANTOS M, etal. Leptin receptor expression and Gln223Argpolymorphism as prognostic markers in oral andoropharyngeal cancer[J]. Genetics and MolecularResearch, 2015, 14(4): 14979-14988.
[3] NTIKOUDI E, KIAGIA M, BOURA P, et al. Hormonesof adipose tissue and their biologic role in lungcancer[J]. Cancer Treatment Reviews, 2014, 40(1): 22-30.
[4] FENG Helin,LIU Qingyi,ZHANG Ning, et al. Leptinpromotes metastasis by inducing an epithelialmesenchymaltransition in A549 lung cancer cells[J].Oncology Research, 2013, 21(3): 165-171.
[5] RAGHU A, TAN B K, RANDEVA H S. Differentialeffects of leptin and adiponectin in endothelialangiogenesis[J]. Journal of Diabetes Research, 2015,2015: 648239.
[6] VAN DEN BORST B, SOUREN N Y P, LOOS R J F,et al. Genetics of maximally attained lung function: arole for leptin[J]. Respir Med, 2012, 106(2): 235-242.
[7] LEE S M, CHOI H J, OH C H, et al. Leptinincreases TNF-α expression and production throughphospholipase D1 in raw 264.7 cells[J]. PLoS One,2014, 9(7): e102373.
[8] 李明安，司丽媛，吴红波，等．哮喘患儿外周血单个核细胞瘦素及Foxp3 的表达[J]．现代检验医学杂志, 2016, 31（4）: 58-61, 64． LI Mingan, SI Liyuan, WU Hongbo, et al. Expressionof leptin and Foxp3 in peripheral blood mononuclearcells in children with bronchial asthma[J]. Journal ofModern Laboratory Medicine , 2016,31(4): 58-61, 64.
[9] 胡森安，艾红红，梁惠霞，等．早发冠心病患者红细胞分布宽度与血清瘦素水平及其相关性分析[J]．现代检验医学杂志, 2017, 32(1)：91-94．HU Senan, AI Honghong, LIANG Huixia, et al.Analysis of the relationship between red blood celldistribution width and serum leptin level in patientswith premature coronary artery disease[J]. Journal ofModern Laboratory Medicine, 2017, 32(1):91-94.
[10] PATHAK R R, GROVER A, MALANEY P, et al. Lossof tumor suppressor PTEN induces leptin mediatedleptin gene expression,A feed-forward loop operatingin the lung[J]. Journal of Biological Chemistry, 2013,288(41): 29821-29835.
[11] WANG Wei,YAN Haicheng,DOU Changwu,et al.Human leptin triggers proliferation of A549 cellsvia blocking endoplasmic reticulum stress-relatedapoptosis[J]. Biochemistry,2013,78(12):1333-1341.
[12] LI Yuliang ,GENG Jianli ,WANG Yongzheng ,et al.The role of leptin receptor gene polymorphisms indetermining the susceptibility and prognosis of NSCLCin Chinese patients[J]. Journal of cancer research andclinical oncology,2012,138(2): 311-316.
[13] WERYNSKA B, KOSACKA M, GO.ECKI M, etal. Serum levels in cachectic and non-cachectic lungcancer patients[J]. Pneumonologia I Alergologia Polska,2009, 77(6): 500-506.
[14] 陶晓南，张晓菊，金阳，等．肺癌患者血清瘦素和肿瘤坏死因子-α 的检测及其相关性分析[J]．临床内科杂志, 2013, 20(8)：416-418． TAO Xiaonan, ZHANG Xiaoju, JIN Yang, et al.Detection of serum leptin ,TNF-α and their correlatedanalysis in patients with lung cancer[J]. Journal ofClinical Internal Medicine, 2013, 20(8):416-418.
[15] 冯和林，郭鹏，王进，等．瘦素及其受体在肺腺癌组织中的表达及其与骨转移的关系[J]．中华肿瘤杂志，2016，38（11）：840-844． FENG Helin, GUO Peng, WANG Jin, et al. Associationof the expression of leptin and leptin receptor with bonemetastasis in pulmonary adenocarcinoma [J]. ChineseJournal of Oncology, 2016, 38(11):840-844.

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备注/Memo

备注/Memo:
收稿日期：2020-03-16修回日期：2020-04-28基金项目：陕西省重点研发计划项目（项目编号: 2020SF100）；陕西省自然科学基础研究计划项目（项目编号：2018JM3035）。作者简介：柴春艳(1982-)，女，硕士，主治医师，研究方向：肺癌的早期诊断及治疗，E-mail：swallowchai3412@163.com。通讯作者：赵媛（1973-），女，博士，主任医师，研究方向：老年内分泌代谢性疾病的诊治，E-mail：383638940@qq.com。
更新日期/Last Update: 2020-10-08