[1]崔俊华,王蓓丽,周佳烨,等.三种试剂检测血清幽门螺杆菌抗体的比较研究[J].现代检验医学杂志,2021,36(01):105-107.[doi:10.3969/j.issn.1671-7414.2021.01.026]
　CUI Jun-hua,WANG Bei-li,ZHOU Jia-ye,et al.Comparative Study on Three Reagents for Detecting Helicobacter Pylori Infection[J].Journal of Modern Laboratory Medicine,2021,36(01):105-107.[doi:10.3969/j.issn.1671-7414.2021.01.026]
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三种试剂检测血清幽门螺杆菌抗体的比较研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年01期
页码:
105-107
栏目:
研究简报 实验技术
出版日期:
2021-02-05

文章信息/Info

Title:
Comparative Study on Three Reagents for Detecting Helicobacter Pylori Infection
文章编号:
1671-7414（2021）01-105-04
作者:
崔俊华; 王蓓丽; 周佳烨; 等
（1. 复旦大学附属中山医院检验科，上海 200032；2. 复旦大学附属中山医院厦门医院检验科，福建厦门 361015）
Author(s):
CUI Jun-hua;  WANG Bei-li;  ZHOU Jia-ye;  et al
(1. Department of Laboratory Medicine, Zhongshan Hospital, Fudan University , Shanghai 200032, China; 2. Department of Laboratory Medicine, Xiamen Branch, Zhongshan Hospital, Fudan University, Fujian Xiamen 361015,China)
关键词:
幽门螺杆菌抗体; 血清学; 检测方法
分类号:
R378.2；R446
DOI:
10.3969/j.issn.1671-7414.2021.01.026
文献标志码:
A
摘要:
目的　对三种用于检测血清中的幽门螺杆菌（ Helicobacter pylori，Hp）抗体的试剂进行比较研究。方法　于 2017年 4月采集未经抗 Hp治疗并接受快速尿素酶试验（ rapid urease test，RUT）和尿素 13C呼气试验（ 13C urease breath test， 13C-UBT）的 80例患者血清样本，用三种不同试剂—— A试剂：胶体金法幽门螺杆菌尿素酶抗体检测试剂盒， B试剂：胶乳增强免疫比浊法幽门螺杆菌检测试剂盒， C试剂抗幽门螺杆菌 IgG ELISA试剂盒检测血清 Hp抗体。以 RUT和 13C-UBT任何一项阳性为 Hp感染诊断标准，评估 A, B和 C三种试剂的检测性能，包括敏感度、特异度、准确度以及与诊断的一致性。结果　敏感度由高到低依次是 C（92.50%），B（78.57%）和 A（57.14%）；特异度由高到低依次是 A（100.00%），C（75.00%）和 B（70.83%）；准确度由高到低依次是 C（88.75%），B（76.25%）和 A（70.00%）；三种试剂检测结果之间存在差异。经 Kappa检验，C试剂与 RUT和 13C-UBT检测结果一致性最高（κ =0.722, P<0.001）。结论　三种血清 Hp抗体试剂检测结果与 RUT和 13C-UBT检测结果存在相关性，其中 C试剂一致性最高，可用于检测未经抗 Hp治疗患者的 Hp感染。

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（ 81772263，81972000，81902139）；2019厦门市医疗卫生重点项目（ YDZX20193502000002）；复旦大学附属中山医院院内临床研究项目（2018ZSLC05）；上海市临床重点专科建设项目（医学检验科）。
作者简介：崔俊华（1988-），男，学士，技师，研究方向：免疫， E-mail：cui.junhua@zs-hospital.sh.cn。
通讯作者：郭玮，主任技师，E-mail：guo.wei@zs-hospital.s
更新日期/Last Update: 2021-02-05