[1]郑　伟,宋晓雪,严　翔,等.人工远端肢体缺血再灌注对APAP诱导小鼠肝损伤的保护作用研究[J].现代检验医学杂志,2021,36(04):60-63.[doi:10.3969/j.issn.1671-7414.2021.04.013]
　ZHENG Wei,SONG Xiao-xue,YAN Xiang,et al.Effect of Artificial Remote Ischemia-Reperfusion Conditioning onAcetaminophen-Induced Acute Liver Injury in Mice and Its Possible Mechanism[J].Journal of Modern Laboratory Medicine,2021,36(04):60-63.[doi:10.3969/j.issn.1671-7414.2021.04.013]
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人工远端肢体缺血再灌注对APAP诱导小鼠肝损伤的保护作用研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年04期
页码:
60-63
栏目:
论　著
出版日期:
2021-07-31

文章信息/Info

Title:
Effect of Artificial Remote Ischemia-Reperfusion Conditioning onAcetaminophen-Induced Acute Liver Injury in Mice and Its Possible Mechanism
文章编号:
1671-7414（2021）04-060-05
作者:
郑　伟; 宋晓雪; 严　翔; 张智勇; 常虎林
（陕西省人民医院肝胆外科，西安 710068）
Author(s):
ZHENG Wei;  SONG Xiao-xue;  YAN Xiang;  ZHANG Zhi-yong;  CHANG Hu-lin
（Department of Hepatobiliary Surgery, Shaanxi Provincial People’s Hospital, Xi’an 710068, China）
关键词:
远端肢体缺血再灌注预处理; 远端肢体缺血再灌注后处理; 对乙酰氨基酚; 肝损伤; 小鼠
分类号:
R-332
DOI:
10.3969/j.issn.1671-7414.2021.04.013
文献标志码:
A
摘要:
目的　研究人工远端肢体缺血再灌注预处理（R-IPC）和缺血再灌注后处理（R-IPOST）对对乙酰氨基酚（APAP）诱导小鼠肝损伤的保护作用。方法　按随机数字表法将实验小鼠分为5 组：正常对照组（不做任何处理）、假手术组（缺血再灌注处理前后腹腔注射1ml 生理盐水）、APAP 组（腹腔注射1ml APAP 溶液）、R-IPC+APAP 组（缺血再灌注预处理后腹腔注射1ml APAP 溶液）、R-IPOST+APAP 组（腹腔注射1ml APAP 溶液后实施缺血再灌注后处理）。观察各组肝脏病理形态变化；检测各组血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST) 活性、肿瘤坏死因子-a（TNF-a）水平、白细胞介素-6（IL-6）水平；检测各组肝组织丙二醛（MDA）含量、超氧化物歧化酶（SOD）和谷胱甘肽酶（GSH）的水平和比较各指标组间差异。结果　光镜下R-IPC+APAP 组和R-IPOST+APAP 组肝小叶结构破坏程度及炎性细胞浸润程度较APAP 组均明显减轻。R-IPC+APAP 组血清ALT, AST, TNF-a, IL-6 和肝匀浆MDA 含量均明显低于APAP 组[（3 742±519.7 U/L, 3 471± 631.4U/L, 264.8±70.4pg/ml, 738.7 ± 71.0 pg/ml, 8.9± 1.2nmol/mg.prot）vs (5 564± 621.7U/L, 4 647± 813.9U/L, 351.7 ± 52.3pg/ml, 929.7±140.6pg/ml, 13.1± 1.7nmol/mg.prot)], 差异均有统计学意义（t =3.400~7.032, 均 P<0.05)；R-IPC+APAP 组肝匀浆SOD 活性明显高于APAP 组（11.0±1.9U/mg.prot vs 8.6± 1.1U/mg.prot)，差异有统计学意义（t ＝ 3.043, P<0.05）；R-IPOST+APAP 组血清ALT, AST, TNF-a, IL-6 和肝匀浆MDA 含量均明显低于APAP 组[(3 410± 588.6 U/L, 3 546±499.5U/L, 256.6±48.1pg/ml, 775.4±98.4pg/ml, 9.3±1.9nmol/mg.prot) vs(5 564±621.7U/L, 4 647±813.9U/L, 351.7±52.3pg/ml, 929.7±140.6pg/ml, 13.1± 1.7nmol/mg.prot)]，差异均有统计学意义（t ＝ 2.196~4.981, 均P<0.05）；R-IPOST +APAP 组肝匀浆GSH 活性明显高于APAP 组（10.3± 1.2U/mg.prot vs 7.9±0.6U/mg.prot）, 差异有统计学意义（t ＝ 3.702, P<0.05）。结论　R-IPC 和R-IPOST 能降低APAP 诱导的药物性肝损伤氧化应激和炎症反应程度，对肝功能具有保护作用。

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备注/Memo

备注/Memo:
基金项目：西安市科技计划医学项目（20YXYJ0010）。
作者简介：郑伟（1980-）男，硕士，主治医师，研究方向：药物性肝损伤的诊治，E-mail：zhengwei_xa@163.com。
通讯作者：常虎林，男，主治医师，研究方向：慢性肝病的诊治，E-mail:changhulin_spph@126.com。
更新日期/Last Update: 1900-01-01