[1]王新庄,孟建涛.慢性心力衰竭患者血清 lncRNA MALAT1的表达水平及其临床意义[J].现代检验医学杂志,2022,37(01):141-144.[doi:10.3969/j.issn.1671-7414.2022.01.028]
　WANG Xin-zhuang,MENG Jian-tao.Expression of lncRNA MALAT1 in Peripheral Blood of Patients with Chronic Heart Failure and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2022,37(01):141-144.[doi:10.3969/j.issn.1671-7414.2022.01.028]
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慢性心力衰竭患者血清 lncRNA MALAT1的表达水平及其临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年01期
页码:
141-144
栏目:
论　著
出版日期:
2022-01-15

文章信息/Info

Title:
Expression of lncRNA MALAT1 in Peripheral Blood of Patients with Chronic Heart Failure and Its Clinical Significance
文章编号:
1671-7414（2022）01-141-04
作者:
王新庄; 孟建涛
( 西安高新医院急诊科，西安 710077)
Author(s):
WANG Xin-zhuang;  MENG Jian-tao
(Department of Emergency, Xi’an Gaoxin Hospital, Xi’an 710077, China)
关键词:
慢性心力衰竭; 长链非编码 RNA肺癌转移相关转录本 1; 生物学标志物
分类号:
R541.6；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.01.028
文献标志码:
A
摘要:
目的　探究慢性心力衰竭 (chronic heart failure, CHF)患者血清长链非编码 RNA(long non-coding RNA, lncRNA)肺癌转移相关转录本 1(MALAT1)的表达水平及其临床意义。方法　选取 2017年 2月～ 2019年 5月西安高新医院急诊科收治的 62例 CHF患者为 CHF组，另选取同期 70例体检健康者为对照组。采用实时荧光定量 PCR(qRT-PCR)检测血清 lncRNA MALAT1的表达水平。采用酶联免疫吸附法 (ELISA)测定血清凋亡分子的水平。采用彩色多普勒超声仪检查并测定心力衰竭相关指标。相关性采用 Pearson相关性分析，采用受试者工作特征 (receiver operating characteristic, ROC)曲线评价 lncRNA MALAT1对 CHF的诊断效能。结果　CHF组患者血清 lncRNA MALAT1和 B淋巴细胞瘤 -2(B-cell lymphoma-2, Bcl-2)的水平均低于对照组， CHF组患者血清 Bcl-2相关 x蛋白 (BCL2-Associated X, Bax)水平高于对照组，差异均具有统计学意义 (t=16.31, 11.90, 9.57,均 P=0.00)。CHF组中美国纽约心脏病学会 (New York Heart Disease Assocation, NYHA)分级Ⅲ级和Ⅳ级患者血清 lncRNA MALAT1和 Bcl-2的水平低于 NYHAⅡ级， CHF组中 NYHA Ⅲ级和Ⅳ级患者血清 Bax水平高于 NYHAⅡ级， NYHAⅣ级患者血清 lncRNA MALAT1和 Bcl-2的水平低于Ⅲ级，血清 Bax水平高于Ⅲ级，差异均具有统计学意义 (均 P＜ 0.05)。CHF组患者左室射血分数 (left ventricular ejection fraction, LVEF)低于对照组，左室舒张末期内径 (left ventricular end diastolic diameter, LVEDD)和左室收缩末期内径 (left ventricular end systolic diameter, LVESD)高于对照组，差异均具有统计学意义 (t=4.56~10.85,均 P=0.00)。Pearson相关性分析显示， CHF组患者血清 lncRNA MALAT1和 Bcl-2水平与 LVEF呈正相关 (r=0.29，0.32，均 P＜ 0.05)，与 LVEDD和 LVESD呈负相关 (r=-0.25，-0.31，P=0.01)。CHF组患者血清 Bax与 LVEF呈负相关 (r=-0.33，P=0.01)，与 LVEDD和 LVESD呈正相关 (r=0.20，0.32，P=0.00)。CHF组患者血清 lncRNA MALAT1与 Bcl-2水平呈正相关 (r=0.31， P=0.00)，与血清 Bax水平呈负相关 (r=-0.24，P=0.01)。血清 lncRNA MALAT1诊断 CHF的曲线下面积 (area under the curve, AUC)为 0.791，敏感度和特异度分别为 75.0%和 79.5% (95%CI=0.653~0.930，P=0.001)。结论　CHF患者血清 lncRNA MALAT1水平降低，其可作为评价 CHF病情发展的潜在生物学标志物。
Abstract:
Objective　To explore the expression level of long non-coding RNA (lncRNA) MALAT1 in peripheral blood ofpatients with chronic heart failure (CHF) and its clinical significance. Methods　62 CHF patients admitted to the Department ofEmergency of Xi ’an Gaoxin Hospital from February 2017 to May 2019 were selected as the CHF group, and 70 healthy subjectsduring the same period were selected as the control group. The expression level of lncRNA MALAT1 in serum was detected byreal-time quantitative fluorescence PCR(qRT-PCR). The levels of apoptotic molecules in serum were determined by enzyme-linkedimmunosorbent assay (ELISA). Color Doppler ultrasonography was used to examine and determine the related indexes of heartfailure. The correlation was analyzed by Pearson correlation, receiver operating characteristic (ROC) curve was used to evaluatethe diagnostic efficacy of lncRNA MALAT1 for CHF. Results　The serum lncRNA malAT1 and B-cell lymphoma-2 (Bcl-2) levelsin CHF group were lower than those in the control group, and the serum Bcl2-associated X protein (Bax) levels in CHF group werehigher than those in the control group, the difference were statistically significant (t=16.31, 11.90, 9.57, all P=0.00). Patients withCHF group New York Heart Disease Assocation (NYHA) Ⅲ and Ⅳ serum lncRNA MALAT1 and Bcl-2 levels below theNYHA Ⅱ level, in CHF group NYHA Ⅲ and Ⅳ patients serum Bax levels higher than the NYHA level, NYHA Ⅳ Ⅱlevel in patients with serum lncRNA MALAT1 and Bcl-2 levels below Ⅲ level, the serum Bax level was higher than Ⅲ level,and the difference wars statistically significant (all P<0.05). The left ventricular ejection fraction (LVEF) in CHF group werelower than that in control group, the left ventricular end diastolic diameter (LVEDD) and left ventricular end systolic diameter(LVESD) were higher than those in the control group, the difference were statistically significant (t=4.56~10.85, all P=0.00).Pearson correlation analysis showed that serum lncRNA MALAT1 and Bcl-2 levels in CHF group were positively correlated withLVEF (r=0.29, 0.32, all P<0.05), while negatively correlated with LVEDD and LVESD (r=-0.25, -0.31, P=0.01). Serum Bax inCHF group was negatively correlated with LVEF (r=-0.33, P=0.01), while positively correlated with LVEDD and LVESD(r=0.20, 0.32, all P=0.00). In CHF group, serum lncRNA MALAT1 was positively correlated with the level of Bcl-2 (r=0.31,P=0.00), and negatively correlated with the level of serum Bax (r=-0.24, P=0.01). The area under the curve (AUC) of serumlncRNA MALAT1 in the diagnosis of CHF was 0.791, the sensitivity and the specificity were 75% and 79.5%,respectively(95%CI=0.6533~0.930, P=0.001). Conclusion　The serum lncRNA MALAT1 level was decreased in CHF patients, which canbe used as a potential biomarker to evaluate the disease progression of CHF.

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备注/Memo

备注/Memo:
作者简介：王新庄（1980-），男，主治医师，研究方向：急诊常见疾病的临床诊疗，E-mail:838035758@qq.com。
通讯作者：孟建涛（1987-），男，主治医师，研究方向：急诊常见疾病的临床诊疗，E-mail:369145939@qq.com。
更新日期/Last Update: 1900-01-01