[1]宋秀军,卢敬泰,牛冬梅,等.X射线照射对人外周血淋巴细胞 miRNA表达谱影响的初步研究[J].现代检验医学杂志,2022,37(01):159-163.[doi:10.3969/j.issn.1671-7414.2022.01.032]
　SONG Xiu-jun,LU Jing-tai,NIU Dong-mei,et al.Preliminary Study on miRNA Expression Profile of Human Peripheral Blood Lymphocytes Irradiated by X-ray[J].Journal of Modern Laboratory Medicine,2022,37(01):159-163.[doi:10.3969/j.issn.1671-7414.2022.01.032]
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X射线照射对人外周血淋巴细胞 miRNA表达谱影响的初步研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年01期
页码:
159-163
栏目:
研究简报 实验技术
出版日期:
2022-01-15

文章信息/Info

Title:
Preliminary Study on miRNA Expression Profile of Human Peripheral Blood Lymphocytes Irradiated by X-ray
文章编号:
1671-7414（2022）01-159-05
作者:
宋秀军1;  卢敬泰1; 牛冬梅2; 赵广利1; 吕　进1 ; 王　硕1 ; 李　娜1;  张阳东1
（1. 火箭军特色医学中心， 北京 100088；2. 火箭军96606 部队医院，河南洛阳 471003）
Author(s):
SONG Xiu-jun1;  LU Jing-tai1;  NIU Dong-mei2;  ZHAO Guang-li1;  L? Jin1; WANG Shuo1;  LI Na1;  ZHANG Yang-dong1
（1. PLA Rocket Force Characteristic Medical Center, Beijing 100088, China；2. 96606 Military Hospital of RocketArmy, Henan Luoyang 471003, China）
关键词:
X射线; 外周血淋巴细胞; miRNA; 生物标志物
分类号:
R818.74；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.01.032
文献标志码:
A
摘要:
目的　探讨 X射线照射人外周血淋巴细胞后 miRNA表达谱的变化，为寻找新的辐射损伤生物标志物奠定基础。方法　采集健康成年男性外周血，给予 2Gy与 4Gy X射线照射后分别于 12h和 24h分离外周血淋巴细胞，提取各组细胞总 RNA，采用 Taqman低密度流体芯片技术检测照射组与对照组的 miRNA表达谱差异，并选取 4种 miRNAs进行 PCR单管验证。结果　2Gy与 4Gy X射线照射人外周血淋巴细胞 12h可诱导 283种 miRNAs表达上调，473种 miRNAs表达下调； 2Gy与 4Gy X射线照射人外周血淋巴细胞 24h可诱导 464种 miRNAs表达上调，331种 miRNAs表达下调。其中， 2Gy与 4Gy X射线照射后，有 20种 miRNAs同时表现出时间反应性，即随着照射时间的延长而升高，其升高倍数为 0.25~27.43倍； 8种 miRNA随着照射时间的延长而降低，其下调倍数为 0.11~13.84倍。其次，照射 12h与 24h后，外周血淋巴细胞中仅发现 2种 miRNAs表现出剂量反应性，即随着照射剂量的升高而升高，其升高倍数为 0.16至 1.12倍； 24种 miRNAs随着剂量的增加而降低，其下调倍数为 0.08~45.34。结论　人外周血淋巴细胞经 2Gy与 4Gy X射线照射后，不同时间点可筛选出多种差异表达的 miRNAs，这些差异表达的 miRNAs表现出一定的时间和剂量反应性，可能是辐射损伤评估的潜在指标。
Abstract:
Objective　To investigate the changes of miRNA expression profile in human peripheral blood lymphocytes afterX-ray irradiation, and lay a foundation for finding new biomarkers of radiation injury. Methods　Peripheral blood samples werecollected from healthy adult males. After 2 Gy and 4 Gy X-ray irradiation, peripheral blood lymphocytes were isolated at 12hours and 24 hours, respectively. Total RNA was extracted from each group.TaqMan low density fluid chip technology was usedto detect the difference of miRNA expression profile between the irradiation group and the control group.4 kinds of miRNAswere selected for PCR single tube validation. Results　The expression of 283 miRNAs was up-regulated and 473 miRNAs wasdown regulated by 2Gy and 4Gy X-ray irradiation for 12 hours . The expression of 464 kinds of miRNAs were up-regulated and331 kinds of miRNAs were down regulated by 2Gy and 4Gy X-ray irradiation for 24 hours. Among them, 20 kinds of miRNAsshowed time responsiveness after 2Gy and 4Gy X-ray irradiation, that was, they increased with the extension of irradiation time,and the increase fold was 0.25 to 27.43. 8 miRNAs decreased with the prolongation of irradiation time, and the down-regulationfold ranged from 0.11 to 13.84. Secondly, only 2 kinds of miRNAs were found in peripheral blood lymphocytes after 12 hoursand 24 hours of irradiation, which showed dose-response, that was, they increased with the increase of irradiation dose, and theincrease fold was 0.16 to 1.12. 24 kinds of miRNAs decreased with the increase of dose, and the down-regulation fold was 0.08to 45.34. Conclusion　The changes of miRNA expression in peripheral blood were different at different time points after 2Gyand 4Gy X-ray irradiation. The differentially expressed miRNAs show a certain time and dose response, which may be a potentialindicator of radiation damage assessment.

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备注/Memo

备注/Memo:
基金项目：中国博士后科学基金面上项目（2014M552667）。
作者简介：宋秀军(1979-)，女，硕士，主管技师，主要从事放射生物学研究，E-mail:songxiujun1979@163.com。
通讯作者：张阳东(1970-)，男，博士，主任技师，主要从事基础医学研究，E-mail: zhangyd262@163.com。
更新日期/Last Update: 1900-01-01