[1]王淑明a,徐建男a,张建新b,等.维持性血液透析患者血清Beclin1和LC3-II表达水平与血管钙化的相关性分析[J].现代检验医学杂志,2022,37(02):132-136.[doi:10.3969/j.issn.1671-7414.2022.02.027]
　WANG Shu-minga,XU Jian-nana,ZHANG Jian-xinb,et al.Correlation between Serum Beclin1 and LC3-II Expression Levels and Vascular Calcification in Maintenance Hemodialysis Patients[J].Journal of Modern Laboratory Medicine,2022,37(02):132-136.[doi:10.3969/j.issn.1671-7414.2022.02.027]
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维持性血液透析患者血清Beclin1和LC3-II表达水平与血管钙化的相关性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年02期
页码:
132-136
栏目:
论　著
出版日期:
2022-03-15

文章信息/Info

Title:
Correlation between Serum Beclin1 and LC3-II Expression Levels and Vascular Calcification in Maintenance Hemodialysis Patients
文章编号:
1671-7414（2022）02-132-06
作者:
王淑明a; 徐建男a; 张建新b; 王月华a; 康京月a; 刘 畅a
（三河燕郊福合第一医院/ 原三河市燕郊人民医院a. 肾脏病内科；b. 急诊科，河北三河 065201）
Author(s):
WANG Shu-minga;  XU Jian-nana;  ZHANG Jian-xinb;  WANG Yue-huaa;  KANG Jing-yuea;  LIU Changa
(a.Department of Nephrology; b.Department of Emergency, the Yanjiao Fuhe First Hospital of Sanhe City/Yanjiao People’s Hospital of Sanhe City,Hebei Sanhe 065201,China)
关键词:
维持性血液透析; Beclin1; 微管相关蛋白1轻链3-II; 血管钙化
分类号:
R459.5；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.02.027
文献标志码:
A
摘要:
目的　探究维持性血液透析（maintenance hemodialysis，MHD）患者血清Beclin1 和微管相关蛋白1 轻链3-II（microtubule associated protein 1 light chain 3-II，LC3-II）表达水平与血管钙化（vascular calcification，VC）的相关性。方法　选取2017 年4 月～ 2020 年9 月三河市燕郊人民医院收治的MHD 患者145 例作为研究对象，根据是否发生VC，将患者分为MHD 未并发VC 组（n=75）和MHD 并发VC 组（n=70）。根据VC 评估结果将MHD 并发VC 患者分为轻度VC 组（n=36）、中度VC 组（n=24）、重度VC 组（n=10）；另选取同期在该院进行体检的健康人80 例作为对照组。收集受试者一般资料，酶联免疫吸附法（enzyme-linked immunosorbent assay，ELISA）检测受试者血清Beclin1 和LC3-II 的水平，Pearson 法分析MHD 患者血清Beclin1 和LC3-II 水平与VC 相关指标的相关性，二元logistic回归分析MHD 患者发生VC 的影响因素。结果　与对照组相比，MHD 未并发VC 组和MHD 并发VC 组血清肌酐（serum creatinine，SCr）（72.48±18.26μmol/L vs 685.30±192.42μmol/L ，733.98±206.35μmol/L）、血磷（1.28±0.42mmol/L vs 2.08±0.71mmol/L。2.86±0.87mmol/L）、血钙（1.54±0.45mmol/L vs 2.46±0.62mmol/L ，2.98±0.77mmol/L）、全段甲状旁腺激素（intact parathyroid hormone，iPTH）（58.10±17.36pg/ml vs 634.58±172.44pg/ml，769.48±195.02pg/ml）水平升高，差异有统计学意义（F=100.197 ～ 500.960，均P ＜ 0.05）；血清血红蛋白（hemoglobin，Hb）（134.82±35.16g/L vs 112.78±32.85g/L，103.66±27.91g/L），Beclin1（8.09±2.16μg/L vs 5.65±1.43μg/L，2.56±0.73μg/L）和LC3-II（45.16±5.15μg/L vs 36.31±3.42μg/L ，27.47±2.76μg/L）水平降低，差异有统计学意义（F=18.748 ～ 372.522，均P ＜ 0.05）；与MHD 未并发VC 组相比，MHD 并发VC 组血磷、血钙及iPTH 水平升高（t=7.136 ～ 9.723，均P＜ 0.05），血清Beclin1 和LC3-II 水平降低（t=16.605，18.982，均P ＜ 0.05）。轻度VC 组、中度VC 组和 重度VC组血清Beclin1（4.35±0.71μg/L，3.49±0.57μg/L 和1.91±0.26μg/L）和LC3-II（31.12±3.32μg/L，25.65±2.62μg/L vs 20.47±1.76μg/L）水平依次降低，差异均有统计学意义（F=366.298，296.025，均P ＜ 0.05）。MHD 患者血清Beclin1 水平与血磷、血钙及iPTH 均呈负相关（r=-0.674，-0.682，-0.597，均P ＜ 0.05）；血清LC3-II 水平与血磷、血钙及iPTH 也呈负相关（r=-0.648，-0.703，-0.674，均P ＜ 0.05）。二元logistic 回归分析发现，Beclin1 和LC3-II水平偏低是MHD 患者发生VC 的危险因素（P ＜ 0.05）。结论　MHD 并发VC 患者血清Beclin1 和LC3-II 水平降低，与VC 严重程度有关，是MHD 患者发生VC 的危险因素，可作为预测MHD 患者发生VC 的潜在生物学标志物。
Abstract:
Objective　To explore the correlation between the expression levels of serum Beclin1 and microtubule associated protein 1 light chain 3-II (LC3-II) and vascular calcification (VC) in maintenance hemodialysis (MHD) patients. Methods　A total of 145 MHD patients admitted to Yanjiao People’s Hospital of Sanhe City from April 2017 to September 2020 were selected as the research objects. According to the occurrence of VC, the patients were divided into MHD without VC group (n=75) and MHD with VC group (n=70). According to the results of VC evaluation, MHD patients with VC were divided into mild VC group (n=36), moderate VC group (n=24) and severe VC group (n=10). Another 80 healthy people were selected as thecontrol group.The general data of the subjects were collected, and the levels of serum Beclin1 and LC3-II were detected by enzyme-linked immunosorbent assay (ELISA). The correlation between serum Beclin1 and LC3-II levels and VC related indicators in MHD patients was analyzed by Pearson method. The influencing factors of VC in MHD patients were analyzed by binary logistic regression. Results　Compared with those in the control group, the levels of serum creatinine (SCr) (72.48±18.26μmol/L vs 685.30±192.42μmol/L, 733.98±206.35μmol/L), serum phosphorus (1.28±0.42mmol/L vs 2.08±0.71mmol/L ， 2.86±0.87mmol/L), calcium (1.54±0.45mmol/L vs 2.46±0.62mmol/L ，2.98±0.77mmol/L), intact parathyroid hormone (iPTH) (58.10±17.36pg/ml vs 634.58±172.44pg/ml ，769.48±195.02pg/ml) in MHD without VC group and MHD with VC group increased，the differences were statistically significant(F=100.197 ～ 500.960,all P ＜ 0.05), and the levels of hemoglobin (Hb) (134.82±35.16g/L vs 112.78±32.85g/L, 103.66±27.91g/L), Beclin1 (8.09±2.16μg/L vs 5.65±1.43μg/L, 2.56±0.73μg/L) and LC3-II (45.16±5.15μg/L vs 36.31±3.42μg/L, 27.47±2.76μg/L) in serum were decreased，the differences were statistically significant (F=18.748 ～ 372.522, all P ＜ 0.05). Compared with those in MHD without VC group, the levels of serum phosphorus, calcium and iPTH in MHD with VC group were higher (t=7.136 ～ 9.723, all P ＜ 0.05), and the levels of serum Beclin1 and LC3- II were lower (t=16.605, 18.982, all P ＜ 0.05). The levels of Beclin1 (4.35±0.71μg/L vs 3.49±0.57μg/L, 1.91±0.26μg/L) and LC3-II (31.12±3.32μg/L vs 25.65±2.62μg/L，20.47±1.76μg/L) in mild VC group, moderate VC group and severe VC group decreased in turn，the differences were statistically significant (F=366.298, 296.025，all P ＜ 0.05). The serum Beclin1 level was negatively correlated with serum phosphorus, calcium and iPTH in MHD patients (r=-0.674, -0.682，-0.597, all P ＜ 0.05). The level of serum LC3-II was negatively correlated with serum phosphorus, calcium and iPTH (r=-0.648, -0.703，-0.674, all P ＜ 0.05). Binary logistic regression analysis showed that low levels of Beclin1 and LC3-II were risk factors for VC in MHD patients (P ＜ 0.05). Conclusion　The levels of Beclin1 and LC3-II in MHD patients with VC were decreased, they were related to the severity of VC, which are risk factors for the occurrence of VC in MHD patients, and may be potential biomarker for predicting VC in MHD patients.

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备注/Memo

备注/Memo:
基金项目：河北省医学科学研究课题计划项目（编号20200115）；廊坊市科学技术研究与发展计划项目（编号2018013135）。
作者简介：王淑明（1979-），女，本科，主治医师，研究方向：维持性血液透析患者并发症的治疗，E-mail：shumingwang795@163.com。
更新日期/Last Update: 1900-01-01