[1]王庆妹,崔慧娟,杜京辉,等.外周血单个核细胞miRNA-492和miRNA-155水平表达与SNAPPE-Ⅱ评分对儿童急性呼吸窘迫综合征预后价值研究[J].现代检验医学杂志,2022,37(03):6-10.[doi:10.3969/j.issn.1671-7414.2022.03.002]
 WANG Qing-mei,CUI Hui-juan,DU Jing-hui,et al.Study on the Prognostic Value of miRNA-492 and miRNA-155 Levels in Peripheral Blood Mononuclear Cells and SNAPPE- Ⅱ Score in Children with Acute Respiratory Distress Syndrome[J].Journal of Modern Laboratory Medicine,2022,37(03):6-10.[doi:10.3969/j.issn.1671-7414.2022.03.002]
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外周血单个核细胞miRNA-492和miRNA-155水平表达与SNAPPE-Ⅱ评分对儿童急性呼吸窘迫综合征预后价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年03期
页码:
6-10
栏目:
论 著
出版日期:
2022-05-15

文章信息/Info

Title:
Study on the Prognostic Value of miRNA-492 and miRNA-155 Levels in Peripheral Blood Mononuclear Cells and SNAPPE- Ⅱ Score in Children with Acute Respiratory Distress Syndrome
文章编号:
1671-7414(2022)03-006-06
作者:
王庆妹1崔慧娟1杜京辉1庄元华2
(1. 兖矿新里程总医院,山东邹城 273500;2 . 山东大学齐鲁儿童医院,济南 250021)
Author(s):
WANG Qing-mei1 CUI Hui-juan1 DU Jing-hui1 ZHUANG Yuan-hua2
(1. Yankuang New Milestone General Hospital, Shandong Zoucheng 273500,China;2 .Qilu Children’s Hospital of Shandong University, Jinan 250021, China)
关键词:
急性呼吸窘迫综合征微小核糖核酸-492微小核糖核酸-155急性生理学评分围产期补充- Ⅱ评分
分类号:
R725.6;R392.11
DOI:
10.3969/j.issn.1671-7414.2022.03.002
文献标志码:
A
摘要:
目的 探究外周血单个核细胞 miR-492和 miR-155表达水平与新生儿急性生理学评分围产期补充Ⅱ评分( score for neonatal acute physiology perinatal extension,SNAPPE-Ⅱ)评价儿童急性呼吸窘迫综合征( acute respiratory distress syndrome,ARDS)预后的价值。方法 选取 2019年 9月~ 2020年 11月兖矿新里程总医院及山东大学齐鲁儿童医院收治的 ARDS患儿 70例,分为死亡组( n=18)和存活组( n=52),同期健康体检儿童 36例为对照组。比较三组 miR492,miR-155,氧合指数( PaO2/FiO2),SNAPPE-Ⅱ评分及急性生理与慢性健康( acute physiology and chronic health evaluation Ⅱ,APACHEⅡ)评分。分析 3个指标与 PaO2/FiO2和 APACHEⅡ评分间的相关性及预测死亡的价值。结果 三组儿童 PaO2/FiO2(448.64±85.54, 269.64±31.53, 165.64±27.63 mmHg),APACHEⅡ评分 (5.74±1.42, 20.54±4.29, 29.65±5.43分 )、SNAPPE-Ⅱ评分( 5.95±1.75,15.86±3.75, 36.45±5.32分)、 miR-492(3.75±0.98, 5.63±1.41, 9.54±2.25)和 miR-155(1.75±0.95, 3.84±0.53, 5.64±1.74)水平比较,差异均有统计学意义(均 P< 0.05)。ARDS 存活组与 ARDS死亡组患儿 PaO2/FiO2,APACHEⅡ评分、SNAPPE-Ⅱ评分、miR-492, miR-155水平比较,差异均有统计学意义( t =9.245~16.546, 均 P<0.001)。PaO2/FiO2与 miR-492,miR-155和 SNAPPE-Ⅱ评分呈负相关(r =-0.584, -0.623, -0.594, 均 P< 0.05);APACHEⅡ评分与 miR-492,miR-155和 SNAPPE-Ⅱ评分呈正相关( r =0.863, 0.868, 0.757,均 P< 0.05)。三指标联合预测死亡诊断效能高于单一指标,临界值 miR-492为 0.84,miR-155为 1.43,SNAPPE-Ⅱ评分为 22.8。结论 miR-492,miR-155和 SNAPPE-Ⅱ评分联合检测能够有效预测 ARDS患儿预后,当 miR-492> 0.84,miR-155> 1.43和 SNAPPE-Ⅱ评分> 22.8分时,ARDS患儿的死亡率较高。
Abstract:
Objective To explore the value of miR-492 and miR-155 expression levels and neonatal acute score for neonatal acute physiology perinatal supplement Ⅱ score (SNAPPE- Ⅱ ) in evaluating the prognosis of children with acute respiratory distress syndrome (ARDS). Methods 70 children with ARDS admitted to Yankuang New Milestone General Hospital and Qilu Children’s Hospital of Shandong University from September 2019 to November 2020 were divided into death group (n=18) and survival group (n=52). During the same period, 36 children under physical examination served as the control group. The three groups of miR-492, miR-155, PaO2/FiO2, SNAPE- Ⅱ score and APACHE Ⅱ score were compared, and analyzed the correlation between the three indicators and PaO2/FiO2, APACHE Ⅱ scores and the value of predicting death. Results PaO2/ FiO2(448.64±85.54, 269.64±31.53, 165.64±27.63 mmHg),APACHE Ⅱ (5.74±1.42,20.54±4.29,29.65±5.43),SNAP PE- Ⅱ (5.95±1.75,15.86±3.75,36.45±5.32),miR-492(3.75±0.98,5.63±1.41,9.54±2.25) and miR-155(1.75±0.95, 3.84±0.53, 5.64±1.74) in the three groups, the differences were statistically significant (all P < 0.05). ARDS survival group and death group PaO2/FiO2,APACHE Ⅱ scores, SNAPPE- Ⅱ scores, miR-492, miR-155 levels the differences were statistically significant (t =9.245~16.546, all P<0.001).PaO2/FiO2 was negatively correlated with miR-492, miR-155 and SNAPPE- Ⅱ scores (r =-0.584, -0.623, -0.594, all P < 0.05). APACHE Ⅱ score was positively correlated with miR-492, miR-155 and SNAPPE- Ⅱ scores(r =0.863, 0.868, 0.757, all P < 0.05). The diagnostic performance of the three indicators combined to predict death was higher than that of a single indicator, the critical value of miR-492 was 0.84, miR-155 was 1.43, SNAPPE- Ⅱ score was 22.8. Conclusion The combination of miR-492, miR-155 and SNAPPE- Ⅱ scores can effectively predict the prognosis of children with ARDS. When miR-492 > 0.84, miR-155 > 1.43 and SNAPPE- Ⅱ score > 22.8, the mortality rate of children with ARDS higher.

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备注/Memo

备注/Memo:
作者简介:王庆妹(1978-),女,硕士,主治医师,研究方向:儿科学,E-mail:b475214523@163.com。
更新日期/Last Update: 1900-01-01