[1]游铠旭,魏　星,徐　革.青霉素结合蛋白3编码基因ftsI在多重耐药鲍曼不动杆菌中表达及碳青霉烯类诱导对其表达的影响[J].现代检验医学杂志,2022,37(03):47-52.[doi:10.3969/j.issn.1671-7414.2022.03.010]
　YOU Kai-xu,WEI Xing,XU Ge.Expression of Penicillinbinding Protein 3 Encoding Gene FtsI in Multidrug-resistant Acinetobacter Baumannii and the Effect of Carbapenems Induction on Its Expression[J].Journal of Modern Laboratory Medicine,2022,37(03):47-52.[doi:10.3969/j.issn.1671-7414.2022.03.010]
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青霉素结合蛋白3编码基因ftsI在多重耐药鲍曼不动杆菌中表达及碳青霉烯类诱导对其表达的影响()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年03期
页码:
47-52
栏目:
论　著
出版日期:
2022-05-15

文章信息/Info

Title:
Expression of Penicillinbinding Protein 3 Encoding Gene FtsI in Multidrug-resistant Acinetobacter Baumannii and the Effect of Carbapenems Induction on Its Expression
文章编号:
1671-7414（2022）03-047-06
作者:
游铠旭; 魏　星; 徐　革
（成都医学院第三附属医院/ 成都市郫都区人民医院检验科, 成都 611730）
Author(s):
YOU Kai-xu; WEI Xing; XU Ge
(Department of Clinical Laboratory, the Third Affiliated Hospital of Chengdu Medical College/Pidu District People’s Hospital of Chengdu, Chengdu 611730，China)
关键词:
鲍曼不动杆菌; 多重耐药; ftsI
分类号:
R378.2；Q786
DOI:
10.3969/j.issn.1671-7414.2022.03.010
文献标志码:
A
摘要:
目的　研究青霉素结合蛋白 3（penicillin-binding protein 3，PBP3）编码基因 ftsI在多重耐药鲍曼不动杆菌（Multi drug resistant Acinetobacter baumannii，MDR-AB）中表达情况，探明碳氢霉烯诱导对 MDR-AB中 ftsI表达的影响。方法　收集 2019年 1月～ 6月成都市郫都区人民医院临床分离的 38株 MDR-AB菌株， PCR扩增 ftsI基因，产物进行测序，将 MDR-AB菌株与鲍曼不动杆菌敏感株（ SDF）ftsI基因序列翻译成氨基酸序列进行同源建模和可视化比对分析。然后再分别用亚胺培南、美罗培南和多尼培南对菌株进行诱导， qRT-PCR检测 ftsI的表达情况。结果　38株 MDR-AB均携带 ftsI基因，测序结果与 Genbank 中 ATCC 17978 标准菌株 ftsI 序列显示 99.54% 同源，将菌株 MDR-AB 1与 SDF株的 ftsI基因序列翻译成氨基酸序列后，发现 SDF菌株多出 251号位赖氨酸、252号位苏氨酸、253号位甘氨酸和 254号位缬氨酸，同源建模和可视化分析发现两者 ftsI基因编码蛋白空间结构明显不同。MDR-AB菌株经三种碳氢霉烯类抗生素诱导后， ftsI基因相对表达量均下降，其中亚胺培南组和美罗培南组相对于未处理组 ftsI基因表达下调差异有统计学意义（ t=5.314, 5.424, 均 P＜ 0.05）。结论　MDR-AB普遍携带 ftsI基因，其 ftsI编码蛋白空间结构与 SDF菌株存在明显差异，这可能是 AB产生耐药的一个重要因素。碳氢霉烯类诱导可导致 ftsI基因表达下调，以亚胺培南、美罗培南诱导效果最明显。
Abstract:
Objective　To investigate the gene ftsI encoding penicillin binding protein 3 (PBP3) in Multi drug resistant Acinetobacter baumannii(MDRAB), and observe the expression of ftsI in MDR-AB and the effect of carbasolene induction on the expression of ftsI. Methods　The 38 strains of MDR-AB isolated from Pidu District People’s Hospital of Chengdu from January to June 2019 , and ftsI gene was amplified by PCR and the products were sequenced and analyzed. The ftsI gene sequences of MDR-AB strain and Acinetobacter baumannii sensitive strain (SDF) were translated into amino acid sequences for homology modeling and visual comparison. Then, the strains were induced with Imipenem,Meropenem and Donipenem, and the expression of ftsI was detected by qRT-PCR. Results　All the 38 strains of MDR-AB carried ftsI gene,and the sequencing results showed 99.54% homology with the ftsI sequence of ATCC 17978 standard strain in GenBank.After translating the ftsI gene sequences of MDR-AB 1 and SDF strains into amino acid sequences, it was found that SDF strains had more Lys251, Thr252, Gly253 and Val254. Homology modeling and visualization analysis showed that the spatial structure of the proteins encoded by ftsI gene was significantly different between the two strains. The relative expression of ftsI gene in the MDR-AB strain was decreased after induction by three kinds of carbasenes, and the Imipenem group and Meropenem group was statistically different from that in the untreated group (P ＜ 0.05). Conclusion　MDR-AB generally carries ftsI gene, and the spatial structure of ftsI encoding protein was significantly different from that of SDF strain, this may be an important factor in the resistance of AB.Carbonasene induction can lead to the down-regulation of ftsI gene expression, and the induction of Imipenem and Meropenem is most obvious.

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备注/Memo

备注/Memo:
基金项目： 四川省卫计委基金资助项目（18PJ108），四川省基层卫生事业发展研究中心2020 年立项项目(SWFZ20-C-070)，成都医学院校级课题（CYZYB20-23)。
作者简介：游铠旭（1978-），男，本科，副主任技师，主要研究方向：病原微生物，E-mail：549517576@qq.com。
通讯作者：徐革（1967-），男，本科，主任技师，主要研究方向：临床检验诊断学，E-mail：xuge1967@163.com。
更新日期/Last Update: 1900-01-01