[1]康 茹,成晓悦,王 琪,等.化学发光免疫法分析孕妇血清甲状腺球蛋白抗体浓度分布及其对甲状腺球蛋白水平检测的影响研究[J].现代检验医学杂志,2023,38(01):165-168+178.[doi:10.3969/j.issn.1671-7414.2023.01.031]
　KANG Ru,CHENG Xiao-yue,WANG Qi,et al.Study on the Distribution of Serum Thyroglobulin Antibody in Pregnant Women by Chemiluminescence Immunoassay and Its Interference on the Detection of Thyroglobulin[J].Journal of Modern Laboratory Medicine,2023,38(01):165-168+178.[doi:10.3969/j.issn.1671-7414.2023.01.031]
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化学发光免疫法分析孕妇血清甲状腺球蛋白抗体浓度分布及其对甲状腺球蛋白水平检测的影响研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年01期
页码:
165-168+178
栏目:
研究简报·实验技术
出版日期:
2023-01-15

文章信息/Info

Title:
Study on the Distribution of Serum Thyroglobulin Antibody in Pregnant Women by Chemiluminescence Immunoassay and Its Interference on the Detection of Thyroglobulin
文章编号:
1671-7414（2023）01-165-05
作者:
康 茹1; 成晓悦1; 王 琪2; 谢 云1; 杨军兰1; 孟改利1
（1. 西北妇女儿童医院医学检验中心，西安710061；2. 西安交通大学第二附属医院检验科，西安710002）
Author(s):
KANG Ru1;  CHENG Xiao-yue1;  WANG Qi2;  XIE Yun1;  YANG Jun-lan1;  MENG Gai-li1
(1. Department of Clinical Laboratory, Northwest Women’s and Children’s Hospital，Xi’an 710061, China; 2. Department of Clinical Laboratory, the Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710002, China)
关键词:
孕妇; 甲状腺球蛋白抗体; 甲状腺球蛋白
分类号:
R714.147；Q503
DOI:
10.3969/j.issn.1671-7414.2023.01.031
文献标志码:
A
摘要:
目的 分析孕妇人群中甲状腺球蛋白抗体（thyroglobulin antibody，TgAb）的阳性率以及其体内不同浓度的TgAb 对甲状腺球蛋白（thyroglobulin，Tg）检测的影响。方法 回顾性分析2020 年10 月~2021 年12 月在西北妇女儿童医院门诊孕检的68 955 例孕妇血清TgAb 和Tg 的结果，并收集240 份不同浓度TgAb 的孕妇血清，进行Tg 确证血清回收实验，分析不同浓度TgAb 对Tg 检测的影响。结果 依据西门子检测系统的阳性结果判读标准（TgAb ≥ 60IU/ml），68 955 例孕妇TgAb 阳性率为15.47%（10 665/68 955），且TgAb 阳性率随着孕妇年龄增长呈增高趋势（χ2=101.931，P<0.001）。按照TgAb（IU/ml）<60（阴性），60~200，201~300，301~400，401~500 和>500 进行分组，其对应的Tg检测浓度（ng/ml）范围分别为11.26（2.87~43.80），3.40（0.13~63.95），3.15（0.15~70.70），0.60（0.13~50.92），0.59（0.14~64.22）和0.74（0.14~14.82）。Tg 检测浓度与TgAb 水平呈负相关（r=-0.504，P<0.001）。按照上述分组，其对应的Tg 确证血清回收率分别为102.10%（81.09%~106.05%），97.03%（43.15%~109.29%），95.57%（41.67%~106.20%），84.57%（33.41%~105.25%），66.97%（29.33%~104.50%）和65.98%（21.70%~98.56%）。Tg 确证血清回收率与TgAb水平呈负相关（r=-0.565，P<0.001）。Tg 确证血清回收率小于80% 的占比分别为0%（0/40），27.5%（11/40），35%（14/40），42.5%（17/40），62.5%（25/40）和67.5%（27/40）。孕妇不同TgAb 浓度血清的Tg 确证血清回收率小于80% 的百分率差异有统计学意义（χ2=51.134，P<0.001），且随着TgAb 浓度的增高，呈增高趋势（χ2=48.363，P<0.001）。结论 当孕妇体内TgAb 为阴性时，Tg 的检测结果无影响；当TgAb 阳性时，会干扰Tg 检测，使其结果偏低，且浓度越高对Tg 的干扰越强。由于孕妇群体TgAb 阳性率较高，建议临床进行Tg 检测时同时联合检测TgAb 综合判断。
Abstract:
Objective To analyze the positive rate of thyroglobulin antibody (TgAb) in pregnant women and investigate the interference of different concentrations of TgAb in pregnant women on the detection of thyroglobulin (Tg). Methods The results of TgAb positive rate of 68 955 pregnant women with pregnancy examination in Northwest Women’s and Children’s Hospital were analyzed retrospectively, from October 2020 to December 2021. 240 samples of TgAb serum of pregnant women with different concentration levels were collected, and Tg confirmatory serum was added for recovery experiment. Results According to the result interpretation standard of Siemens detection system, the positive rate of TgAb (TgAb ≥ 60IU/ml) in pregnant women was 15.47% (10 665/68 955), increasing with the age of pregnant women (χ2= 101.931, P < 0.001). TgAb (IU/ ml) concentrations were grouped according to <60(negative), 60~200, 201~300, 301~400, 401~500 and >500 .Corresponding to different groups, Tg detection concentration ranges(ng/ml) were 11.26 (2.87~43.80), 3.40 (0.13~63.95), 3.15 (0.15~70.70), 0.60 (0.13~50.92), 0.59 (0.14~64.22) and 0.74 (0.14~14.82), and there was a significant negative correlation between the concentration of Tg and the level of TgAb, and the correlation coefficient was r=-0.504 (P<0.001).The recovery rate of different TgAb concentrations of maternal serum was statistically different (P<0.001). According to the above grouping, the corresponding recovery rates of Tg confirmatory serum were 102.10% (81.09%~106.05%), 97.03% (43.15%~109.29%), 95.57% (41.67%~106.20%), 84.57% (33.41%~105.25%), 66.97% (29.33%~104.50%) and 65.98% (21.70%~98.56%)，and Tg confirmed recovery rate was significantly negatively correlated with the level of TgAb, and the correlation coefficient was r=-0.565, P<0.001.The average recoveries less than 80% confirmed by Tg were 0% (0/40), 27.5% (11/40), 35% (14/40), 42.5% (17/40), 62.5% (25/40) and 67.5% (27/40), respectively. The percentage of recovery less than 80% of different concentrations of TgAb in maternal serum was statistically different (χ2=51.134, P<0.001), and showed an increasing trend with the increase of TgAb concentration (χ2=48.363, P<0.001). Conclusion When TgAb in pregnant women was negative, the detection results of Tg would be unaffected. When TgAb was positive, the higher the concentration, the stronger the interference to Tg, making the lower result. Due to the high positive rate of TgAb in pregnant women, it is recommended to jointly detect TgAb for comprehensive judgment during Tg testing.

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备注/Memo

备注/Memo:
作者简介：康茹（1989-），女，硕士研究生，检验技师，主要从事感染与免疫的研究，E-mail：kangkang123kk@126.com。
通讯作者：孟改利（1986-），女，硕士研究生，主管技师，主要从事感染与免疫的研究，E-mail：mgl170618@sina.com。
更新日期/Last Update: 2023-01-15