[1]张莹莹a,梁 琛b,李晓燕b,等.盆底功能障碍性疾病患者血清miR-4429 和miR-19-3p 水平表达及临床价值研究[J].现代检验医学杂志,2024,39(02):119-123.[doi:10.3969/j.issn.1671-7414.2024.02.022]
　ZHANG Yingyinga,LIANG Chenb,LI Xiaoyanb,et al.Expression and Clinical Value of Serum miR-4429 and miR-19-3p in Patients with Pelvic Floor Dysfunctional Disease[J].Journal of Modern Laboratory Medicine,2024,39(02):119-123.[doi:10.3969/j.issn.1671-7414.2024.02.022]
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盆底功能障碍性疾病患者血清miR-4429 和miR-19-3p 水平表达及临床价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年02期
页码:
119-123
栏目:
论著
出版日期:
2024-03-31

文章信息/Info

Title:
Expression and Clinical Value of Serum miR-4429 and miR-19-3p in Patients with Pelvic Floor Dysfunctional Disease
文章编号:
1671-7414（2024）02-119-05
作者:
张莹莹a; 梁 琛b; 李晓燕b; 张艳玲b; 酒卫莉b
（衡水市第二人民医院a. 神经泌尿外科；b. 产科，河北衡水 053000）
Author(s):
ZHANG Yingyinga;  LIANG Chenb;  LI Xiaoyanb;  ZHANG Yanlingb;  JIU Weilib
(a.Department of Neurourology；b.Obstetrics and Gynecology, the Second People’s Hospital of Hengshui, Hebei Hengshui 053000, China)
关键词:
盆底功能障碍性疾病; 微小RNA-4429; 微小RNA-19-3p
分类号:
R711.5；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.02.022
文献标志码:
A
摘要:
目的 探究盆底功能障碍性疾病（pelvic floor dysfunctional disease，PFD）患者血清微小RNA（microRNA,miR）-4429 和微小RNA（microRNA，miR）-19-3p 水平表达及意义。方法 选取2021 年6 月~2022 年6 月衡水市第二人民医院收治的PFD 患者90 例作为PFD 组，分为盆腔器官脱垂（pelvic organ prolapse，POP）组（n=50）、压力性尿失禁（stress urinary incontinence，SUI）组（n=25）和POP 并发SUI 组（n=15）；并选取同期在衡水市第二人民医院检查的健康女性80 例为对照组。比较对照组与PFD 组分娩方式、既往流产史、PFD 家族史等一般资料。比较各组血清miR-4429 和miR-19-3p 水平。受试者工作特征（receiver operating characteristic，ROC）曲线分析血清miR-4429，miR-19-3p 水平对PFD 的诊断价值。Logistic 回归分析影响PFD 的因素。配对样本t 检验比较PFD 治疗前后血清miR-4429，miR-19-3p 水平变化。结果 PFD 组在分娩方式、既往流产史、PFD 家族史方面与对照组相比差异均有统计学意义（t=4.415，6.444，4.707，均P ＜ 0.05）。PFD 组血清miR-4429 水平较对照组降低（0.71 ± 0.19 vs 1.00 ± 0.25），miR-19-3p 水平较对照组升高（1.44 ± 0.35 vs 1.01 ± 0.28），差异具有统计学意义（t=8.927，8.772，均P ＜ 0.05）。POP 组和SUI 组血清miR-4429 水平高于POP 并发SUI 组（0.73 ± 0.22，0.74 ± 0.16 vs 0.59 ± 0.16），POP 组和SUI组血清miR-19-3p 水平低于POP 并发SUI 组（1.35 ± 0.39 ， 1.41 ± 0.31 vs 1.77 ± 0.56），差异具有统计学意义（t=3.531，3.411；5.003，3.865，均P ＜ 0.05）。ROC 曲线分析显示，miR-4429，miR-19-3p 可辅助评估是否发生PFD 的曲线下面积（area under curve，AUC）分别为0.805，0.825，二者联合检测的AUC 为0.865。多因素分析显示，miR-19-3p 是影响PFD 的危险因素，miR-4429 是保护因素。经过治疗发现，PFD 患者血清miR-4429 水平升高（0.93 ± 0.23 vs 0.71± 0.19），miR-19-3p 水平降低（1.12 ± 0.29 vs 1.44 ± 0.35），差异具有统计学意义（t=6.996，6.679，均P ＜ 0.05）。结论 PFD 患者血清miR-4429 水平降低，miR-19-3p 水平升高，血清miR-4429，miR-19-3p 水平与PFD 疾病的发生发展密切相关，可作为预测PFD 的评估指标。
Abstract:
Objective To investigate the expression and significance of microRNA (miR) -4429 and microRNA (miR) -19-3p level in patients with pelvic floor dysfunctional (PFD)disease. Methods A total of 90 PFD patients admitted to the Second People’s Hospital of Hengshui from June 2021 to June 2022 were selected as the PFD group. They were grouped into the pelvic organ prolapse (POP) group（n=50）, the stress urinary incontinence (SUI) group（n=25）, and the POP combined with SUI group（n=15）.Meanwhile, 80 healthy women who were examined in the Second People’s Hospital of Hengshui were collected as the control group. The general data such as delivery mode, previous abortion history and family history were compared between the control group and the PFD group. The levels of serum miR-4429 and miR-19-3p in each group were compared. The diagnostic value of serum miR-4429 and miR-19-3p levels in PFD was analyzed by receiver operating characteristic (ROC) analysis. Logistic regression analysis was applied to analyze the factors affecting PFD. The paired sample t-test was applied to compare the changes in serum miR-4429 and miR-19-3p levels before and after PFD treatment. Results There were significant differences between the PFD group and the control group in terms of delivery mode, previous abortion history, and PFD family history, and the differences were statistically significant (t=4.415, 6.444, 4.707, all P ＜ 0.05). The serum miR-4429 (0.71±0.19 vs 1.00± 0.25) level in the PFD group was lower than that in the control group, while the miR-19-3p (1.44±0.35 vs 1.01± 0.28) level was higher than that in the control group, and the differences was statistically significant (t=8.927, 8.772, all P ＜ 0.05). The serum miR-4429 (0.73 ± 0.22，0.74 ± 0.16 vs 0.59 ± 0.16) level in the POP and SUI groups was higher than that in the POP combined SUI group, while the serum miR-19-3p (1.35 ± 0.39，1.41 ± 0.31 vs 1.77 ± 0.56) level in the POP group and SUI group was lower than that in the POP combined with SUI group, with significant differences (t=3.531, 3.411; 5.003, 3.865, all P ＜ 0.05). ROC analysis showed that the areas under curve (AUC) for miR-4429 and miR-19-3p to assist in assessing whether PFD occurs were 0.805 and 0.825, respectively. The AUC of the combined detection was 0.865. Multivariate logistic regression analysis showed that miR-19-3p was a risk factor affecting PFD, while miR-4429 was a protective factor. After treatment, serum miR-4429 (0.93 ± 0.23 vs 0.71 ± 0.19) level in PFD patients increased, while the miR-19-3p (1.12 ± 0.29 vs 1.44 ± 0.35) level decreased, the diffences were statistically significant (t=6.996, 6.679, all P ＜ 0.05). Conclusion The serum miR-4429 level in patients with PFD decreased, while the miR-19-3p level increased. The levels of miR-4429 and miR-19-3p in serum were closely related to the occurrence and development of PFD diseases, which can be used as evaluation indicators to predict PFD.

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备注/Memo

备注/Memo:
基金项目：衡水市科技计划项目任务书（2019014015Z）：不同分娩方式对产妇盆底功能障碍性疾病的影响和康复训练的临床研究。
作者简介：张莹莹（1984-），女，本科，副主任医师，研究方向：女性泌尿系统疾病，E-mail：zyy1984z@163.com。
通讯作者：梁琛（1983-），女，硕士研究生，副主任医师，研究方向：妇产科学，E-mail：xieqiap7@163.com。
更新日期/Last Update: 2024-03-15