[1]胡 娟,薛怀裕,呼永河.高湿地区不同海拔人群鼻腔nuc-mecA基因定植研究[J].现代检验医学杂志,2015,30(01):11-14.[doi:10.3969/j.issn.1671-7414.2015.01.004]
　HU Juan,XUE Huai-yu,HU Yong-he.Differences of Nuc-mecA Gene Carriage in the Noses among Different Altitudes in High Humidity District[J].Journal of Modern Laboratory Medicine,2015,30(01):11-14.[doi:10.3969/j.issn.1671-7414.2015.01.004]
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高湿地区不同海拔人群鼻腔nuc-mecA基因定植研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年01期
页码:
11-14
栏目:
论著
出版日期:
2015-01-30

文章信息/Info

Title:
Differences of Nuc-mecA Gene Carriage in the Noses among Different Altitudes in High Humidity District
文章编号:
1671-7414(2015)01-011-04
作者:
胡 娟1; 薛怀裕1; 呼永河2
1.成都军区临床医学检验中心,成都 610083;
2.成都军区总医院,成都 610083
Author(s):
HU Juan1; XUE Huai-yu1; HU Yong-he2
1.Clinical Medical Inspection Center,Chengdu Military Region of PLA,Chengdu 610083,China;
2.General Hospital of Chengdu Military Region of PLA,Chengdu 610083,China
关键词:
高湿;  高原;  nuc-mecA基因;  定植;  实时荧光PCR技术
分类号:
Q503; Q786
DOI:
10.3969/j.issn.1671-7414.2015.01.004
文献标志码:
A
摘要:
目的 调查高湿地区不同海拔高度世居人群鼻腔内nuc-mecA基因定植情况,为高原高湿地区金黄色葡萄球菌及甲氧西林耐药病原菌的防治和抗生素的合理使用提供数据。方法 收集1 000 m,1 200 m和1 400 m三个不同海拔高度人群鼻拭子样本,采用多通道实时荧光PCR技术检测其鼻腔nuc-mecA基因,比较不同海拔高度人群鼻腔nuc基因及mecA基因差异。结果 1 000 m,1200 m和1 400 m三个不同海拔高度地区人群鼻腔内nuc基因定植率分别为4.878%,2.899%和7.143%,差异无统计学意义(P均>0.05)。1 000 m,1 200m和1 400 m三个海拔高度地区人群鼻腔mecA基因定植率分别为:14.634%,31.884%和41.837%。海拔1 000 m人群与海拔1 200 m人群相比,其鼻腔内mecA基因定植率差异有统计学意义(P<0.05); 海拔1 000 m人群与海拔1 400 m人群相比,其鼻腔内mecA基因定植率差异有统计学显著性意义(P<0.01)。1 000 m,1 200 m和1 400 m三个海拔高度地区人群鼻腔nuc-mecA基因定植率分别为:0%,1.449%和3.061%,差异无统计学意义(P均>0.05)。结论随着海拔的升高,耐甲氧西林mecA基因在鼻腔内的定植显著增多。较高海拔地区居民应注意保持鼻腔等病原菌易定植部位的清洁,患病后应及时去医院就诊,不擅自使用抗生素; 医务人员应合理谨慎使用抗菌药物,避免抗生素的滥用和过度医疗。
Abstract:
Objective To investigate the carriage of nuc-mecA gene among different altitudes in high humidity district,provided guiding data for prevention of staphylococcus aureus and drug-resistant bacteria,standardizing the usage for antibiotics.Methods The nose swabswere collected in different altitudes:1 000 m,1 200 m and 1 400 m,nuc-mecAgene was confirmed by multi-channel real-time PCR.Results The carrier of nuc gene in the noses were 4.878%,2.899% and 7.143%,in 1000 m,1 200 m and 1 400 m respectively,and there were no statistical significant among the altitudes(P>0.05).The carrier of mecA gene were 14.634%,31.884% and 41.837% in the 1 000 m,1 200 m and 1 400 m respectively,the difference showed statistical significe(P<0.05).The carrying rate of mecA geneshowed statistically significant differences between 1 000 m and 1 200 m(P<0.05),and showed statistically significant differences between 1 000 m and 1400 m(P<0.01)also.The carrier of nuc-mecA gene were 0%,1.449% and 3.061% in 1 000 m,1 200 m and 1 400 m respectively,there were no statistical significant(P>0.05).Conclusion The carrier of mecA gene in noses was increased with the increasing of the altitude.The residents wholiving at higher altitude should keep the colonization sites of pathogens clean,and needed timely medical when got sick,shouldn't abuse the antibiotics without authorization.Medical staff should rational use of antibiotic drugs,avoided overusing of antibiotics and overtreatment.

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备注/Memo

备注/Memo:
基金项目:美国医学研究基金(亚洲区)临床微生物学研究基金(CNSC-J2011-A330),全军医学科技“十二五”重点项目(BWS11J067)。
作者简介:胡 娟(1959-),女,博士,主任技师,从事临床检验医学和流行病学研究工作,Tel:028-86571260,E-mail:13880731591@163.com。
更新日期/Last Update: 2015-01-30