[1]多丽波,李桂玲,陈淑娟,等.铜绿假单胞菌金属酶及整合酶的检测[J].现代检验医学杂志,2015,30(01):34-38.[doi:10.3969/j.issn.1671-7414.2015.01.010]
　DUO Li-bo,LI Gui-ling,CHEN Shu-Juan,et al.Detection of Metallo-β-Lactamases and Integrasesin from Pseudomonas Aeruginosa Isolates[J].Journal of Modern Laboratory Medicine,2015,30(01):34-38.[doi:10.3969/j.issn.1671-7414.2015.01.010]
点击复制

铜绿假单胞菌金属酶及整合酶的检测()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年01期
页码:
34-38
栏目:
论著
出版日期:
2015-01-30

文章信息/Info

Title:
Detection of Metallo-β-Lactamases and Integrasesin from Pseudomonas Aeruginosa Isolates
文章编号:
1671-7414(2015)01-034-05
作者:
多丽波1; 李桂玲1; 陈淑娟1; 栾 英1; 王兴力2
1.哈尔滨医科大学附属第二医院检验科, 哈尔滨 150086;
2.黑河市第一人民医院检验科,黑龙江黑河 164000
Author(s):
DUO Li-bo1; LI Gui-ling1; CHEN Shu-Juan1; LUANYing1; WANG Xing-li2
1.Department of Clinical Laboratory,the Second Affiliated Hospital of Harbin Medical University,Harbin 150086,China;
2.Department of Clinical Laboratory, the First People's Hospital of Heihe City,Heilongjiang Heihe 164000,China
关键词:
铜绿假单胞菌;  金属酶;  整合酶
分类号:
R378.991; Q781
DOI:
10.3969/j.issn.1671-7414.2015.01.010
文献标志码:
A
摘要:
目的 了解哈尔滨医科大学附属第二医院铜绿假单胞菌(Pa)株中金属β-内酰胺酶及整合酶携带情况。方法 采用改良Hodge法、双纸片协同法、组合纸片法进行金属酶表型检测,PCR法检测金属酶基因型以及整合酶Ⅰ、整合酶Ⅱ和整合酶Ⅲ。将IMP金属酶全基因PCR产物进行纯化,测序再进行Blast比对分析。结果 62株Pa经金属β-内酰胺酶改良Hodge法检出3株、双纸片协同法检出4株,组合纸片法检出4株,PCR法检测出4株(6.45%,4/62)金属酶阳性菌株,经全基因测序结果为IMP-1型,且全部为亚胺培南耐药菌株10%(4/40); 未检测出VIM,SPM和GIM型金属酶; PCR检测整合酶Ⅰ在亚胺培南耐药菌株中阳性率为40%(16/40),在亚胺培南敏感菌株中检出率为22.73%(5/22); 所有菌株均未检出整合酶Ⅱ,Ⅲ。结论 哈尔滨医科大学附属第二医院存在IMP-1型金属酶阳性的Pa,这些菌株多为多重耐药细菌,且携带Ⅰ类整合子,该院应做好防范工作,防止金属酶和整合子的传播。
Abstract:
Objective To investigate the situation of Pseudomonas aeruginosa strains carrying metallo-β-lactamases and integrases inthe Second Affiliated Hospital of Harbin Medical University.MethodsThe phenotype of metallo-β-lactamases were detected by modifiedHodge test,double-disc synergy and combination paper method,respectvily.PCRmethod was used to detecte metallo-β-lactamases genotypes and the integrationⅠ,Ⅱ and Ⅲ.The PCR products of the whole length bla-IMP gene were purified,sequenced and analyzed by Blast.Results Among 62 Pseudomonas aeruginosa strains,metallo-β-lactamases phenotype of 3 by modified hodge test,4 by double-disc synergy test,4 by combination paper method and were all positive 4 of IMP-1 type metallo-β-lactamases of Pseudomonasaeruginosa strains(10%,4/40)were positives by PCR.Integrase Ⅰ of 16(40%,16/40)strainswere positives by PCR method in IMP-resistant Pseudomonas aeruginosa,andintegrase Ⅰ of 22.73%(5/22)were positives in IMP-sensitive Pseudomonas aeruginosa.No other metallo-β-lactamases,integrase Ⅱ and Ⅲ were detectedin this study.Conclusion IMP-type metallo-β-lactamase existed in IMP-resistant Pseudomonas aeruginosa isolates of the Second Affiliated Hospital of Harbin Medical University.Most strains carried integraseⅠ,and other resistance mechanisms may be associated with multi-drug resistance,so it is important to prevent the Pseudomonas aeruginosa strains which carried metallo-β-lactamases and integrons widely spread in the hospital.

参考文献/References:

[1] Clinical and Laboratory Standards Institute.Performance Standards for Antimicrobial Susceptibility Testing Twentieth Informational Supplement[S].Wayne:PA,CLSIM100-S20,2010.
[2] Gome Franco MR,Caicaffa-Fiho HH,Burttini MN,et al.Metallo-beta-lactamases among imipenem-resistant Pseudomonas aeruginosa in a brazilian university hospital[J].Clinics,2010,65(9):825-829.
[3] Lee K,Chong Y,Shin HB,et al.Modified hodge and EDTA-disk synergy tests to screen metallo-β-lactamase producing strains of Pseudomonas and Acinetobacter species[J].Clin Microbiol Infect,2001,7(2):88-91.
[4] Lee K,Lim YS,Yong D,et al.Evaluation of the hodge test and the imipenem-EDTA double-disk synergy test for differentiating metallo-β-lactamase-producing isolates of Pseudomonas spp.and Acinetobacter spp[J].Clin Microbiol,2003,41(10):4623-4629.
[5] Qu TT,Zhang JL,Wang J,et al.Evaluation of phenotypic tests for detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa strains inChina[J].J Clin Microbiol,2009,47(4):1136-1142.
[6] Shibata N,Doi Y,Yamane K,et al.PCR typing of genetic determinants formetallo-β-laetamases and integrases carried by gram-negative bacteria isolated in Japan,with focus on the class 3 integron[J].J Clin Microbiol,2003,41(12):5407-5413.
[7] 汤凤珍,陈惠玲,张伟红.耐碳青霉烯类铜绿假单胞菌相关耐药基因及Ⅰ类整合子的研究[J].现代医院,2010,10(3):26-28. Tang FZ,Chen HL,Zhang WH,et al.Research on drug-resistant genesand the class Ⅰ integron of carbapenem resistant Pseudomonas aeruginosa[J].Modem Hospital,2010,10(3):26-28.
[8] 崔 巍,陈 雷.耐碳青霉烯类铜绿假单胞菌产金属β-内酰胺酶的检测及耐药性分析[J].中华感染学杂志,2011,21(3):560-562. Cui W,Chen L.Detection of carbapenems-resistant Pseudomonas aeruginosa producing metallo-β-lactamase and antibiotics resistance[J].Chinese Journal of Nosocomiology,2011,21(3):560-562.
[9] 吴爱武,李红玉.院内感染铜绿假单胞菌金属酶的检测及分析[J].广州医学院学报,2005,33(3):14-16. Wu AW,Li HY.Metallo-β-lactamase detection and antimicrobial resistance analysis of Pseudomonas aeruginosa causing nosocomial infection[J].Academic Journal of Guangzhou Medical College,2005,33(3):14-16.
[10] 吴长生,伍严安,余菲菲,等.铜绿假单胞菌整合子及其基因盒与耐药性的相关性研究[J].福建医科大学学报,2011,45(5):355-359. Wu CS,Wu YA,Yu FF,et al.Study on relationship between Pseudomonas aeruginosa integrons and antibiotic-resistance[J].J Fujian Med Univ,2011,45(5):355-359.
[11] 郭 宇,孙媛媛,张 正.铜绿假单胞菌整合子基因盒的相关研究[J].中国感染与化疗杂志,2011,11(1):27-30. Gao Y,Sun YY,Zhang Z.Study on the integrons and integrated gene cassettes in Pseudomonas aeruginosa[J].Chin J Infect Chemother,2011,11(1):27-30.
[12] 杜 艳,单 斌,陈瑞春,等.亚胺培南耐药的铜绿假单胞菌与Ⅰ类、Ⅲ类整合子的相关研究[J].现代检验医学杂志,2011,26(4):75-77. Du Y,Shan B,Chen RC,et al.Relative research between imipenem-resistant Pseudomonas aeruginosa and class Ⅰ and Ⅱ integrons[J].Journal of Modern Laboratory Medicine,2011,26(4):75-77.
[13] 多丽波,张联博,栾 英,等.哈尔滨地区铜绿假单胞菌外膜蛋白D2与亚胺培南耐药关系研究[J].临床检验杂志,2010,28(2):147-148. Duo LB,Zhang LB,Luan Y,et al.Pseudomonas ae-ruginosa in Harbin outer membrane protein D2 and imide south study on the relationship between the resistance[J].Chin J Clin Lab Sci,2010,28(2):147-148.

相似文献/References:

[1]邱晓明,林锦骠,黄 尔,等.黏液型与非黏液型铜绿假单胞菌Cif基因表达研究[J].现代检验医学杂志,2016,31(03):19.[doi:10.3969/j.issn.1671-7414.2016.03.006]
　QIU Xiao-ming,LIN Jin-piao,HUANG Er,et al.Investigation of Cif Gene Expression between Mucoid and Non-mucoid Pseudonmonas Aeruginosa[J].Journal of Modern Laboratory Medicine,2016,31(01):19.[doi:10.3969/j.issn.1671-7414.2016.03.006]
[2]陈乔彬,刘爱胜,文 艳.深圳地区三级区属医院铜绿假单胞菌感染分布及耐药性分析[J].现代检验医学杂志,2015,30(03):147.[doi:10.3969/j.issn.1671-7414.2015.03.045]
　CHEN Qiao-bin,LIU Ai-sheng,WEN Yan.Infection Distribution and Drug Resistance Analysis of Pseudomonas Aeruginosa in Third District Hospital in Shenzhen Area[J].Journal of Modern Laboratory Medicine,2015,30(01):147.[doi:10.3969/j.issn.1671-7414.2015.03.045]
[3]罗史科,刘鲜花,朱平安,等.铜绿假单胞菌对耐喹诺酮药物质粒基因的研究[J].现代检验医学杂志,2015,30(01):39.[doi:10.3969/j.issn.1671-7414.2015.01.011]
　LUO Shi-ke,LIU Xian-hua,ZHU Ping-an,et al.Research on Pseudomonas Aeruginosa Quinolone-resistant Plasmid Genes[J].Journal of Modern Laboratory Medicine,2015,30(01):39.[doi:10.3969/j.issn.1671-7414.2015.01.011]
[4]谢国艳,高志生,许 俊,等.Cica-Beta Test试剂盒检测 耐亚胺培南铜绿假单胞菌金属β-内酰胺酶的评估[J].现代检验医学杂志,2015,30(01):123.[doi:10.3969/j.issn.1671-7414.2015.01.036]
　XIE Guo-yan,GAO Zhi-sheng,XU Jun,et al.Evaluation of Cica-Beta Test Kit for Detection of Metallo-β-Lactamase-Producing Pseudomonas Aeruginosa[J].Journal of Modern Laboratory Medicine,2015,30(01):123.[doi:10.3969/j.issn.1671-7414.2015.01.036]
[5]李瑜珍,曾学辉,莫 莉,等.VITEK2 Compact全自动微生物分析仪对黏液型和非黏液型铜绿假单胞菌药敏检测评价[J].现代检验医学杂志,2016,31(04):121.[doi:10.3969/j.issn.1671-7414.2016.04.034]
　LI Yu-zhen,ZENG Xue-hui,Mo-li,et al.Evaluation of Drug Susceptibility Test to Mucoid Pseudomonas Aeruginosa and Non-Mucoid Pseudomonas Aeruginosa with VITEK2 Compact Automatic Microbiology Analyzer[J].Journal of Modern Laboratory Medicine,2016,31(01):121.[doi:10.3969/j.issn.1671-7414.2016.04.034]
[6]王 静,陈 葳,曾晓艳,等.不同标本来源铜绿假单胞菌对β-内酰胺酶耐药表型的差异性分析[J].现代检验医学杂志,2017,32(03):63.[doi:10.3969/j.issn.1671-7414.2017.03.017]
　WANG Jing,CHEN Wei,ZENG Xiao-yan,et al.Analysis of the Difference of β-Lactamase-Resistant Phenotypes by Pseudomonas Aeruginosa from Different Specimens[J].Journal of Modern Laboratory Medicine,2017,32(01):63.[doi:10.3969/j.issn.1671-7414.2017.03.017]
[7]谢国艳,肖 敏.铜绿假单胞菌D-试验阳性及阴性菌株产β-内酰胺酶的差异性分析[J].现代检验医学杂志,2018,33(03):130.[doi:10.3969/j.issn.1671-7414.2018.03.033]
　XIE Guo-yan,XIAO Min.Study on the Difference of β-Lactamases-Producing in D-Test Positive and D-Test Negative Pseudomonas Aeruginosa[J].Journal of Modern Laboratory Medicine,2018,33(01):130.[doi:10.3969/j.issn.1671-7414.2018.03.033]
[8]武爱荣,杨　乐.VITEK-2 Compact 检测奇异变形杆菌、摩根摩根菌、铜绿假单胞菌的部分药敏结果准确性评价[J].现代检验医学杂志,2020,35(06):106.[doi:doi:10.3969/j.issn.1671-7414.2020.06.026]
　WU Ai-rong,YANG Le.Accuracy Evaluation of Partial Drug Sensitivity Results of Vitek-2 CompactDetection for Proteus Singularis, Morgan Morgan and Pseudomonas Aeruginosa[J].Journal of Modern Laboratory Medicine,2020,35(01):106.[doi:doi:10.3969/j.issn.1671-7414.2020.06.026]
[9]黄秋兰,钱巧慧,范德平,等.鱼腥草联合亚胺培南对碳青霉烯耐药铜绿假单胞菌杀菌效果的体外研究[J].现代检验医学杂志,2021,36(03):118.[doi:10.3969/j.issn.1671-7414.2021.03.027]
　HUANG Qiu-lana,QIAN Qiao-hui,FAN De-pinga,et al.Vitro Study on the Bactericidal Effect of Houttuynia Cordata Combined withImipenem Against Carbapenem Resistant Pseudomonas Aeruginosa[J].Journal of Modern Laboratory Medicine,2021,36(01):118.[doi:10.3969/j.issn.1671-7414.2021.03.027]
[10]尚佳文,徐文娜,许南松,等.mCIM 和碳青霉烯酶抑制剂增强试验检测CRE 和CRPA 产酶表型的方法学评价[J].现代检验医学杂志,2023,38(03):165.[doi:10.3969/j.issn.1671-7414.2023.03.030]
　SHANG Jia-wen,XU Wen-na,XU Nan-song,et al.Methodological Evaluation of mCIM and Carbapenemase Inhibitor-enhancing Assays to Detect CRE and CRPA Enzyme Producing Phenotypes[J].Journal of Modern Laboratory Medicine,2023,38(01):165.[doi:10.3969/j.issn.1671-7414.2023.03.030]

备注/Memo

备注/Memo:
基金项目:黑龙江省教育厅科学技术研究项目(12531830)。
作者简介:多丽波(1966-),女,博士,硕导,主任医师,研究方向:细菌耐药性研究及耐药机制研究,Tel:13654678383,E-mail:duolibo@163.com。
更新日期/Last Update: 2015-01-30