[1]罗史科,刘鲜花,朱平安,等.铜绿假单胞菌对耐喹诺酮药物质粒基因的研究[J].现代检验医学杂志,2015,30(01):39-41.[doi:10.3969/j.issn.1671-7414.2015.01.011]
 LUO Shi-ke,LIU Xian-hua,ZHU Ping-an,et al.Research on Pseudomonas Aeruginosa Quinolone-resistant Plasmid Genes[J].Journal of Modern Laboratory Medicine,2015,30(01):39-41.[doi:10.3969/j.issn.1671-7414.2015.01.011]
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铜绿假单胞菌对耐喹诺酮药物质粒基因的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年01期
页码:
39-41
栏目:
论著
出版日期:
2015-01-30

文章信息/Info

Title:
Research on Pseudomonas Aeruginosa Quinolone-resistant Plasmid Genes
文章编号:
1671-7414(2015)01-039-04
作者:
罗史科刘鲜花朱平安樊春卉
深圳市盐田区人民医院检验科,广东深圳 518081
Author(s):
LUO Shi-keLIU Xian-huaZHU Ping-anFAN Chun-hui
Departement of Clinic Laboratory, Shenzhen Yantian Area People's Hospital,Guangdong Shenzhen 518081,China
关键词:
铜绿假单胞菌 喹诺酮 质粒 基因
分类号:
R378.991; Q786
DOI:
10.3969/j.issn.1671-7414.2015.01.011
文献标志码:
A
摘要:
目的 探讨铜绿假单胞菌对喹诺酮类药物的耐药质粒与周围共生菌耐药质粒染色体相互转移的关系。方法 收集深圳市第七人民医院2011年1月~2013年12月481份各种样品培养出的铜绿假单胞菌31例,药敏实验结果对喹诺酮耐药的15例为研究对象,运用质粒转化、提取实验证实其耐药质粒的存在,PCR扩增、基因序列分析,与周围共生菌耐药质粒的基因序列比对。结果 铜绿假单胞菌耐药株的耐药质粒与周围共生菌质粒发现有相同基因序列(χ2检验2=1.207,P<0.01)。结论 耐药质粒能在不同种属细菌之间传递。
Abstract:
Objective Pseudomonas aeruginosa resistanceto quinolones and around symbiotic bacteria resistant plasmids each chromosomemetastasis.Methods 481 samples was collected from the Seventh People's Hospital of Shenzhen since January 2011 to December 2013,and itcultured Pseudomonas aeruginosa 31 cases,susceptibility testing confirmed15 cases of quinolone-resistant as the research object,using plasmid transformation,pick up experiments confirmed the presence of the resistance plasmid,PCRamplification,gene sequence analysis,and gene sequences surrounding the symbiotic bacteria resistant plasmids as a same.Results The same gene sequence of plasmid was found between drug-resistant strains of Pseudomonas aeruginosa and the surrounding symbiotic bacteria[χ2=1.207,P<0.01].Conclusion Resistance plasmids could be transferred between different species of bacteria.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:深圳市盐田区产业发展资金,项目编号:2012[深盐产纪]5号。
作者简介:罗史科(1967-),男,本科,副主任技师,从事分子生物学工作,研究方向:耐药菌基因,Tel:0755-25215354,E-mail:luoshike@163.com。
更新日期/Last Update: 2015-01-30