[1]王小丽,刘瑞东.尿TGF-β1检测在诊断慢性肾炎中的临床应用[J].现代检验医学杂志,2015,30(01):88-90.[doi:10.3969/j.issn.1671-7414.2015.01.024]
 WANG Xiao-li,LIU Rui-dong.Clinical Application of Urinary Transforming Growth Factor-Beta(TGF-β1)Measurement in Diagnosing Chronic Nephritis[J].Journal of Modern Laboratory Medicine,2015,30(01):88-90.[doi:10.3969/j.issn.1671-7414.2015.01.024]
点击复制

尿TGF-β1检测在诊断慢性肾炎中的临床应用()
分享到:

《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年01期
页码:
88-90
栏目:
论著
出版日期:
2015-01-30

文章信息/Info

Title:
Clinical Application of Urinary Transforming Growth Factor-Beta(TGF-β1)Measurement in Diagnosing Chronic Nephritis
文章编号:
1671-7414(2015)01-088-03
作者:
王小丽刘瑞东
泰山医学院附属莱芜市人民医院检验科,山东莱芜 271100
Author(s):
WANG Xiao-liLIU Rui-dong
Department of Clinical Laboratory,Laiwu Municipal People's Hospital Affiliated Taishan Medical College,Shandong Laiwu 271100,China
关键词:
转化生长因子-β1 系膜增生性肾炎 局灶性肾小球硬化症 IgA 肾炎
分类号:
R692.3; R392.11
DOI:
10.3969/j.issn.1671-7414.2015.01.024
文献标志码:
A
摘要:
目的 研究慢性肾炎患者尿转化生长因子β1(TGF-β1)水平的变化及临床意义。方法 以22例健康者作为正常对照组(Norm),与40例慢性肾脏疾病患者尿TGF-β1水平作比较,利用逆转录聚合酶链反应(RT-PCR)和酶联免疫吸附试验(ELISA)的方法检测尿TGF-β1水平。结果 与正常对照组(31.53±11.59 ng/mmol.Cr)相比较,16例系膜增生性肾炎(MPG)患者(76.44±24.29 ng/mmol.Cr),14例局灶性肾小球硬化症(FGS)患者(65.07±20.59 ng/mmol.Cr)和10例IgA肾炎(IgA N)患者的尿TGF-β1水平(67.01±21.95 ng/mmol.Cr)明显增高,且差异均有统计学意义(P<0.01)。结论 尿TGF-β1水平变化可作为反映慢性肾炎的病情、判断病理类型及疾病诊断和疗效观察的重要指标。
Abstract:
Objective To investigate the level of urinary TGF-β1 and evaluate its clinical significance in patients with chronic nephritis.Methods 22 healthy people and 40 patients with chronicnephritis were selected,the levels of urinary TGF-β1 were measured by RT-PCR and ELISA.Results Urinary TGF-β1 were significantlyincreased in Mesangial Proliferative Glomerulonephritis(76.44±24.29 ng/mmol.Cr vs 31.53±11.59 ng/mmol.Cr,P<0.01); Focal Glomerular Sclerosis(65.07±20.59 ng/mmol.Cr vs 31.53±11.59 ng/mmol.Cr,P<0.01)and IgA Nephritis(67.01±21.95 ng/mmol.Cr vs 31.53±11.59 ng/mmol.Cr,P<0.01),comparing with normal group.Conclusion The levels ofurinary TGF-β1 could be a predictor in reflecting the sate of renal diease,estimating pathological type,diagnosing the renal diease and observing the therapeutic effects.

参考文献/References:

[1] 美国NKF-K/DoQI工作组.慢性肾脏病及透析的临床实践指南[M].王海燕,王 梅,译.北京:人民卫生出版社, 2003:13. The United States NKF-K/DoQI Working Group.Cl-inical practice guidelines of chronic kidney disease and dialysis[M].Wang HY,Wang M,Translated.Beijing:People's Medical Publishing House,2003:13.
[2] do Nascimento JF, Canani LH, Gerchman F,et al.Messenger RNA levels ofpodocyte-associated proteins in subjects with different degrees of glucose tolerance with or without nephropathy[J].BMC Nephrol,2013,14(1):214.
[3] Border WA,Noble NA.Transforming growth factor betain tissue fibrosis[J].N Engl J Med,1994,331(19):1286-1292.
[4] Liu YH.Epithelial to mesenchymal transition in renal fibrogenesis:pathologic significance,molecular mechanism,and therapeutic intervention[J].J AmSoc Nephrol,2004,15(1):1-12.
[5] Kasinath BS.Glomerular endothelial cell proteoglycans-regulation by TGF-beta 1[J].Arch Biochem Biophys,1993,305(2):370-377.
[6] Nakamura T,Miller D,Ruoslahti E,et al.Production of extracellular matrix by glomerular epithelial cells is regulated by transforming growth factor-beta 1[J].Kidney Int,1992,41(5):1213-1221.
[7] Remuzzi G,Ruggenenti P,Perico N.Chronic renal di-seases:renoprotectivebenefits of renin-angiotensin system inhibition[J].Ann Intern Med,2002,136(8):604-615.
[8] Edwards DR,Murphy G,Reynolds JJ,et al.Transforming growth factor betamodulates the expression of collagenase and metalloproteinase inhibitor[J].EMBO J,1987,6(7):1899-1904.
[9] Kaneto H,Morrissey J,Klahr S.Increased expression of TGF-beta 1 mRNAin the obstructed kidney of rats with unilateral ureteral ligation[J].KidneyInt,1993,44(2):313-321.
[10] Schneider A,Thaiss F,Rau HP,et al.Prostaglandin E1 inhibitscollagen expression in anti-thymocyte antibody-induced glomerulonephritis:Possible role of TGF beta[J].Kidney Int,1996,50(1):190-199.
[11] Zheng RP,Bai T,Zhou XG,et al.Lefty a protein inhibits TGF-β1-mediated apoptosis in human renal tubular epithelial cells[J].Mol Med Rep,2013,8(2):621-625.
[12] Kalliakmani P,Nakopoulou L,Tsakas S,et al.Urinary interleukin-6(IL-6)and transforming growth factor(TGF-β)levels in corticosteroidtreated patients with IgA nephropathy[J].Clin Nephrol,2011,76(2):144-150.
[13] Yamamoto T,Noble NA,Miller DE,et al.Sustained expression of TGF-β1underlies development of progressive kidney fibrosis[J].Kidney Int,1994,45(3):916-927.
[14] Agarwal R,Siva S,Dunn SR,et al.Add-on angi-otensin II receptor blockade lowers urinary transforming growth factor-beta levels[J].Am J Kidney Dis,2002,39(3):486-492.

相似文献/References:

[1]唐玉蓉a,王际亮a,孙婷婷a,等.儿童特发性血小板减少性紫癜Th亚群细胞因子的测定及意义[J].现代检验医学杂志,2015,30(05):143.[doi:10.3969/j.issn.1671-7414.2015.05.045]
 TANG Yu-ronga,WANG Ji-lianga,SUN Ting-tinga,et al.Determination and Clinical Significance of Th Subsets Cytokines in ITP of Children[J].Journal of Modern Laboratory Medicine,2015,30(01):143.[doi:10.3969/j.issn.1671-7414.2015.05.045]
[2]齐冰,孙蔚.肺间质纤维化并发肺气肿病人血清TGF-β,VEGF和IGF-I作用、意义及与吸烟相关性研究[J].现代检验医学杂志,2015,30(06):109.[doi:10.3969/j.issn.1671-7414.2015.06.033]
 QI Bing,SUN Wei.Research on the Significance and Role of the Serum TGF-β,VEGF, IGF-Ⅰand Its Correlation with Smoking of the Patients with Combined Pulmonary Fibrosis and Emphysema[J].Journal of Modern Laboratory Medicine,2015,30(01):109.[doi:10.3969/j.issn.1671-7414.2015.06.033]
[3]常 欣,代志彬,李贵贤,等.IGF-1,TGF-β1水平变化与过敏性紫癜患儿肾损害的关系[J].现代检验医学杂志,2017,32(03):101.[doi:10.3969/j.issn.1671-7414.2017.03.027]
 CHANG Xin,DAI Zhi-bin,LI Gui-xian,et al.Relationship between the Change of IGF 1 and TGF-β1 Level and Renal Damage in Children with Allergic Purpura[J].Journal of Modern Laboratory Medicine,2017,32(01):101.[doi:10.3969/j.issn.1671-7414.2017.03.027]

备注/Memo

备注/Memo:

作者简介:王小丽(1982-),女,学士,初级检验师,专业:临床检验,E-mail:twx111@163.com。
更新日期/Last Update: 2015-01-30