[1]王 欣,任超杰,扈会整,等.黏质沙雷菌的耐药率分析及碳青霉烯酶的检测[J].现代检验医学杂志,2015,30(01):118-120.[doi:10.3969/j.issn.1671-7414.2015.01.034]
　WANG Xin,REN Chao-jie,HU Hui-zheng,et al.Detection of Drug Resistance of Serratia Marcescens Rate Analysis and Carbapenemase[J].Journal of Modern Laboratory Medicine,2015,30(01):118-120.[doi:10.3969/j.issn.1671-7414.2015.01.034]
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黏质沙雷菌的耐药率分析及碳青霉烯酶的检测()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年01期
页码:
118-120
栏目:
研究简报·实验技术
出版日期:
2015-01-30

文章信息/Info

Title:
Detection of Drug Resistance of Serratia Marcescens Rate Analysis and Carbapenemase
文章编号:
1671-7414(2015)01-118-03
作者:
王 欣1; 2; 任超杰1; 2; 扈会整1; 郝晓柯2
1.陕西省核工业215医院检验科,陕西咸阳 712000;
2.第四军医大学西京医院检验科,西安 710032
Author(s):
WANG Xin1; 2; REN Chao-jie1; 2; HU Hui-zheng1; HAO Xiao-ke2
1.Department of Laboratory Medicine,Nuclear Industry,the 215Hospital of Shaanxi Province,Shaanxi Xianyang 712000,China;
2.Department of Clinical Laboratory, Xijing Hospital of the Fourth Military Medical University,Xi'an 710032,China
关键词:
黏质沙雷菌;  耐药率;  碳青霉烯酶
分类号:
R378.2; R446.5
DOI:
10.3969/j.issn.1671-7414.2015.01.034
文献标志码:
A
摘要:
目的 分析临床分离黏质沙雷菌对常规药物的耐药及产碳青霉烯酶的情况,指导临床合理用药。方法 收集陕西省核工业215医院2009～2012年临床分离的106株黏质沙雷菌,K-B纸片法进行药敏试验,改良Hodge试验筛选碳青霉烯酶,Whonet软件统计分析。结果 黏质沙雷菌在痰、分泌物、咽试子、尿液标本分别分离出89株(84.1%)、4株(3.8%)、3株(2.8%)和3株(2.8%)。黏质沙雷菌对氨苄西林和头孢唑林的耐药率超过90%,对氨曲南、头孢吡肟、头孢他啶、哌拉西林/他唑巴坦、头孢哌酮/舒巴坦的耐药率为10%～22%,对丁胺卡那霉素、环丙沙星和亚胺培南的耐药率均低于10%; 对亚胺培南耐药的菌株有4株,改良Hodge试验阳性菌3株,阳性率为2.8%。结论 黏质沙雷菌广泛耐药,其耐药机制十分复杂,该院已出现产碳青霉烯酶菌株,临床医师应根据药敏试验结果合理用药。
Abstract:
Objective To analyze the drug resistance in clinical isolates of Serratia marcescens of conventional treatment and carbapenemases,guiding the clinical rational drug use.Methods 106 strains of Serratia marcescens from 2009～2012 year the clinical isolation,K-B disk diffusion susceptibility test,the modified Hodge method for screening the carbapenemase,Whonet statistical analysis.Results Serratia marcescens in sputum,secretions,swallow swab,urine samples were isolated 89 strains(84.1%),4 strains(3.8%),3 strains(2.8%)and 3 strains(2.8%).Serratia marcescens resistant to ampicillin and cefazolin ratemore than 90%,to aztreonam,cefepime,ceftazidime,piperacillin/tazobactam andCefoperazone/sulbactam,drug resistance rate was 10%～22%,to amikacin,ciprofloxacin and imipenem were lower than 10%.Imipenem the resistant strains in 4 strains,the modified Hodge test positive 3 strains,the positive rate was 2.8%.Conclusion Serratia marcescens extensively drug-resistant strains,the resistant mechanism is very complex,the institute has appearedcarbapenemase producing strains,clinicians should be based on the drug sensitivity test results.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:陕西省科学技术研究发展计划项目(项目编号:2014K11-02-03-09)。
作者简介:王 欣(1980-),女,在读硕士,副主任检验师,从事临床医学检验工作,Tel:18691060643,E-mail:4136439@qq.com。
通讯作者:郝晓柯,男,教授,博士生导师,研究方向:分子生物学与实验室管理。
更新日期/Last Update: 2015-01-30