[1]谢国艳,高志生,许 俊,等.Cica-Beta Test试剂盒检测 耐亚胺培南铜绿假单胞菌金属β-内酰胺酶的评估[J].现代检验医学杂志,2015,30(01):123-125.[doi:10.3969/j.issn.1671-7414.2015.01.036]
 XIE Guo-yan,GAO Zhi-sheng,XU Jun,et al.Evaluation of Cica-Beta Test Kit for Detection of Metallo-β-Lactamase-Producing Pseudomonas Aeruginosa[J].Journal of Modern Laboratory Medicine,2015,30(01):123-125.[doi:10.3969/j.issn.1671-7414.2015.01.036]
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Cica-Beta Test试剂盒检测 耐亚胺培南铜绿假单胞菌金属β-内酰胺酶的评估()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年01期
页码:
123-125
栏目:
研究简报·实验技术
出版日期:
2015-01-30

文章信息/Info

Title:
Evaluation of Cica-Beta Test Kit for Detection of Metallo-β-Lactamase-Producing Pseudomonas Aeruginosa
文章编号:
1671-7414(2015)01-123-03
作者:
谢国艳高志生许 俊秦 云钱敏健
上海交通大学医学院附属新华医院崇明分院,上海 202150
Author(s):
XIE Guo-yanGAO Zhi-shengXU JunQIN YunQIAN Min-jian
Chongming Branch of Xinhua Hospital,Shanghai Jiaotong University,Shanghai 202150,China
关键词:
铜绿假单胞菌 金属β-内酰胺酶 HMRZ-8
分类号:
R378.991; R446
DOI:
10.3969/j.issn.1671-7414.2015.01.036
文献标志码:
A
摘要:
目的 评估Cica-Beta Test试剂盒对铜绿假单胞菌(PAE)金属β-内酰胺酶(MBLs)检测的临床适用性。方法 选取文献
[5]中的82株耐亚胺培南PAE,PAE-MHT法和Cica-Beta Test试剂盒分别进行MBLs检测。结果 PAE-MHT法的敏感度、特异度和准确率分别为84.6%,97.2%和97.6%。Cica-Beta Test试剂盒敏感度、特异度和准确率分别为76.9%,100%和96.3%。PAE-MHT法和Cica-Beta Test试剂盒有非常好的一致性。结论 两种方法均适合应用于临床快速筛查非发酵菌产MBLs。
Abstract:
Objective To evaluate Cica-Beta Test kit for detection of metallo-β-lactamase-producing Pseudomonas aeruginosa(PAE)inthe clinical microbiology laboratory.Methods A total of82 imipenem-resistant PAE clinical isolates from literature
[5]was dectectedto metallo-β-lactamase(MBLs)by PAE-MHT and Cica-Beta Test kit.Results The sensitivity,specificity and accuracy rate of PAE-MHTwas 84.6%,97.2% and 97.6%,and the sensitivity,specificity and accuracy rate of Cica-Beta Test kit was 76.9%,100% and 96.3%,respectively.Two methodshad a good consistency.Conclusion Two methods are simple,quick for detecting to metallo-β-lactamase-producing Pseudomonas in clinical laboratories.

参考文献/References:

[1] Colodner R,Reznik B,Gal V,et al.Evaluation of a novel kit forthe rapid detection of extended-spectrum beta-lactamases[J].Eur J Clin Microbiol Infect Dis,2006,25(1):49-51.
[2] 汪雅萍,杨 俊,应春妹,等.快速筛查革兰阴性杆菌β-内酰胺酶[J].中国感染与化疗杂志,2012,12(4):302-305. Wang YP,Yang J,Ying CM,et al.Quick screening test of beta-lactamase in gram-negative bacilli[J].Chin J Infer Chemother,2012,12(4):302-305.
[3] 吴 琼,张祎博,倪语星.细菌β-内酰胺酶快速检测及分型试剂盒的应用评估[J].检验医学,2010,25(1):36-37. Wu Q,Zhang WB,Ni YX.Evaluation of a novel kit for identifying and differentiating of beta-lactamase[J].Laboratory Medicine,2010,25(1):36-37.
[4] Pasteran F,Veliz O,Rapoport M,et al.Sensitive and specific modified Hodge test for KPC and metallo-beta-lactamase detection in Pseudomonas aeruginosa by use of a novel indicator strain,Klebsiella pneumoniae ATCC 700603[J].J Clin Microbiol,2011,49(12):4301-4303.
[5] 谢国艳,高志生,许 俊,等.PAE-MHT法检测耐亚胺培南铜绿假单胞菌金属β-内酰胺酶的临床适用性的评估[J].中国实验诊断学,2014,18(6):982-985. Xie GY,Gao ZS,Xu J,et al.Evaluation of Pseudomonas aeruginosa-modified hodge test for detection of metallo-β-lactamase-producing Pseudomonas aeruginosa[J].Chin J Lab Diagn,2014,18(6):982-985.
[6] Bush K,Jacoby GA,Mederios AA.A functional classification scheme for beta-lactamases and its correlation with molecular structure[J].Antimicrob Agents Chemother,1995,39(6):1211-1233.
[7] Hanaki H,Kubo R,Nakano T,et al.Characterization of HMRZ-86:a novelchromogenic cephalosporin for the detection of extended-spectrum beta-lactamases[J].J Antimicrob Chemother,2004,53(5):888-889.

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备注/Memo

备注/Memo:
基金项目:上海市崇明社会发展基金(ck201136)。
作者简介:谢国艳(1974-),女,硕士,副主任检验师,研究方向:主要从事微生物学检验与耐药基因分子诊断研究,E-mail:xieguoyan93@163.com。
通讯作者:高志生(1974-),男,硕士,副教授,副主任医师,E-mail:gaozhishen93@sina.com。
更新日期/Last Update: 2015-01-30