[1]孙灵迪,梅传忠,王平均,等.PCR-SBT与IMS-ELISA法在强直性脊柱炎患者HLA-B27检测中的比较[J].现代检验医学杂志,2015,30(02):16-18.[doi:10.3969/j.issn.1671-7414.2015.02.005]
　SUN Ling-di,MEI Chuan-zhong,WANG Ping-jun,et al.Comparison Analysis of HLA-B27 Detected by PCR-SBT and IMS-ELISA in AS Patients[J].Journal of Modern Laboratory Medicine,2015,30(02):16-18.[doi:10.3969/j.issn.1671-7414.2015.02.005]
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PCR-SBT与IMS-ELISA法在强直性脊柱炎患者HLA-B27检测中的比较()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年02期
页码:
16-18
栏目:
论著
出版日期:
2015-03-20

文章信息/Info

Title:
Comparison Analysis of HLA-B27 Detected by PCR-SBT and IMS-ELISA in AS Patients
作者:
孙灵迪1; 2; 梅传忠1; 王平均2; 武晓茜2; 邵先安1; 2
1.蚌埠医学院,安徽蚌埠 233030; 2.解放军第123医院检验病理科,安徽蚌埠 233015
Author(s):
SUN Ling-di1; 2; MEI Chuan-zhong1; WANG Ping-jun2; WU Xiao-qian2; SHAO Xian-an1; 2
1.Bengbu Medical College,Anhui Bengbu 233030,China; 2.Clinical Laboratory and Pathological Department,the 123 Hospital of PLA,Anhui Bengbu 233015,China
关键词:
聚合酶链反应-直接测序法;  磁珠酶联免疫法;  人类白细胞抗原B27;  强直性脊柱炎
分类号:
R593.23; Q503
DOI:
10.3969/j.issn.1671-7414.2015.02.005
文献标志码:
A
摘要:
目的 比较聚合酶链反应-直接测序法(polymerase chainreaction sequence-based typing,PCR-SBT)和磁珠酶联免疫法(immunomagnetic separation and enzyme-linked immunosorbent assay,IMS-ELISA)检测人类白细胞抗原B27(human leukocyte antigen B27,HLA-B27)在强直性脊柱炎(ankylosing spondylitis,AS)临床诊断中的价值。方法 应用PCR-SBT法和IMS-ELISA法对120例疑似AS患者外周血进行HLA-B27检测。以SPSS17.0软件的配对χ2检验和受试者工作特征曲线下面积对两种方法检测HLA-B27在AS诊断中的价值进行评价。结果 120例疑似AS患者中,PCR-SBT和IMS-ELISA法HLA-B27检测阳性率分别为45.83%(55/120)和37.50%(45/120)。两种方法检测HLA-B27差异有统计学意义(χ2=59.455,P=0.000)。PCR-SBT法敏感度和特异度分别为96.36%和96.92%; IMS-ELISA法的敏感度和特异度分别为69.09%和89.23%。两者的AUC分别为0.966和0.792。结论与IMS-ELISA法相比,在AS的临床诊断中PCR-SBT法检测HLA-B27的敏感度和特异度更高,方法更优。
Abstract:
Objective To compare PCR-SBT to IMS-ELISA inthe HLA-B27 detection in the ankylosing spondylitis(AS)patients.Methods Simultaneously,PCR-SBT and IMS-ELISA were used to detect the HLA-B27 expression in peripheral blood samples which were suspected patientswith AS from 120 cases.Chisquare test of paired design and the area under curve of receiver operating characteristics of SPSS17.0 software were used to evaluate the value of PCR-SBT and IMS-ELISA in HLA-B27 detection of AS patients.Results Among 120 cases of suspected patients with AS,the positive rates of HLA-B27 detected by PCR-SBT and IMS-ELISA were 45.83%( 55/120)and 37.50%(45/120),respectively.There was statistical difference between the two methods in the HLA-B27 detection(χ2=59.455,P=0.000).Thesensibility and specificity of PCR-SBT were 96.36% and 96.92%,respectively.While the sensibility and the specificity of IMS-ELISA were 69.09% and 89.23%,respectively.Area under the curve of two methods were 0.966 and 0.792,respectively.Conclusion In comparison with IMS-ELISA,the sensibility and the specificity of PCR-SBT in HLA-B27 detection were higher inAS diagnosis,that is to say, PCR-SBT is better in HLA-B27 detection and AS diagnosis.

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备注/Memo

备注/Memo:
基金项目:南京军区医学科技课题资助项目(12MB015)。 作者简介:孙灵迪(1986-),男,硕士研究生,研究方向:分子免疫诊断,Tel:18305523929。 通讯作者:邵先安,男,副教授,硕士生导师,研究方向:分子/基因免疫,E-mail:XAShao@126.com。
更新日期/Last Update: 2015-03-20