[1]邓安彦,蔡艳娟,周守容,等.ELISA法检测HBsAg(CMIA)低值血清样本的结果分析[J].现代检验医学杂志,2015,30(02):123-125.[doi:10.3969/j.issn.1671-7414.2015.02.038]
　DENG An-yan,CAI Yan-juan,ZHOU Shou-rong,et al.Analysis of the Results of ELISA by Detecting Low Quantitative HBsAg(CMIA)in Serums[J].Journal of Modern Laboratory Medicine,2015,30(02):123-125.[doi:10.3969/j.issn.1671-7414.2015.02.038]
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ELISA法检测HBsAg(CMIA)低值血清样本的结果分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年02期
页码:
123-125
栏目:
研究简报·实验技术
出版日期:
2015-03-20

文章信息/Info

Title:
Analysis of the Results of ELISA by Detecting Low Quantitative HBsAg(CMIA)in Serums
作者:
邓安彦1; 蔡艳娟2; 周守容1; 王 强2; 3; 王东升2; 张国元2; 凡瞿明2; 郭晓兰2; 3
1.南充市中心医院输血科,四川南充 637000; 2.川北医学院附属医院检验科,四川南充 637000; 3.川北医学院医学检验系,四川南充 637000
Author(s):
DENG An-yan1; CAI Yan-juan2; ZHOU Shou-rong1; WANG Qiang2; 3;  WANG Dong-sheng2; ZHANG Guo-yuan2; FAN Qu-ming2; GUO Xiao-lan2; 3
1.Department of Clinical Transfusion,Nanchong Central Hospital, Sichuan Nanchong 637000,China; 2.Department of Laboratory Medicine,Affiliated Hospital of North Sichuan Medical College,Sichuan Nanchong 637000,China; 3.Faculty of Laboratory Medicine,North Sichuan Medical College,Sichuan Nanchong 637000,China
关键词:
酶联免疫吸附试验;  化学发光法;  乙肝表面抗原;  低值
分类号:
R446.61; R512.62
DOI:
10.3969/j.issn.1671-7414.2015.02.038
文献标志码:
A
摘要:
目的 通过对HBsAg低值血清样本的检测,评价ELISA方法的检测性能。方法 收集化学发光法(CMIA)检测结果为0.05～9.99IU/ml的HBsAg低值血清样本305份,采用ELISA方法进行复孔检测。结果 HBsAg低值样本占HBsAg阳性样本的18.12%,主要分布于中老年人群。ELISA方法的总检出率为87.87%,其中0.05～0.11,0.12～0.20,0.21～0.50,0.51～1.00,1.01～5.00 IU/ml和5.01～9.99 IU/ml水平的检出率分别为36.00%,61.11%,78.38%,84.62%,99.11%和100.00%,各水平检出率之间差异有明显统计学意义(χ2=99.84,P=0.000)。ELISA方法检测HBsAg低值样本的S/CO结果与CMIA方法检测结果存在较高的相关性(r=0.874,P=0.000)。结论 ELISA方法检测HbsAg低值血清存在漏检的情况,临床实验室应根据实验目的合理选择实验方法。
Abstract:
Objective To evaluate the performance of ELISAby detecting low quantitative HBsAg in serums.Methods 305 serum samples that the quantitation range was from 0.05 IU/ml to 9.99 IU/mlwere collected,and then detected by ELISA.Results Therate of patients with low quantitation of HBsAg was 18.12% in patients with positive HBsAg.The total detected rate of ELISA was 87.87%,and the rate of 0.05～0.11,0.12～0.20,0.21～0.50,0.51～1.00,1.01～5.00 IU/ml and 5.01～9.99IU/ml were 36.00%,61.11%,78.38%,84.62%,99.11% and 100.00%,respectively.The differences were statistically significant between the detected rates ofeach group(χ2=99.84,P=0.000).There was high correlation coefficient between the results detected by ELISA and by CMIA(r=0.874,P=0.000).Conclusion The clinical laboratory should be careful to applythe method of ELISA to detect HBsAg for its missing detection in samples with low quantitation of HBsAg.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:邓安彦(1972-),男,学士学位,副主任技师,主要从事临床输血检验与相关检验、技术教学与管理工作,E-mail:331401181@qq.com。 通讯作者:郭晓兰,女,教授,主要从事临床免疫学检验与研究,E-mail:alan5200@hotmail.com。
更新日期/Last Update: 2015-03-20