[1]费阳,王 薇,王治国.临床实验室荧光原位杂交技术的质量管理[J].现代检验医学杂志,2015,30(02):149-151,155.[doi:10.3969/j.issn.1671-7414.2015.02.048]
　FEI Yang,WANG Wei,WANG Zhi-guo.Quality Management of Fluorescence in Situ Hybridization Methods for Clinical Laboratories[J].Journal of Modern Laboratory Medicine,2015,30(02):149-151,155.[doi:10.3969/j.issn.1671-7414.2015.02.048]
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临床实验室荧光原位杂交技术的质量管理()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年02期
页码:
149-151,155
栏目:
质量控制·实验室管理
出版日期:
2015-03-20

文章信息/Info

Title:
Quality Management of Fluorescence in Situ Hybridization Methods for Clinical Laboratories
作者:
费阳; 王 薇; 王治国
北京医院卫生部临床检验中心,北京 100730
Author(s):
FEI Yang; WANG Wei; WANG Zhi-guo
National Center for Clinical Laboratories,Beijing Hospital of the Ministry of Health,Beijing 100730,China
关键词:
细胞遗传学;  荧光原位杂交;  质量管理
分类号:
Q503
DOI:
10.3969/j.issn.1671-7414.2015.02.048
文献标志码:
A
摘要:
荧光原位杂交(fluorescent in situ hybridization,FISH)技术在很多临床实验室已经成为一项常规技术了。它可以用于检测对传统细胞遗传学分析来说太小而不能识别,但是对于标准脱氧核糖核酸(deoxyribonucleic acid,DNA)测序来说太大而不能检查的遗传畸变。如今,FISH不仅用于医学遗传学,也在很多其它领域包括神经科学、进化生物学、微生物学、病理学、毒理学和生殖遗传学内使用。FISH技术的迅速发展使得开展FISH的临床实验室对FISH的质量要求越来越高。该文对FISH的质量管理进行了具体描述,旨在提高实验室检测结果的准确度和可靠性,从而提高临床满意度并且改善病人预后。希望能够给开展FISH的临床实验室提供参考。
Abstract:
FISH technology has become a routine technique in many clinical laboratories.It can be used for the examination of genetic aberrations that are too small to visualize by conventional cytogenetic assays,buttoo large to detect by standard DNA sequencing.Today,FISH applications are used not only in medical genetics,but also in many other disciplines including neuroscience,evolutionary biology,microbiology,pathology,toxicology,and reproductive genetics.As FISH technology keep developing, the requirement of the results of FISH is increasingly high.This paper described the quality management for FISH detailedly,aimed at improving the accuracy and reliability of the result of FISH,satisfying the requirements of clinicians and patients and improvingthe healthcare quality and outcome of patients,hoping for providing some references for laboratories who develop FISH technology.

参考文献/References:

[1] Pinkel D,Straume T,Gray JW.Cytogenetic analysis using quantitative,high-sensitivity,fluorescence hybridization[J].Proc Natl Acad Sci USA,1986,83(9):2934-2938.
[2] Lichter P,Cremer T,Borden J,et al.Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridizationusing recombinant DNA libraries[J].Hum Genet,1988,80(3):224-234.
[3] Levsky JM,Singer RH.Fluorescence in situ hybridization:past,present and future[J].J Cell Sci,2003,116(Pt 14):2833-2838.
[4] International Organization for Standardization.Medical laboratories-particular requirements for quality and competence[S].Switzerland:Geneva ISO15189,2007.
[5] Berte LM.Laboratory quality management:a roadmap[J].Clin Lab Med,2007,27(4):771-790.
[6] CLSI QMS02-A6.Quality management system:development and management oflaboratory documents; Approved Guideline-Sixth Edition[S].Wayne,PA:CLISQMS02-A6,2013.
[7] Clinical and Laboratory Standards Institule QMS01-A4.Quality ManagementSystem:A model for laboratory services; Approved Guideline-Fourth Edition[S].Wayne:PA,CLSI QMS01-A4,2011.
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[1]明盛金a,黄 滢a,马洪熹a,等.1986～2022 年广西梧州地区遗传咨询者细胞遗传学检测结果及变化分析[J].现代检验医学杂志,2023,38(04):143.[doi:10.3969/j.issn.1671-7414.2023.04.026]
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备注/Memo

备注/Memo:
作者简介:费阳(1991-),女,在读硕士研究生,研究方向:实验室质量管理,E-mail:277475101@qq.com。 通讯作者:王治国,硕士,研究员,主要从事临床检验质量控制方法研究和室间质量评价工作,E-mail:zgwang@nccl.org.cn。
更新日期/Last Update: 2015-03-20