[1]何法霖,王 薇,钟 堃,等.血红蛋白A2和F的室间质量评价结果分析[J].现代检验医学杂志,2015,30(02):156-159.[doi:10.3969/j.issn.1671-7414.2015.02.050]
　HE Fa-lin,WANG Wei,ZHONG Kun,et al.External Quality Assessment of Hemoglobin A2(HbA2) and Hemoglobin F(HbF)Measurement[J].Journal of Modern Laboratory Medicine,2015,30(02):156-159.[doi:10.3969/j.issn.1671-7414.2015.02.050]
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血红蛋白A2和F的室间质量评价结果分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年02期
页码:
156-159
栏目:
质量控制·实验室管理
出版日期:
2015-03-20

文章信息/Info

Title:
External Quality Assessment of Hemoglobin A2(HbA2) and Hemoglobin F(HbF)Measurement
作者:
何法霖; 王 薇; 钟 堃; 王治国
卫生部北京医院 卫生部临床检验中心,北京 100730
Author(s):
HE Fa-lin; WANG Wei; ZHONG Kun; WANG Zhi-guo
Beijing Hospital of Ministry of Health,National Center for Clinical Laboratories,Beijing 100730,China
关键词:
室间质量评价;  血红蛋白A2;  血红蛋白F;  质量控制;  地中海贫血
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2015.02.050
文献标志码:
A
摘要:
目的 评价中国部分珠蛋白生成障碍性贫血筛查实验室检测血红蛋白A2和F的水平和现状。方法 向50家珠蛋白生成障碍性贫血实验室发放两个批号的质控品,收集实验室回报的HbA2和HbF检测值,对回报的结果按照方法分组进行统计分析,并评价其检测水平。结果 49家实验室回报了检测结果,回报率为98%。HbA2的及格率为42.9%～92.3%,HbF的及格率为27.3%～84.6%。通过稳健Z比分数统计,血红蛋白HbA2项目201311批号样本有3家实验室检测结果为不满意,201312批号样本有4家实验室检测结果为不满意,血红蛋白HbF项目201311批号样本有5家实验室检测结果为不满意,201312批号样本有3家实验室检测结果为不满意。结论 我国珠蛋白生成障碍性贫血筛查实验室检测HbA2和HbF质量有待进一步提高。
Abstract:
Objective To evaluate hemoglobin A2 and F(HbA2 and HbF)assays in part of Thalassemia screening laboratories in China.Methods Two lots of controls were sent to 50 Thalassemia screening laboratories.Test results of HbA2 and HbF were collected,analyzed and evaluated by different method groups.Results 49 of all laboratories reported the results and the rate of return was 98%.The proportion oflaboratories reporting acceptable results for HbA2 and HbF testing was 42.9～92.3% and 27.3%～84.6% respectively.HbA2 test results from 3 laboratories forLot 201311 and 4 for 201312 were unsatisfactory.And HbF test results from 5 laboratories for Lot 201311 and 3 for 201312 were unsatisfactory.Conclusion Conclude that quality in the measurement of HbA2 and HbF shouldbe improved.

参考文献/References:

[1] 熊 符,孙曼娜,娄季武,等.广西壮族自治区人群血红蛋白病的分子流行病学调查[C]. Xiong F,Sun MN,Lou JW,et al.Molecular epidemiological survey ofhaemoglobinopathies in Guangxi Zhuang Autonomous region[C].
[2] 郑 琳,黄海龙,范向群,等.高效液相色谱技术检测β-珠蛋白生成障碍性贫血的临床价值探讨[J],中国妇幼保健,2011,11:1165-1166. Zheng L,Huang HL,Fan XQ,et al.Exploration on the clinical valueof high performance liquid chromatography in detection of β-thalassemia[J].Maternal Child Health Care of China,2011,26(11):1165-1166.
[3] 张春荣.全自动蛋白电泳系统在筛查β珠蛋白生成障碍性贫血1913例中的应用[J].广西医学,2008,30(12):1866-1867. Zhang CR.Guangxi Medical Journal,2008,30(12):1866-1867.
[4] 卢业成,郑师陵,肖艳华,等.全自动多通道毛细管区带电泳技术在血红蛋白分析中的临床应用[J].国际检验医学杂志,2009,30(7):675-676,679. Lu YC,Zheng SL,Xiao YH,et al.Clinical application of fully automated multicapillary zone electrophoresis in hemoglobin analysis[J].Inter J Lab Med,2009,30(7):675-676,679.
[5] 陈 星,卢业成,初德强,等.全自动毛细管电泳与琼脂糖电泳检测血红蛋白的比较[J].广东医学,2009,30(5):778-780. Chen X,Lu YC,Chu DQ,et al.Automatic capillary electrophoresis with agarose electrophoresis detection of hemoglobin[J].Guangdong Medical Journal,2009,30(5):778-780.
[6] Keren DF,Hedstrom D,Gulbranson R,et al.Comparison of sebia capillaryscapillary electrophoresis with the primus high-pressure liquid chromatographyin the evaluation of hemoglobinopathies[J].Am J Clin Pathol,2008,130(5):824-831.

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备注/Memo

备注/Memo:
作者简介:何法霖(1981-),女,硕士,助理研究员,主要从事临床检验实验室质量管理与控制,Tel:010-58115056,E-mail:hefalin@126.com。
更新日期/Last Update: 2015-03-20