[1]唐海霞,王 成,路美玲,等.肾透明细胞癌患者血清miR-193a-3p水平增加及早期诊断价值[J].现代检验医学杂志,2015,30(03):43-45.[doi:10.3969/j.issn.1671-7414.2015.03.012]
　TANG Hai-xia,WANG Cheng,LU Mei-ling,et al.Elevated Serum miR-193a-3p Level in Renal Clear Cell Carcinoma and Its Early Clinical Value[J].Journal of Modern Laboratory Medicine,2015,30(03):43-45.[doi:10.3969/j.issn.1671-7414.2015.03.012]
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肾透明细胞癌患者血清miR-193a-3p水平增加及早期诊断价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年03期
页码:
43-45
栏目:
论著
出版日期:
2015-06-10

文章信息/Info

Title:
Elevated Serum miR-193a-3p Level in Renal Clear Cell Carcinoma and Its Early Clinical Value
文章编号:
1671-7414(2015)03-043-04
作者:
唐海霞; 王 成; 路美玲; 张春妮
南京大学医学院附属金陵医院检验科,南京 210002
Author(s):
TANG Hai-xia; WANG Cheng; LU Mei-ling; ZHANG Chun-ni
Department of Clinical Laboratory,Jingling Hospital of Medical College Nanjing University,Nanjing 210002,China
关键词:
肾透明细胞癌;  血清;  miR-193a-3p;  定量PCR;  早期诊断;  分子标志物
分类号:
R737.11; R730.43
DOI:
10.3969/j.issn.1671-7414.2015.03.012
文献标志码:
A
摘要:
目的 检测肾透明细胞癌患者血清中miR-193a-3p的表达水平,探讨其作为肾透明细胞癌分子诊断指标的可能性。方法 收集南京军区南京总医院2010～2014年期间107例住院肾透明细胞癌患者(TNM分期Ⅰ期76例、Ⅱ期16例、Ⅲ期2例、Ⅳ期8例和未知5例)未经治疗前血清样本,实时荧光定量PCR(qRT-PCR)方法检测miR-193a-3p含量,并以107例年龄、性别匹配的非癌健康人作对照。ROC曲线分析血清miR-193a-3p水平对肾透明细胞癌的早期诊断价值。结果 qRT-PCR检测证实,miR-193a-3p在总体患者血清中的含量显著高于对照组(3.294 ± 1.526 vs 1.944±0.600,P=0.000)。TNM Ⅰ期(3.411±1.676 vs 1.944±0.600,P=0.000)、Ⅱ期(2.926±0.927 vs 1.944±0.600,P=0.001)和Ⅳ期患者(2.926±0.894 vs 1.944±0.600,P= 0.000)血清中miR-193a-3p的表达水平均明显高于对照组。用于诊断肾透明细胞癌早期(Ⅰ期)的血清miR-193a-3p ROC曲线下面积(AUCROC)为0.820(95% CI,0.756～0.883),敏感度为80.3%,特异度为93.5%。结论 miR-193a-3p在肾透明细胞癌患者血清中的水平显著升高,并有较高的早期诊断准确性,有望作为该病早期诊断的血清学指标。
Abstract:
Objective To determine the levels of miR-193a-3p in serum from patients with renal clear cell carcinoma,and evaluate the potential of miR-193a-3p asa molecular biomarker of renal clear cell carcinoma.Methods Serum samples were taken from 107 untreated hospitalized patients with renal clear cell carcinoma(TNM Ⅰ grade 76 cases,Ⅱ grade 16 cases,Ⅲ grade 2 cases,Ⅳ grade 8 cases and unknown 5 cases)in NanjingGeneral Hospital from 2010 to 2014 year and 107 age-and gender-matched healthy individuals to determine the level of miR-193a-3p by quantitative reverse-transcription PCR(qRT-PCR).The early diagnostic value of miR-193a-3p for renal clear cell carcinoma was assessed by ROC curve.Results qRT-PCR confirmed that serum miR-193a-3p expression levels was significantly elevated in cancer than in normal controls(3.294±1.526 vs 1.944±0.600,P=0.000).TNM Ⅰ grade(3.411±1.676 vs 1.944±0.600,P=0.000),Ⅱ grade(2.926±0.927 vs 1.944±0.600,P=0.001),Ⅳ grade(2.926±0.894 vs 1.944±0.600,P=0.000)is significantly higher than that in control group.The area under the ROC curve(AUC)of miR-193a-3p was 0.820(95% CI,0.756～0.883),demonstratinga high sensitivity(80.3%)and specificity(93.5%)for the early diagnosis of renal clear cell carcinoma.ConclusionMiR-193a-3p content was significantly elevated in serum from renal clear cell carcinoma and has a high accuracy in diagnosing early cancer,whichholds potential as molecular biomarker for renal clear cell carcinoma.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81171661; 81472021); 南京军区医学科技创新重点课题(12Z28); 南京军区南京总医院院管课题(2012058)。
作者简介:唐海霞(1984-),女,本科,技师,主要从事临床检验工作,E-mail:tanghaixia smile@163.com。
通信作者:张春妮(1963-),女,博士,主任技师,博士生导师,主要研究方向:临床检验诊断学,E-mail: zchunni27@hotmail.com。
更新日期/Last Update: 2015-06-10