[1]吴瑜霞,吴 斌,潘秀华,等.新型B族链球菌胶体金免疫层析试纸条的临床应用评价[J].现代检验医学杂志,2015,30(04):53-55.[doi:10.3969/j.issn.1671-7414.2015.04.014]
 WU Yu-xia,WU Bin,PAN Xiu-hua,et al.Clinical Application of Novel Colloidal Gold Immunochromatographic Test Strip for Group B Streptococci[J].Journal of Modern Laboratory Medicine,2015,30(04):53-55.[doi:10.3969/j.issn.1671-7414.2015.04.014]
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新型B族链球菌胶体金免疫层析试纸条的临床应用评价()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年04期
页码:
53-55
栏目:
论著
出版日期:
2015-08-10

文章信息/Info

Title:
Clinical Application of Novel Colloidal Gold Immunochromatographic Test Strip for Group B Streptococci
文章编号:
1671-7414(2015)04-053-03
作者:
吴瑜霞1吴 斌1潘秀华2汤永平2徐邦牢1
1.广州市第一人民医院检验科,广州 510180;
2.广州市微米生物科技有限公司,广州 510663
Author(s):
WU Yu-xia1WU Bin1PAN Xiu-hua2TANG Yong-ping2XU Bang-lao1
1.Department of Clinical Laboratory,Guangzhou First People's Hospital,Guangzhou 510180, China;
2.Guangzhou Weimi Biotechnology Co.,Ltd,Guangzhou 510663,China
关键词:
B族链球菌 胶体金免疫层析 试纸 临床应用
分类号:
R378.12; Q503
DOI:
10.3969/j.issn.1671-7414.2015.04.014
文献标志码:
A
摘要:
目的 探讨新型B族链球菌(GBS)胶体金免疫层析试纸条的临床检测效果。方法 选取202份女性的阴道或宫颈棉拭子标本,采用新型检测试剂与对照试剂作对比,进行临床应用评价。结果 新型试剂最低检出浓度为105 CFU/ml,5~8 min内即可观察结果; 与对照试剂相比,考核试剂灵敏度更好,显色时间更短; 检测的202份临床标本中,与对照试剂相比,197例样本检测结果一致,其余5例样本检测结果不符,阳性符合率达97.5%,阴性符合率达97.54%,总符合率为97.52%,Kappa值0.948,一致性分析表明新型试剂与对照试剂具有很好的一致性。结论 该新型试剂能有效的辅助诊断B族链球菌感染,具有较高的临床应用价值。
Abstract:
Objective To explore the effect of a novel colloidal gold immunochromatographic test strip for detection of group B streptococci(GBS).Methods A total of 202 cases of swab of vagina or neck of uterus were collected,and they were detected by novel strip and control strip to evaluate their clinical applications.Results Sensitivity of novel strip was about 105 CFU/ml and the detection time was about 5 to 8 minutes,and it showed better sensitivity and shorter detectiontime compared with control strip.In the 202 cases of clinical samples,the detection results of 197 cases were in consistent with the control strip,however,the detection results of 5 cases were not in consistent.The positive coincidencerate and negative coincidence rate were 97.5% and 97.54% respectively,and the total coincidence rate and Kappa value were 97.52% and 0.948 respectively.The consistency test showed no significant difference between this strip and control strip.Conclusion This method was a effective technology for diagnosing of infection caused by GBS,and had high value in clinical application.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:广东省科技计划项目(2012B040304015),广州市科技攻关计划项目(2010U1-E00681)。
作者简介:吴瑜霞(1970-),女,大学本科,从事医学检验工作,E-mail:christine wu2003@163.com。
通讯作者:徐邦牢,男,主任技师,博士研究生导师,Tel:020-81048082,E-mail:banglaoxu@163.com。
更新日期/Last Update: 2015-08-10