[1]李大鹏,王永清,吕春雷,等.创伤性失血性休克大鼠血浆乳酸脱氢酶的动态测定及价值[J].现代检验医学杂志,2015,30(05):56-57.[doi:10.3969/j.issn.1671-7414.2015.05.017]
　LI Da-peng,WANG Yong-qing,L Chun-lei,et al.Dynamic Observation and Value of Plasma Level of Lactate Dehydrogenase in Rats with Traumatic Hemorrhagic Shock[J].Journal of Modern Laboratory Medicine,2015,30(05):56-57.[doi:10.3969/j.issn.1671-7414.2015.05.017]
点击复制

创伤性失血性休克大鼠血浆乳酸脱氢酶的动态测定及价值()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年05期
页码:
56-57
栏目:
论著
出版日期:
2015-12-10

文章信息/Info

Title:
Dynamic Observation and Value of Plasma Level of Lactate Dehydrogenase in Rats with Traumatic Hemorrhagic Shock
作者:
李大鹏; 王永清; 吕春雷; 张永军; 王凤娇; 郑文哲
解放军第88医院，山东泰安271000
Author(s):
LI Da-peng; WANG Yong-qing; L Chun-lei; ZHANG Yong-jun; WANG Feng-jiao; ZHENG Wen-zhe
the 88th Hospital of PLA，Shandong Taian 271000，China
关键词:
创伤性; 失血性休克; 动物模型; 组织细胞; 损伤; 乳酸脱氢酶
分类号:
R-332
DOI:
10.3969/j.issn.1671-7414.2015.05.017
文献标志码:
A
摘要:
目的探讨创伤性失血性休克(traumatic hemorrhagic shock，THS)中血浆乳酸脱氢酶(lactate dehydrogenase，LDH)动态变化及其临床价值。方法选择无特定病原体成年大鼠19只，通过急性失血方式建立收缩压下降曲线，形成THS动物模型，测定收缩压基础值(T1)，记录收缩压降至基础值3/4(T2)，基础值1/2(T3)，基础值2/5(T4)，基础值1/5(T5)，10 mmHg(T6)时失血量，采用酶联免疫吸附分析检测各时间点血浆LDH含量水平。结果T2～T3及T4～T5时间段组内差异有统计学意义(P<0.05)，T3～T4及T5～T6时间段组内差异无统计学意义(P>0.05)，T2～T3时间段组内LDH含量水平显著性升高，T4～T5时间段组内LDH含量水平显著性下降。结论选择收缩压下降曲线建立THS模型评估失血量变化，结合血浆LDH含量水平动态监测，可对THS过程中组织细胞损伤程度进行有效评估，LDH含量水平可作为判断组织细胞损伤反应的血清学指标。
Abstract:
ObjectiveTo explore the dynamic changes in the plasma levels of lactate dehydrogenase(LDH) with traumatic hemorrhagic shock(THS)，MethodsThe model of the THS with gradient decerease in the systolic blood pressure was eatablished in 19 rats without specific pathogen．The arterial blood during the different phase of shock，i．e．when the systolic pressures were 3/4，2/1，5/2 of the basis systolic blood pressure，5/5 of the basic systolic blood pressure and 10 mmHg，was collected and LDH levels in the plasma were determined by the double antibody sandwich method of enzyme-labeled immunosorbentassay(ELISA) and analyzed．ResultsThere were significant differences in plasma levels of LDH between the rats in T2～T3 and T4～T5 period of time (P<0.05)，and there was no significant differences in plasma levels of LDH between the T3～T4 and T5～T6 period of time(P>0.05)，and LDH levels in T2～T3 period of time　were significant increased with the extension of time，with LDH levels in T4～T5 period of time were significant falling with the extension of time．ConclusionThe animal model of THS is related to the systolic pressure during the shock，may be helpful to assessment changes in blood loss．The dynamic observation of the plasma levels of LDH which is a good index of the damage to the tissue cells，may be helpful to assessment of the THS is related to the degree of damage to the tissue cells．LDH levels can be serological indicators used as tissue cells damage reaction．

参考文献/References:

［1］李维礁，陈媛，朱玮亚，等．家兔急性失血性休克及分组治疗的血压、微循环同步观测［J］．昆明医学院学报，2011，32(5)：20-23． Li WJ，Chen Y，Zhu WY，et al．Simultaneous observation of blood pressure and microcirculation of rabbits with acute hemorrhagic shock after treatment［J］．Journal of Kunming Medical University，2011，32(5)：20-23．
[2]刘卫华，黄群英，庞振瑶．高血压患者肾微循环功能障碍与冠状动脉病变相关研究［J］．陕西医学杂志，2010，39(12)：1611-1613． Liu WH，Huang QY，Pang ZY．The relationship of renal microcirculation dysfunction and severity of coronary artery stenosis in essntial hypertension patients［J］．Shaanxi Medical Journal，2010，39(12)：1611-1613．
[3]王艳，郑宁，刁波，等．谷氨酰胺对隐匿性压疮老年大鼠SOD，CPK及LDH的影响［J］．华南国防医药杂志，2013，27(1)：9-12． Wang Y，Zheng N，Diao B，et al．Influence of glutamine of superoxidedismutase，creatine phosphockinase and lactate dehydrogenase in elderly rats with ocult pressure sore［J］．Mil Med J South Chin，2013，27(1)：9-12．
[4]李大鹏，李震，王刚，等．创伤性失血性休克发生过程中收缩压与失血量的相关性研究［J］．医学信息，2013，26(11)：150-151． Li DP，Li Z，Wang G，et al．Study on the correlative for systolic blood pressure and blood loss volume in traumatic hemorrhagic shock［J］．Medical Information，2013，26(11)：150-151．
[5]于天瑜．102例限制性液体复苏治疗创伤失血性休克的临床研究［J］．吉林医学，2013，34(21)：4252． Yu TY．102 cases of limited fluid resuscitation clinical research for the treatment of traumatic uncontrolled hemorrhagic shock［J］．Jilin Medical Journal，2013，34(21)：4252．
[6]侯丹，谭雯．创伤性肝破裂合并失血性休克不同早期液体复苏方法疗效比较［J］．中国老年学杂志，2012，32(21)：4819-4820． Hou D，Tan W．Traumatic hepatic rupture complicating uncontrolled hemorrhagic shock different early fluid resuscitation method curative effect comparison［J］．Chinese Journal of Gerontology，2012，32(21)：4819-4820．
[7]程卫东，孙明寅．限制性液体复苏在创伤失血性休克急救中的应用［J］．安徽医学，2013，17(4)：662-663． Cheng WD，Sun MY．Restrictive liquid recovery application in uncontrolled hemorrhagic shock trauma first aid［J］．Anhui Medical and Pharmaceutical Journal，2013，17(4)：662-663．

相似文献/References:

[1]李大鹏,李 震,王 刚,等.创伤性失血性休克大鼠血浆 血栓调节蛋白的动态测定及价值[J].现代检验医学杂志,2015,30(01):116.[doi:10.3969/j.issn.1671-7414.2015.01.033]
　LI Da-peng,LI Zhen,WANG Gang,et al.Dynamic Observation and Value of Plasma Level of Thrombomodulin in Rats with Traumatic Hemorrhagic Shock[J].Journal of Modern Laboratory Medicine,2015,30(05):116.[doi:10.3969/j.issn.1671-7414.2015.01.033]

备注/Memo

备注/Memo:
基金项目：军队“十二五”后勤科研计划项目(课题编号：CWS11J297)。 作者简介：李大鹏(1964-)，男，副主任技师，研究方向：医学实验，Tel：13583848186，E-mail：Lidapeng1964@126.com。 通讯作者：王永清，男，Tel：15653808808，E-mail：WYQ96307@126.com。
更新日期/Last Update: 1900-01-01