[1]张阳东a,陈洁b,楚瑞雪,等.2型糖尿病患者低密度脂蛋白水平及其受体基因表达水平研究[J].现代检验医学杂志,2015,30(05):97-98.[doi:10.3969/j.issn.1671-7414.2015.05.029]
　ZHANG Yang-donga,CHEN Jieb,CHU Rui-xue,et al.Study on Low-density Lipoprotein Levels and It’s Receptor Genes Expression Levels in Type 2 Diabetes Mellitus Patients[J].Journal of Modern Laboratory Medicine,2015,30(05):97-98.[doi:10.3969/j.issn.1671-7414.2015.05.029]
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2型糖尿病患者低密度脂蛋白水平及其受体基因表达水平研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年05期
页码:
97-98
栏目:
论著
出版日期:
2015-12-10

文章信息/Info

Title:
Study on Low-density Lipoprotein Levels and It’s Receptor Genes Expression Levels in Type 2 Diabetes Mellitus Patients
作者:
张阳东1a; 陈洁1b; 楚瑞雪2; 王利营1a; 云洁1a; 安娟1a; 方靓1a
1.第二炮兵总医院 a.特勤医学科；b.护理部，北京100088；2.第二炮兵礼士路门诊部，北京100820
Author(s):
ZHANG Yang-dong1a; CHEN Jie1b; CHU Rui-xue2; WANG Li-ying1a; YUN Jie1a;  AN Juan1a; FANG Liang1a
1a.Special Service Medicine Department； 1b.Nursing Department，the General Hospital of the Second Artillery， Beijing 100088，China；2.Lishilu Clinic of the Second Artillery，Beijing 100820，China
关键词:
2型糖尿病; 低密度脂蛋白; 低密度脂蛋白受体
分类号:
R587.1；Q786
DOI:
10.3969/j.issn.1671-7414.2015.05.029
文献标志码:
A
摘要:
目的观察低密度脂蛋白(LDL)水平和低密度脂蛋白受体(LDLR)基因表达水平与2型糖尿病(T2DM)的相关性。方法提取2013年3月～7月就诊于第二炮兵总医院的95例T2DM患者和113例健康对照者外周血白细胞RNA，应用荧光标记技术检测LDLR的基因表达水平；采用全自动生化分析仪及酶法检测血清低密度脂蛋白-胆固醇水平(LDL-C)。结果T2DM组LDLR基因表达水平为1.988±1.158，正常对照组为2.601±2.350，差异有统计学意义(P=0.016)；T2DM组LDL-C为2.828±0.806，正常对照组为2.889±0.820，差异无统计学意义(P=0.591)，两组的LDLR基因表达和LDL-C水平间无相关性(r=0.070，P=0.503)。结论受体途径可能不是T2DM患者LDL的主要代谢途径，LDLR基因表达水平可作为T2DM的诊断指标。
Abstract:
ObjectiveTo investigate the association among low-density lipoprotein (LDL) levels and low-density lipoprotein receptor (LDLR) gene expression levels and type 2 diabetes mellitus (T2DM)．MethodsExtracted RNA samples of peripheral blood white cells of 95 patients with T2DM and 113 healthy individuals in March 2013 to July 2013 from the general hospital of the second artillery．LDLR gene expression levels were analysed with fluorescence labeled technology，and the levels of low-density lipoprotein-cholesterol (LDL-C) in serum were detected by automatic biochemical modular system with enzyme．ResultsLDLR gene expression levels of T2DM group were 1.988±1.158，and that of control group were 2.601±2.350．It had statistical difference between the two groups (P=0.016)．The serum LDL-C levels of T2DM group were 2.828±0.806，and that of control group were 2.889±0.820．It had not statistical difference between the two groups (P=0.591)．The levels of LDLR gene expression of the two groups were not associated with LDL-C (r=0.07，P>0.05)．ConclusionReceptor method may not be the main metabolic method of LDL in T2DM patients．LDLR gene expression level could be regarded as a diagnostic marker of T2DM．

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备注/Memo

备注/Memo:
作者简介：张阳东(1970-)，男，医学博士，主任技师，研究方向：临床生物化学与分子生物学诊断，Tel：13671041699，E-mail：zhangyd262@sohu.com。
更新日期/Last Update: 1900-01-01