[1]余清,罗君,蒋维.院内鲍曼不动杆菌耐药谱分析及相关β-内酰胺酶基因分布情况调查[J].现代检验医学杂志,2015,30(06):60-63.[doi:10.3969/j.issn.1671-7414.2015.06.017]
　YU Qing,LUO Jun,JIANG Wei.Study on the Characteristic of Drug Resistance and β-lactamase Genes in Acinetobacter Baumannii[J].Journal of Modern Laboratory Medicine,2015,30(06):60-63.[doi:10.3969/j.issn.1671-7414.2015.06.017]
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院内鲍曼不动杆菌耐药谱分析及相关β-内酰胺酶基因分布情况调查()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年06期
页码:
60-63
栏目:
论著
出版日期:
2015-12-30

文章信息/Info

Title:
Study on the Characteristic of Drug Resistance and β-lactamase Genes in Acinetobacter Baumannii
作者:
余清; 罗君; 蒋维
南京中医药大学附属医院检验科，南京210029
Author(s):
YU Qing; LUO Jun; JIANG Wei
Department of Clinical Laboratory， Affiliated Hospital of Nanjing University of TCM，Nanjing 210029，China
关键词:
鲍曼不动杆菌; β-内酰胺酶基因; 抗药性
分类号:
R378；Q786
DOI:
10.3969/j.issn.1671-7414.2015.06.017
文献标志码:
A
摘要:
目的对南京中医药大学附属医院院内分离出来的鲍曼不动杆菌进行各类β-内酰胺酶基因的检测，探讨这些基因的分布特点，研究鲍曼不动杆菌的耐药机制。方法收集2014年1月～12月期间医院内分离的200株鲍曼不动杆菌，最低抑菌浓度(MIC)法测定其对抗生素的敏感性；PCR法检测A类β-内酰胺酶基因SHV，GES，TEM；B类β-内酰胺酶基因VIM，IMP；C类β-内酰胺酶基因AmpC和D类β-内酰胺酶基因OXA-51，OXA-23，OXA-24，OXA-58在菌株中的分布。结果200株鲍曼不动杆菌中116株为多重耐药株，其对常用抗生素的耐药率超过70%；其中52株携带TEM，携带率为26.0％，135株携带SHV，携带率为67.0％，7株检出GES，携带率为3.5%；170株携带VIM-1，携带率为85.0%，12株检出IMP-1，携带率为6.0％；150株携带AmpC，携带率为75.0%；190株携带OXA-51，携带率为95.0%，165株携带OXA-23，携带率为82.5%，2株携带OXA-58，携带率为1.0％，所有菌株未检出OXA-24。结论携带β-内酰胺酶基因是鲍曼不动杆菌的主要耐药机制之一，携带率最高的基因为OXA-51，OXA-23，AmpC，VIM-1和SHV，携带率均超过60％，说明鲍曼不动杆菌的耐药是A，B，C，D四类β-内酰胺酶基因协同作用的结果。
Abstract:
ObjectiveTo study the β-lactamase genes in Acinetobacter baumannii (Ab) acquired in Affiliated Hospital of Nanjing University of TCM．MethodsCollected 200 Ab isolates from January 2014 to December 2014．Antibiotic susceptibility were identified by MIC，then β-lactamase genes including TEM，SHV，GES，IMP-1，VIM-1，AmpC，OXA-51，OXA-23，OXA-24 and OXA-58 were detected by PCR．ResultsThere were 116 multidrug resistant Ab in all isolates．The rate of drug resistance to common antibiotics of mutidrug-resistant Ab was more than 70%．Fifty-two isolates (26.0%) were positive for TEM，135 (67.0%) were positive for SHV，7 (3.5%) were positive for GES，170 (85%) were positive for VIM-1 while 12 (6.0%) for IMP-1，150 isolates (75.0%) were detected carrying AmpC，190 (95.0%) were positive for OXA-51，165 (82.5%) were positive for OXA-23，only 2 (1.0%) were positive for OXA-58．OXA-24 was not detected in any of the isolates．ConclusionCarrying β-lactamase genes may be one of the important mechanisms of drug resistance of Ab．OXA-51，OXA-23，AmpC，VIM-1 and SHV were the most frequent genes．The carrying rates of all these genes were over 60.0%．Results showed that the drug resistance may be the result of synergistic effect of multiple β-lactamase genes．

参考文献/References:

［1］Villar M，Cano ME，Gato E，et al．Epidemiologic and clinical impact of Acinetobacter baumannii colonization and infection：a reappraisal［J］．Medicine(Baltimore)，2014，93(5)：202-210．
[2]Dijkshoorn L，Nemec A，Seifert H．An increasing thr-eat in hospitals：multidrug-resistant Acinetobacter baumannii［J］．Nat Rev Microbiol，2007，5(12)：939-951．
[3]Güven T，Yilmaz G，Güner HR，et al．Increasing resistance of nosocomial Acinetobacter baumannii：are we going to be defeated?［J］．Turk J Med Sci，2014，44(1)：73-78．
[4]叶应妩，王毓三，申子瑜．全国临床检验操作规程［S］．3版．南京：东南大学出版社，2006. Ye YW，Wang YS，Shen ZY．National Guide to Clinical Laboratory Procedures［S］．3th Ed.Nanjing:Southeast University Press，2006．
[5]Clinical and Laboratory Standard Institute．Performance standards for antimicrobial susceptibility testing：seventeenth informational supplement 7［S］．Wayne：PA，CLSI M100-S1，2012．
[6]赵苏瑛，张轶恺，吴倩，等．泛耐药鲍曼不动杆菌耐药基因的检测及其同源性分析［J］．现代检验医学杂志，2014，29(3)：22-26． Zhao SY，Zhang YK，Wu Q，et al．Study on drug resistant-related genes and analysis of homology in pandrug-resistant Acinetobacter baumannii［J］．Journal of Modern Laboratory Medcine，2014，29(3)：22-26．
[7]杜明梅，邢玉斌，邓春燕，等．耐碳青霉烯类抗菌药物鲍氏不动杆菌医院感染暴发与控制［J］．中华医院感染学杂志，2010，20(10)：1462-1464． Du MM，Xing YB，Deng CY，et al．Nosocomial outbreak of infection with carbapenem-resistant Acinetobacter baumannii in ICU and its successful control［J］．Chinese Journal of Nosocomiology，2010，20(10)：1462-1464．
[8]胡付品，朱德妹，汪复，等．2013年中国CHINET细菌耐药性检测［J］．中国感染与化疗杂志，2014，14(5)：369-378． Hu FP，Zhu DM，Wang F，et al．CHINET 2013 surveillance of bacterial resistance in China［J］．Chinese Journal of Infection and Chemotherapy，2014，14(5)：369-378．
[9]陈贤君，张亚琼，郑蓓佳，等．泛耐药鲍氏不动杆菌β-内酰胺酶基因、膜孔蛋白基因及外排泵基因研究［J］．中华医院感染学杂志，2011，21(22)：4650-4653． Chen XJ，Zhang YQ，Zheng BJ，et al．Investigation of β-lactamase genes，outer membrane porin gene and drug efflux genes in pandrug-resistant Acinetobacter baumannii［J］．Chinese Journal of Nosocomiology，2011，21(22)：4650-4653．
[10]Peleg AY，Seifert H，Paterson DL．Acinetobacter ba-umannii：emergence of a successful pathogen［J］．Clin Microbiol，2008，21(3)：838-582．
[11]Park YS，Lee H，Lee KS．Extensively drug-resistant Acinetobacter baumannii：risk factors for acquisition and prevalent OXA-type carbapenemases-a multicentre study［J］．International Journal of Antimicrobial Agents，2010，36(5)：430-435．
[12]李永丽，应春妹．耐碳青霉烯类鲍曼不动杆菌分子流行病学及耐药机制研究［J］．上海交通大学学报：医学版，2012，32(11)：1430-1435． Li YL，Ying CM．Research on molecular epidemiology and resistance mechanism of carbapenems resistant Acinetobacter baumannii［J］．Journal of Shanghai Jiaotong University(Medical Science)，2012，32(11)：1430-1435．
[13]Merkier AK Centrón D．blaOXA-51-type beta-lactamase genes are ubiquitous and vary within a strain in Acinetobacter baumannii［J］．Int J Antimicrob Agents，2006，28(2)：110-113．
[14]Mugnier PD，Poirel L，Naas T，et al．Worldwide dissemination of the blaOXA-23 carbapenemase gene of Acinetobacter baumannii［J］．Emerging Infectious Diseases，2010，16(1)：35-40．
[15]金辉，徐小敏，糜祖煌，等．鲍曼不动杆菌的耐药基因分型与脉冲场凝胶电泳分型的比较［J］．环境与职业医学，2010，27(4)：219-221，225． Jin H，Xu XM，Mi ZH，et al．Comparison of drug resistant genes typing and pulsed-field gel electrophoresis typing for Acinetobacter baumannii［J］．Journal of Environmental & Occupational Medicine，2010，27(4)：219-221,225．
[16]王丽娟，季萍，张朝霞．乌鲁木齐地区耐碳青霉烯鲍曼不动杆菌碳青霉烯酶基因型及同源性分析［J］．现代检验医学杂志，2011，26(3)：72-75． Wang LJ，Ji P，Zhang ZX．Study on the homology and carbapenemases genotypes of imipenem-resistant Acinetobacter baumannii strains from Urumqi region［J］．Journal of Modern Laboratory Medcine，2011，26(3)：72-75．
[17]Khorsi K，Messai Y，Hamidi M，et al．High prevalence of multidrug-resistance in Acinetobacter baumannii and dissemination of carbapenemase-encoding genes blaOXA-23-like，blaOXA-24-like and blaNDM-1 in Algiers hospitals［J］．Asian Pacific Journal of Tropical Medicine，2015，８(6)：438-446．
[18]Zeka AN，Poirel L，Sipahi OR，et al．GES-type and OXA-23 carbapenemase-producing Acinetobacter baumannii in Turkey［J］．Joumal Antimicrob Chemother，2014，69(4)：1145-1146．

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备注/Memo

备注/Memo:
作者简介：余清(1980-)，女，本科，学士，主管技师，主要研究方向：临床检验诊断学。 通讯作者：蒋维(1972-)，男，副主任技师，E-mail：jwjl1234@163.com
更新日期/Last Update: 1900-01-01