[1]张丽娇,王思明,曾 洁,等.血清不同温育条件对高密度脂蛋白胆固醇的稳定性影响研究[J].现代检验医学杂志,2016,31(01):21-24.[doi:10.3969/j.issn.1671-7414.2016.01.006]
　ZHANG Li-jiao,WANG Si-ming,ZENG Jie,et al.Stability of High-Density Lipoprotein Cholesterol During Serum Incubation[J].Journal of Modern Laboratory Medicine,2016,31(01):21-24.[doi:10.3969/j.issn.1671-7414.2016.01.006]
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血清不同温育条件对高密度脂蛋白胆固醇的稳定性影响研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年01期
页码:
21-24
栏目:
论著
出版日期:
2016-01-25

文章信息/Info

Title:
Stability of High-Density Lipoprotein Cholesterol During Serum Incubation
作者:
张丽娇1; 王思明1; 曾 洁2; 杨睿悦1; 李红霞1; 王 抒1; 董 军1; 陈文祥1; 2
1.卫生部北京医院/卫生部北京老年医学研究所卫生部老年医学重点实验室,北京 100730; 2.卫生部临床检验中心,北京 100730
Author(s):
ZHANG Li-jiao1; WANG Si-ming1; ZENG Jie2; YANGRui-yue1; LI Hong-xia1; WANG Shu1;  DONG Jun1; CHEN Wen-xiang1; 2
1.the Key Laboratory ofGeriatrics, Beijing Hospital & Beijing Institute of Geriatrics,Beijing 100730,China; 2.National Center for Clinical Laboratories,Beijing 100730,China
关键词:
高密度脂蛋白;  胆固醇;  稳定性;  高效液相色谱;  血清温育
分类号:
R446.112
DOI:
10.3969/j.issn.1671-7414.2016.01.006
文献标志码:
A
摘要:
目的 探讨血清高密度脂蛋白胆固醇(HDL-C)在不同温度、时间下的储存稳定性。方法 2015年5月自卫生部北京医院征集健康志愿者10人(男性4人,女性6人,年龄24～59岁),将收集的血清在4℃温育24 h,25℃温育0,1,8和24 h[分别含和不含卵磷脂胆固醇酰基转移酶(LCAT)抑制剂],HPLC法测定血清总胆固醇(TC),总游离胆固醇(TFC),HDL-C以及HDL游离胆固醇(HDL-FC)。使用微软EXCEL软件进行结果分析。结果 血清在4℃温育24 h,造成HDL-FC和HDL-C变化(平均为-6.91%和-2.17%); 25℃温育24 h造成TFC,HDL-FC及HDL-C的变化(平均为-13.70%,-25.88%和-1.53%); 25℃下LCAT抑制剂完全抑制TFC的降低、部分抑制HDL-FC的降低,使HDL-C降低幅度更大。结论 血清贮存可造成胆固醇变化,这种变化取决于LCAT,胆固醇酯转移蛋白(CETP)的活性以及FC在脂蛋白之间的转移。因此,为提高血脂测定结果的准确性,应尽量减少不必要的血清贮存。
Abstract:
Objective To examine the stability of high-density lipoprotein cholesterol(HDL-C)during serum incubation at different temperature and time periods.Methods Ten healthy volunteers(4 males and 6 females,aged 24 to 59 years)from Beijing Hospital were recruitedin May 2015. Fasting venous blood samples were collected and centrifuged to separate the sera.Serum samples were incubated at 4℃ for 24 h,25℃for 0,1,8 and 24 h(with or without an LCAT inhibitor).Serum total cholesterol(TC),totalfree cholesterol(TFC)HDL-C and HDL-FC were measured by the HPLC Method.Results HDL-FC and HDL-C changed -6.91% and -2.17% during serum incubation at 4℃for 24h.TFC,HDL-FC and HDL-C changed significantly(averaged -13.70%,-25.88% and -1.53% respectively)during serum incubation at 25℃ for 24 h,in which the decrease of TFC and HDL-FC were inhibited by theaddition of the LCAT inhibitor.The decrease of HDL-C was even higher in the presence of the LCAT inhibitor.Conclusion Serum TFC,HDL-C and HDL-FC levels changed during serum incubations,which were caused by theLCAT and CETP activities and the transfer of cholesterol among lipoproteins.Foraccurate measurement of serum HDL-C,prolonged serum storage should be avoidedin clinical laboratories.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81472035,81171647)。 作者简介:张丽娇(1987-),女,硕士研究生,研究方向:脂代谢与血脂测定标准化,Tel:18610694050,E-mail:693230455@qq.com。 通讯作者:董 军,研究员,E-mail:jun dong@263,net。
更新日期/Last Update: 2016-01-20