[1]杨文丽,申 旺,黄秀芳,等.磷脂酰肌醇蛋白聚糖3在肝细胞癌组织中的表达及临床意义[J].现代检验医学杂志,2016,31(01):73-76.[doi:10.3969/j.issn.1671-7414.2016.01.021]
　YANG Wen-li,SHEN Wang,HUANG Xiu-fang,et al.Expression and Clinical Significance of Glypican-3 in Hepatocellular Carcinoma Tissues[J].Journal of Modern Laboratory Medicine,2016,31(01):73-76.[doi:10.3969/j.issn.1671-7414.2016.01.021]
点击复制

磷脂酰肌醇蛋白聚糖3在肝细胞癌组织中的表达及临床意义()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年01期
页码:
73-76
栏目:
论著
出版日期:
2016-01-25

文章信息/Info

Title:
Expression and Clinical Significance of Glypican-3 in Hepatocellular Carcinoma Tissues
作者:
杨文丽1; 申 旺2; 黄秀芳1; 李 青1; 刘琼茹1
1.中山大学附属江门中心医院病理科, 广东江门 529030; 2.江门市五邑中医院检验科,广东江门 529000
Author(s):
YANG Wen-li1; SHEN Wang2; HUANG Xiu-fang1; LI Qing1; LIU Qiong-ru1
1.Department of Pathology Laboratory,Jiangmen Central HospitalAffiliated to Zhongshan Medical University,Guangdong Jiangmen 529030,China; 2.Department of Clinical Laboratory, Wuyi Traditional Chinese Medicine Hospital,Guangdong Jiangmen 529000,China
关键词:
磷脂酰肌醇蛋白聚糖3;  肝细胞癌;  免疫组织化学
分类号:
R735.7; R730.43
DOI:
10.3969/j.issn.1671-7414.2016.01.021
文献标志码:
A
摘要:
目的 探讨磷脂酰肌醇蛋白聚糖3(Glypican-3,GPC3)在肝细胞癌(hepatocellular carcinoma,HCC)组织中的表达及临床意义。方法 采用免疫组织化学法检测2011年6月～2015年3月期间的54例HCC组织、46例癌旁组织、22例肝硬化组织、12例正常肝组织中的GPC3表达情况,分析GPC3表达与临床和病理因素的相关性。结果 正常肝组织中不表达GPC3蛋白,肝癌组织GPC3评分(7.39±3.64)和阳性率(81.48%)均显著高于癌旁组织(1.15±0.99,13.04%)、肝硬化组织(0.32±0.56,4.54%),差异有统计学意义(P<0.05); 随着肝癌临床分期的增高,GPC3评分逐步升高,Ⅲ/Ⅳ期(10.05±3.59)与Ⅰ期(4.31±3.41)、Ⅱ期(7.14±3.63)比较,差异有统计学意义(P<0.05),GPC3阳性率也升高(Ⅰ期69.23%,Ⅱ期81.81%,Ⅲ/Ⅳ期89.47%),但差异无统计学意义(P>0.05); 肝癌组织GPC3阳性率与性别、年龄、血清HBsAg、甲胎蛋白(alpha-fetoprotein,AFP)、肿瘤直径、转移情况、临床分期无关(P>0.05),与是否并发肝硬化相关(P<0.05),GPC3高表达率与血清AFP、肿瘤直径、是否并发肝硬化、转移情况、临床分期均相关(P<0.05)。结论 GPC3在HCC的诊断方面具有重要的临床意义,其表达水平与HCC的病情进展相关。
Abstract:
Objective To study the expression and the clinical significance of glyican-3(GPC3)in hepatocellular carcinoma(HCC)tissues.Methods Immunohistochemical method was performed to evaluate the expression of GPC3 in 54 cases of HCC tissues,46 cases of para-carcinoma tissues,22 cases of cirrhosis tissues,12 cases of normal liver tissues,the correlation between expression levels of GPC3 and clinicopathologic factors was analyzed.Results GPC3 protein was not expressed in normal livers tissues,the levels of GPC3(7.39±3.64)and the positive rate(81.48%)in HCC tissues were significantly higher than para-carcinoma tissues(1.15±0.99,13.04%),cirrhosis tissues(0.32±0.56,4.54%),the differencewas statistically significant(P<0.05); with the increase of clinical stageofHCC,the levels of GPC3 were increased,stage Ⅲ/Ⅳ(10.05±3.59)comparedwith stage Ⅰ(4.31±3.41),stage Ⅱ(7.14±3.63),and the difference was statistically significant(P<0.05).The positive rate of GPC3 was also increased(stage Ⅰ 69.23%,stage Ⅱ 81.81%,stage Ⅲ/Ⅳ 89.47%)and the differencewas not statistically significant(P>0.05); the positive rate in HCC tissues was independent of sex,age,serum HBsAg,alpha-fetoprotein(AFP),tumor diameter,metastasis,clinical stage(P>0.05),only related to the cirrhosis(P<0.05).The high expression rate of GPC3 in HCC tissues was correlated with AFP,tumor diameter,cirrhosis,metastasis,clinical stage(P<0.05).Conclusion GPC3 has important clinical significance in the diagnosis of HCC,the expression level of GPC3 associated with the progression ofHCC.

参考文献/References:

[1] 申 旺,杨文丽,叶丽燕,等.血清唾液酸在原发性肝癌和转移性肝癌诊断中的临床价值[J].现代检验医学杂志,2015,30(1):140-142. Shen W,Yang WL,Ye LY,et al.Clinical value of serum sialic acid detection for diagnosis in primary hepatic carcinoma and metastatic hepatic carcinoma[J].J Mod Lab Med,2015,30(1):140-142.
[2] Akahoshi H, Taura N, Ichikawa T,et al.Differences in prognostic factors according to viral status in patients with hepatocellular carcinoma[J].Oncol Rep,2010,23(5):1317-1323.
[3] Yu J,Ma Q,Zhang B,et al.Clinical application of specific antibody against glypican-3 for hepatocellular carcinoma diagnosis[J].Sci China Life Sci,2013,56(3):234-239.
[4] Tangkijvanich P,Chanmee T,Komtong S,et al.Diagnostic role of serum glypican-3 in differentiating hepatocellular carcinoma from non-malignant chronic liver disease and other liver cancers[J].J Gastroenterol Hepatol,2010,25(1):129-137.
[5] 杨 龙,刘剑勇.GPC3与肝癌的研究进展[J].中国癌症防治杂志,2010,2(2):146-148. Yang L,Liu JY.Research progress of GPC3 and hepatocellular carcinoma[J].Chin J Oncol Prev Treat,2010,2(2):146-148.
[6] 康凯夫,张 鑫,陈小伍,等.Glypican-3在原发性肝细胞性肝癌中的表达及其临床意义[J].广东医学,2009,30(10):1480-1482. Kang KF,Zhang X,Chen XW,et al.The expression and clinic significance of glypican-3 protein in primary human hepatocellular carcinoma[J].Guangdong Med J,2009,30(10):1480-1482.
[7] Yao M, Yao DF, Bian YZ,et al.Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma[J].Hepatobiliary Pancreat Dis Int,2011,10(3):289-294.
[8] 王 涛,王风梅,高英堂,等.磷脂酰肌醇蛋白聚糖3在肝穿刺活检标本中鉴别诊断的意义[J].世界华人消化杂志,2011,19(7):693-699. Wang T,Wang FM,Gao YT,et al.Significance of GPC3 expression in liver biopsy specimens for differential diagnosis of liver disease[J].World Chin J Digest,2011,19(7):693-699.
[9] 范公忍,蔺会云,李 琳,等.磷脂酰肌醇蛋白聚糖-3在原发性肝癌中的表达及临床意义[J].北京医学,2013,35(6):423-426. Fan GR,Lin HY,Li L,et al.Expression and clinical significance ofglypican-3 in primary hepatocellular carcinoma[J].Beijing Med J,2013,35(6):423-426.
[10] 付顺军,李绍强,林 杰,等.Glypican-3在原发性肝细胞癌中的表达及其对预后的影响[J].中国病理生理杂志,2010,26(12):2351-2357. Fu SJ,Li SQ,Lin J,et al.Expression of glypican-3 in hepatocellular carcinoma and its clinical significance[J]. Chin J Pathophysiol,2010,26(12):2351-2357.

相似文献/References:

[1]张巧娣,谢而付,凌 芸,等.壳多糖酶3样蛋白1与甲胎蛋白在肝细胞癌诊断中的比较[J].现代检验医学杂志,2017,32(01):45.[doi:10.3969/j.issn.1671-7414.2017.01.013]
　ZHANG Qiao-di,XIE Er-fu,LING Yun,et al.Comparative Diagnostic Value of Chitinase 3-Like 1 Protein and AFP in the Diagnosis of Hepatocellular Carcinoma[J].Journal of Modern Laboratory Medicine,2017,32(01):45.[doi:10.3969/j.issn.1671-7414.2017.01.013]

备注/Memo

备注/Memo:
作者简介:杨文丽(1984-),女,本科,主管病理技师,主要研究方向为免疫组化和分子病理学技术,Tel:15876296850,E-mail:yangwenli902@163.com。
更新日期/Last Update: 2016-01-20