[1]刘连庚,姜雪秋,张金蓉,等.皮肤鳞癌组织中E-Cadherin,p14ARF,P16基因启动子甲基化检测及其意义[J].现代检验医学杂志,2016,31(02):15-19.[doi:10.3969/j.issn.1671-7414.2016.02.005]
　LIU Lian-geng,JIANG Xue-qiu,ZHANG Jin-rong,et al.Clinical Significance of Methylation of E-cadherin p14ARF and P16 Gene Promoters in Skin Squamous Cell Carcinoma Tissues[J].Journal of Modern Laboratory Medicine,2016,31(02):15-19.[doi:10.3969/j.issn.1671-7414.2016.02.005]
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皮肤鳞癌组织中E-Cadherin,p14ARF,P16INK4a基因启动子甲基化检测及其意义()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年02期
页码:
15-19
栏目:
论著
出版日期:
2016-06-01

文章信息/Info

Title:
Clinical Significance of Methylation of E-cadherin p14ARF and P16INK4a Gene Promoters in Skin Squamous Cell Carcinoma Tissues
作者:
刘连庚1,姜雪秋2,张金蓉2,王 源3,吴 健4
1.盐城市中医院检验科,江苏盐城 224001; 2.大丰市人民医院,江苏大丰 224100; 3.浙江省中医院,杭州 310000; 4.盐城市第一人民医院,江苏盐城 224005
Author(s):
LIU Lian-geng1,JIANG Xue-qiu2,ZHANG Jin-rong2,WANG Yuan3,WU Jian4
1.Department of Laboratory Medicine,the Traditional Chinese Medicine Hospital of Yancheng City,Jiangsu Yancheng 224000,China; 2.Department of Laboratory Medicine,Dafeng People's Hospital,Jiangsu Dafeng 224100,China; 3.Department of Laboratory Medicine,th
关键词:
皮肤鳞状细胞癌;  E-cadherin;  p14ARF;  P16INK4a;  DNA甲基化
分类号:
R739.5; Q786
DOI:
10.3969/j.issn.1671-7414.2016.02.005
文献标志码:
A
摘要:
目的 通过检测皮肤鳞癌组织及正常皮肤组织中E-cadherin,p14ARF,P16INK4a基因启动子甲基化的发生情况,探讨E-cadherin,p14ARF和P16INK4a基因启动子甲基化在皮肤鳞癌发生发展中的作用。方法 采用酚-氯仿法提取30例皮肤鳞癌组织及正常皮肤组织的基因组DNA,应用甲基化特异性PCR对所提DNA进行E-cadherin,p14ARF和P16INK4a基因甲基化检测。结果癌组织中E-cadherin,p14ARF和P16INK4a基因启动子甲基化的阳性率分别为36.7%(11/30),60.0%(18/30)和53.3%(16/30),而正常皮肤组织中相应的3个基因的甲基化率分别为10.0%(3/30),6.7%(2/30)和6.7%(2/30),均显著低于癌组织。结论 皮肤鳞癌中P16INK4a基因启动子甲基化与正常皮肤组织有明显差异,且在低分化癌中更多见; E-cadherin基因启动子甲基化与正常皮肤比较有显著差异,并且有淋巴结转移多见的显著特征,皮肤鳞癌中p14ARF基因甲基化发生率明显高于正常皮肤,且与分化程度、淋巴结转移和临床分期有关系,同时p14ARF,E-Cadherin,p16INK4a三种基因甲基化存在正相关关系,这3个基因的甲基化位点与皮肤鳞癌密切相关。
Abstract:
Objective To discuss the function of E-cadherin,p14ARF and P16INK4a gene promoter methylution in the development of skin cancer by examining occurence conditions of E-cadherin,p14ARF and P16INK4a gene promoter methylution in the skin squamous carcinoma tissues and normal skin tissues.Methods Skin squamous cell carcinoma specimens were collected from 30 patients and normal skin tissues were collected from 30 individuals as controls.Promoter methylation was detected for E-cadherin,p14ARF and P16INK4a by methylation-specific PCR.Correlations between E-cadherin,p14ARF or P16INK4a methylation and clinicopathological parameters were analyzed by the Spearman rank test.Results Methylation of E-cadherin(36.7%),p14ARF(60.0%)and P16INK4a(53.3%)was significantly more common in skin squamous cell carcinoma than in normal skin tissue(10.0%,6.7% and 6.7%,respectively P<0.05).Conclusion E-cadherin,p14ARF and P16INK4a methylation were positively correlated within skin squamous cell carcinoma.Furthermore,methylation of these gene promoters in skin squamous cell carcinoma was correlated with differentiation,lymph node metastasis,and clinical stage(P<0.05).Aberrant methylation in promoters of E-cadherin,p14ARF and P16INK4a may promote occurrence and progression of skin squamous cell carcinoma.

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备注/Memo

备注/Memo:
基金项目:2015年江苏省药学会-百特生物药学基金(201542),浙江省中医药科技计划项目(2015ZB054)。 作者简介:刘连庚(1970-),男,本科,副主任检验师,主要从事肿瘤诊断研究,E-mail:wujianglinxing@163.com。 通讯作者:吴 健(1979-),男,硕士,主管检验师,主要从事肿瘤表观遗传研究,E-mail:piaoxue1982717@sina.com。
更新日期/Last Update: 2016-02-20