[1]杨 健,王西娣,陈美玲,等.2013～2015年陕西地区结核分枝杆菌对利福平耐药性及rpoB基因突变的相关研究[J].现代检验医学杂志,2016,31(02):53-55,59.[doi:10.3969/j.issn.1671-7414.2016.02.016]
　YANG Jian,WANG Xi-di,CHEN Mei-ling,et al.Related Research on Resistance of Mycobacterium Tuberculosis to Rifampin and rpoB Gene Mutations in Shaanxi from 2013 to 2015[J].Journal of Modern Laboratory Medicine,2016,31(02):53-55,59.[doi:10.3969/j.issn.1671-7414.2016.02.016]
点击复制

2013～2015年陕西地区结核分枝杆菌对利福平耐药性及rpoB基因突变的相关研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年02期
页码:
53-55,59
栏目:
论著
出版日期:
2016-06-01

文章信息/Info

Title:
Related Research on Resistance of Mycobacterium Tuberculosis to Rifampin and rpoB Gene Mutations in Shaanxi from 2013 to 2015
作者:
杨 健; 王西娣; 陈美玲; 李 妍; 王 蕊
陕西省结核病防治研究所结核病参比实验室,西安 710048
Author(s):
YANG Jian; WANG Xi-di; CHEN Mei-ling; LI Yan; WANG Rui
Tuberculosis Reference Laboratory of Shaanxi Provincial Institute for Tuberculosis Control and Prevention,Xi'an 710048,China
关键词:
结核分枝杆菌;  利福平;  耐药;  rpoB基因
分类号:
R378.911; Q754
DOI:
10.3969/j.issn.1671-7414.2016.02.016
文献标志码:
A
摘要:
目的 分析陕西地区结核分枝杆菌(MTB)对利福平(RFP)耐药性及rpoB基因突变情况。方法 收集2013～2015年陕西省结核病防治研究所和陕西省结核病防治院怀疑为肺结核的患者标本3 739例,采用利福平耐药实时荧光定量核酸扩增检测技术(Xpert MTB/RIF)对其进行RFP耐药性及rpoB基因突变检测,选取检测为MTB阳性并诊断为结核病患者的结果1 506例进行RFP耐药性及rpoB基因突变情况分析,采用SPSS18.0软件的卡方检验进行统计学分析P<0.05,差异有统计学意义。结果 2013 ～2015年RFP耐药率分别为17.0%,14.1%和12.1%,其中2013年RFP耐药率显著高于2015年,耐药率差异有统计学意义(χ2=4.843,P=0.028); 三年利福平总耐药率为14.4%,其中肺内组RFP总耐药率15.0%,显著高于肺外组8.1%,差异有统计学意义(χ2=4.713,P=0.03); 四个年龄组RFP耐药率差异有统计学意义(χ2=13.684,P=0.03),其中41～60岁年龄组耐药率显著高于其他年龄组(P<0.05)。利福平耐药rpoB基因突变有一定区域性,突变区域主要为529～533区域并存在多位点突变的现象。结论 陕西地区结核分枝杆菌对RFP耐药水平较高,应加强陕西省的结核病耐药监测。
Abstract:
Objective To analyze rifampin(RFP)resistance of Mycobacterium tuberculosis(MTB)and the mutation of rpoB gene in Shaanxiarea.Methods Collected 3 739 specimens of suspected pulmonary tuberculosis patients in Shaanxi Provincial Institute for Tuberculosis Control and Prevention and Hospital of Shaanxi for Tuberculosis Prevention and Treatment from 2013 to 2015.And all the specimens were detected by the RFP resistance real-time fluorescence quantitative PCR(Xpert MTB/RIF)and selected 1 506 casesof patients with tuberculosis for RFP resistance and rpoB gene mutation analysis.χ2 test was used for statistical analysis(P<0.05)difference wasstatistically significant.Results The resistance rates for RFP from 2013 to 2015 were 17.0%,14.1% and 12.1%.The rate of RFP resistant in 2013 was significantly higher than the rate in 2015 and the difference wasstatistically significant(χ2=4.843,P=0.028).The average rate of RFPresistant was 14.4% in three years.The RFP resistance rate of lung and sputum specimens(15.0%)was significantly higher than that of the specimens outside of the lung(8.1%),and the difference was statistically significant(χ2=4.713,P=0.03).There was no significant difference between male and femalegroups(χ2=1.048,P=0.306).The RFP resistance rate of age from 41 to60 was significantly higher than other age groups,and the difference was statistically significant(χ2=13.684,P=0.03).RFP resistant rpoB gene mutations were regional and region 529～533 had mutation.There were also a number of point mutations.Conclusion The RFP resistance level to Mycobacterium tuberculosis was high in Shaanxi area and the surveillance of drug resistance in tuberculosis should be strengthen in Shaanxi province.

参考文献/References:

[1] World Health Organization.Multidrug and extensively drug-resistant TB(M/XDR-TB):2010 global report on surveillance and response[R/OL].Geneva:World Health Organization,2010.
[2] Drobniewski FA,Wilson SM.The rapid disgnosis of isoniazid and rifampicin resistancein in Mycobacterium tuberculosis:a molecular story[J].Journal of Medical Microbiology,1998,47(3):189-196.
[3] 阴灵芳,孙惠平,周 永,等.耐利福平作为筛选耐多药结核病指标的评估[J].临床肺科杂志,2013,18(9):1656-1658. Yin LF,Sun HP,Zhou Y,et al.Evaluation of rifampincin resistanceas the screening indicator for multi-drug resistant Tuberculosis[J].Journal of Clinical Pulmonary Medicine,2013,18(9):1656-1658.
[4] 王 瑞,杨水云.PCR-SSCP快速检测结核分枝杆菌耐利福平rpoB基因的研究[J].现代检验医学杂志,2008,23(5):18-20. Wang R,Yang SY.Evaluation and comparison research on PCR-SSCP method and DNA sequencing for rapid detection of rpoB mutations in rifampin-esistant Mycobacterium tubeculosis isolates[J].Journal of Modern Laboratory Medicine,2008,23(5):18-20.
[5] 司红艳,李 晴,穆洮俊,等.甘肃省临夏州、甘南州、定西市结核病耐药监测结果分析[J].中国防痨杂志,2009,31(4):206-209. Si HY,Li Q,Mu TJ,et al.Analysis of anti-TB drug resistance surveillance in Linxia,Ganan and Dingxi in Gansu province[J].Chinese Journal ofAntituberculosis,2009,31(4):206-209.
[6] 顾才宏,王 青,王福瑞,等.340例结核病患者一线抗结核药物的耐药监测结果分析[J].宁夏医科大学学报,2013,35(1):74-76. Gu CH,Wang Q,Wang FR,et al.Analysis of drug resistance surveillance in 340 cases of tuberculosis patients with first-line anti tuberculosis drugs[J].Journal of Ningxia Medical University,2013,35(1):74-76.
[7] 王 芝,马如存,卓 玛,等.青海地区结核分枝杆菌检测与KatG,rpoB及gyrA的突变特征分析[J].实用临床医药杂志,2013,17(16):15-17,38. Wang Z,Ma RC,Zhuo M,et al.Detection of Mycobacterium tuberculosis in Qinghai area and analysis of gene mutation characteristics of KatG,rpoB and gyrA[J].Journal of Clinical Medicine in Practice,2013,17(16):15-17,38.
[8] 李君莲,魏继虎,綦迎成,等.新疆地区1998～2008结核分枝杆菌耐药情况分析[J].中华临床医师杂志,2010,4(7):924-927. Li JL,Wei JH,Qi YC,et al.Analysis on drug resistance of Mycobacterium tuberculosis during 1998-2008 in Xinjiang[J].Chin J Clinicians(Electronic Version),2010,4(7):924-927.
[9] 全国第五次结核病流行病学抽样调查指导小组,全国第五次结核病流行病学抽样调查办公室.2010年全国第五次结核病流行病学抽样调查报告[J].中国防痨杂志,2012,34(8):485-508. Technical Guidance Group of the Fitth National TB Epidemiological Survey,the Office of the Fifth National TB Epidemiological survey.The fifth national tuberculosis epidemiological survey in 2010[J].Chinese Journal of Antituberculosis,2012,34(8):485-508.
[10] Cavusoglu C,Turhan A,Akinci P,et al.Evaluation of the genotype MTBDR assay for rapid detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis isolates[J].Journal of Clinical Microbiology,2006,44(7):2338-2342.

相似文献/References:

[1]张 洁,齐红伟,丁北川,等.结核分枝杆菌临床分离株对吡嗪酰胺的药物敏感性分析[J].现代检验医学杂志,2017,32(03):49.[doi:10.3969/j.issn.1671-7414.2017.03.013]
　ZHANG Jie,QI Hong-wei,DING Bei-chuan,et al.Analysis on Pyrazinamide Drug Susceptibility of Mycobacterium Tuberculosis Clinical Strains[J].Journal of Modern Laboratory Medicine,2017,32(02):49.[doi:10.3969/j.issn.1671-7414.2017.03.013]
[2]吴晓康,周维肖,冯 楠,等.影响TB-IGRA检测结果的因素分析及应对措施[J].现代检验医学杂志,2018,33(04):154.[doi:10.3969/j.issn.1671-7414.2018.04.043]
　WU Xiao-kang,ZHOU Wei-xiao,FENG Nan,et al.Analysis of Factors Influencing TB-IGRA Detection Results and Solutions[J].Journal of Modern Laboratory Medicine,2018,33(02):154.[doi:10.3969/j.issn.1671-7414.2018.04.043]
[3]谭玉华,朱应竹,江 燚,等.时间分辨免疫荧光法检测结核感染T细胞释放γ-干扰素的方法建立及初步临床应用[J].现代检验医学杂志,2018,33(06):21.[doi:10.3969/j.issn.1671-7414.2018.06.006]
　TAN Yu-hua,ZHU Ying-zhu,JIANG Yi,et al.Establishment and Preliminary Clinical Application of Interferon Gamma Release Assays for T Cells Infected With Mycobacterium Tuberculosis Based on Time-Resolved Fluoroimmunoassay[J].Journal of Modern Laboratory Medicine,2018,33(02):21.[doi:10.3969/j.issn.1671-7414.2018.06.006]
[4]田斌,文岚,刘燕萍,等.QFT-GIT对儿童肺结核临床诊断价值的meta分析[J].现代检验医学杂志,2019,34(01):89.[doi:10.3969/j.issn.1671-7414.2019.01.023]
　TIAN Bin,WEN Lan,LIU Yan-ping,et al.Clinical Usefulness of QuantiFERON-TB Gold In-Tube(QFT-GIT) Te st for Tuberculosis Diagnosis in Children：A Systematic Review[J].Journal of Modern Laboratory Medicine,2019,34(02):89.[doi:10.3969/j.issn.1671-7414.2019.01.023]
[5]丁　磊,徐俊驰,邱文娜,等.结核分枝杆菌耐药机制和治疗的最新研究进展[J].现代检验医学杂志,2021,36(02):1.[doi:doi:10.3969/j.issn.1671-7414.2021.02.001]
　DING Lei,XU Jun-chi,QIU Wen-na,et al.Latest Research Progress on the Drug Resistance Mechanism and Treatment of Mycobacterium Tuberculosis[J].Journal of Modern Laboratory Medicine,2021,36(02):1.[doi:doi:10.3969/j.issn.1671-7414.2021.02.001]
[6]李　津a,李昌锦b,杨　洁b,等.Geno-typeMTBDRplus分子线性探针杂交技术在结核病诊断与耐药性检测中的应用价值[J].现代检验医学杂志,2021,36(04):119.[doi:10.3969/j.issn.1671-7414.2021.04.025]
　LI-jina,LI Chang-jinb,YANG Jieb,et al.Value of Geno-type MTBDRplus Molecular Linear Probe Hybridization inTuberculosis Diagnosis and Drug Resistance Detection[J].Journal of Modern Laboratory Medicine,2021,36(02):119.[doi:10.3969/j.issn.1671-7414.2021.04.025]

备注/Memo

备注/Memo:
作者简介:杨 健(1986-),男,研究生在读,检验师,从事临床医学检验工作,Tel:029-82231923,E-mail:yangjian198686@126.com。
更新日期/Last Update: 2016-02-20