[1]谢朝云a,b,胡 阳b,等.肠杆菌科细菌环丙沙星耐药株与敏感株耐药性分析[J].现代检验医学杂志,2016,31(02):63-67.[doi:10.3969/j.issn.1671-7414.2016.02.019]
　XIE Zhao-yuna,b,HU Yangb,et al.Enterobacteriaceae Ciprofloxacin Resistant Strains and Sensitive Strains Drug Resistance Analysis[J].Journal of Modern Laboratory Medicine,2016,31(02):63-67.[doi:10.3969/j.issn.1671-7414.2016.02.019]
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肠杆菌科细菌环丙沙星耐药株与敏感株耐药性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年02期
页码:
63-67
栏目:
论著
出版日期:
2016-06-01

文章信息/Info

Title:
Enterobacteriaceae Ciprofloxacin Resistant Strains and Sensitive Strains Drug Resistance Analysis
作者:
谢朝云a; b; 胡 阳b; 杨忠玲c; 孙 静a
贵州医科大学第三附属医院a.医院感染管理科; b.微生物实验室; c.检验科,贵州都匀 558000
Author(s):
XIE Zhao-yuna; b; HU Yangb; YANG Zhong-lingc; SUN Jinga
a.Hospital Infection Control Branch; b.Microbiology Laboratory; c.Department of Clinical Laboratory,the Third Affiliated Hospital of Guizhou Medical University,Guizhou Duyun 558000,China
关键词:
肠杆菌科细菌;  环丙沙星;  抗菌药物;  耐药性
分类号:
R378.2; R446.5
DOI:
10.3969/j.issn.1671-7414.2016.02.019
文献标志码:
A
摘要:
目的 比较临床感染肠杆菌科细菌环丙沙星耐药菌株与环丙沙星敏感菌株的耐药性差异,为临床合理选用有效抗菌药物提供参考。方法 对贵州医科大学第三附属医院2011年6月～2014年11月临床感染标本中分离出2 073株肠杆菌科细菌,分为环丙沙星耐药菌株组和环丙沙星敏感菌株组,采用VITEK-32分析鉴定系统进行病原菌鉴定,抗菌药物药敏试验及其结果判断均采用美国临床实验室标准化协会(CLSI)推荐的K-B法及其结果判断标准,统计分析应用SPSS19.0软件。结果在2 073株肠杆菌科细菌中肠杆菌科细菌环丙沙星耐药菌检出率达30.39%(630/2073); 分离出的630株耐环丙沙星肠杆菌科细菌对31种抗菌药物的药敏试验中有头孢唑林、氨苄西林/舒巴坦、头孢呋肟、妥布霉素和磺胺甲恶唑/甲氧苄啶等24种抗菌药物耐药率分别为90.00%(567/630),83.02%(523/630),93.80%(591/630),75.87%(478/630)和87.46%(551/630); 肠杆菌科细菌耐环丙沙星菌株的耐药性较环丙沙星敏感菌株严重,除亚胺培南和美罗培南外两者耐药率比较差异均具有统计学显著性意义(χ2 =11.95～1762.21,P<0.001～0.000)。结论 临床感染肠杆菌科细菌耐环丙沙星菌株检出率不断上升,耐药性明显高于环丙沙星敏感菌株,表现为同时对多种抗菌药物高耐药。
Abstract:
Objective To compare the difference of drug resistance between ciprofloxacin resistant strains and sensitive strains of ciprofloxacin in clinical infection,and provide reference for clinical rational use ofantibiotics.Methods 2 073 strains of Escherichia coli were isolated from clinical specimens from November 2014 to June 2011 in theAffiliated Hospital of Guizhou Medical University.Strains of ciprofloxacin resistant strains were divided into ciprofloxacin resistant strains and ciprofloxacin sensitive strains.VITEK-32 analysis was used to determine the pathogenic bacteria,and the results were used to determine the K-B method and the results were used to determine the standard of SPSS 19.0.Results In 2 073 strains of Enterobacteriaceae resistant to ciprofloxacin bacteria detection rate was 30.39%(630/2 073); 630 strains of ciprofloxacin resistantEnterobacteriaceae bacteria to 31 kinds of antimicrobial susceptibility testwith cefazolin,ampicillin/Shubatan,cefuroxime,tobramycin and sulfamethoxazole/trimethoprim 24 kinds of antimicrobial drug resistance rate was 90.00%(567/630),83.02%(523/630),93.80%(591/630),75.87%(478/630)and 87.46%(551/630),respectively.Drug resistance of Enterobacteriaceae in ciprofloxacin resistant strains compared with ciprofloxacin susceptible strains,except imipenemand meropenem resistance rate between the difference was statistically significant statistical difference(χ2=11.95～1762.21,P<0.001～0.00).Conclusion The detection rate of ciprofloxacin resistant strainsin clinical infection is increasing,and the drug resistance was significantlyhigher than that of ciprofloxacin resistant strains.

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备注/Memo

备注/Memo:
基金项目:贵州省黔南州社会发展科技项目(黔南科合社字[2013]20号)。 作者简介:谢朝云(1968-),男,水族,医学硕士,副主任医师,主要研究方向:医院感染防控及细菌耐药性监测研究,Tel:138854479 79,E-mail:xcu2009@163.com。
更新日期/Last Update: 2016-02-20