[1]张阳东a,陈 洁b,楚瑞雪,等.冠心病患者α-内收蛋白基因多态性与表达水平研究[J].现代检验医学杂志,2016,31(03):59-61.[doi:10.3969/j.issn.1671-7414.2016.03.016]
　ZHANG Yang-donga,CHEN Jieb,CHU Rui-xue,et al.Study on Polymorphisms and Genes Expression Levels of α-Adducin in Patients with Coronary Artery Disease[J].Journal of Modern Laboratory Medicine,2016,31(03):59-61.[doi:10.3969/j.issn.1671-7414.2016.03.016]
点击复制

冠心病患者α-内收蛋白基因多态性与表达水平研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
59-61
栏目:
论著
出版日期:
2016-06-15

文章信息/Info

Title:
Study on Polymorphisms and Genes Expression Levels of α-Adducin in Patients with Coronary Artery Disease
文章编号:
1671-7414(2016)03-059-03
作者:
张阳东1a; 陈 洁1b; 楚瑞雪2; 王利营1a; 云洁1a; 安 娟1; 方 靓1a
1.火箭军总医院 a.特勤医学科;
b.护理部,北京 100088;
2.火箭军礼士路门诊部,北京 100820
Author(s):
ZHANG Yang-dong1a; CHEN Jie1b; CHU Rui-xue2; WANG Li-ying1a; YUN Jie1a;  AN Juan1a; FANG Liang1a
1a.Special Service Medicine Department;
1b.Nursing Department,the General Hospital of the PLA Rocket Force,Beijing 100088,China;
2.Lishilu Clinic of the PLA Rocket Force,Beijing 100820,China
关键词:
α-内收蛋白;  冠心病;  单核苷酸多态性;  基因表达
分类号:
R541.4; Q786
DOI:
10.3969/j.issn.1671-7414.2016.03.016
文献标志码:
A
摘要:
目的 探讨α-内收蛋白基因(ADD1)的单核苷酸多态性(SNP)及表达水平与冠心病(CAD)的相关性。方法 提取2011年1月～2013年10月就诊于火箭军总医院的114例CAD患者和116例健康对照者外周血白细胞DNA和RNA,应用荧光标记单碱基延伸及杂交技术检测ADD1基因的rs3775067和rs1263359多态性,应用荧光标记及毛细管电泳技术检测ADD1的基因表达水平。结果 ADD1基因2个SNP的基因型频率和等位基因频率在两组间差异无统计学意义(χ2=0.018～1.317,P值均>0.05)。CAD组的ADD1基因表达水平为0.226±0.284,明显高于健康对照组(0.153±0.144),差异有统计学意义(P<0.05)。通过对不同基因型间表达水平的比较,结果显示CAD组rs3775067的TC型患者的基因表达水平(0.250±0.319)明显高于健康对照组(0.154±0.156,P<0.05),余各基因型两组间表达水平差异无统计学意义(t=0.557～1.867,P值均>0.05)。结论 ADD1基因表达水平的升高是CAD的危险因素,而rs3775067和rs1263359多态性与CAD的易感性无关。
Abstract:
Objective To investigate the association between single nucleotide polymorphisms(SNP)and expression levels of α-adducin(ADD1)gene in coronary artery disease(CAD)patients.Methods Extracted DNA and RNA samples of peripheral blood white cells from 114 CAD patients and 116 healthy individuals in Jan 2011 to Oct 2013 from the General Hospital of the PLA Rocket Force.SNPs of rs3775067 and rs1263359 mutations in the ADD1 gene were analyzed with allele-specific fluorogenic oligonucleotide probes combining hybridization.The gene expression levels were analyzed with fluorescence labeled and capillary electrophoresis technology.Results The frequencies of the genotypes and alleles of the two SNPs in the ADD1 gene were not significantly different between the two groups(χ2=0.018～1.317,all P>0.05).The ADD1 gene expression levels of CAD group(0.226±0.284)were obviously higher than that of control group(0.153±0.144,P<0.05).The gene expression levels of TC genotype of rs3775067 were obviously higher in CAD group(0.250±0.319)than that of control group(0.154±0.156,P<0.05),but the levels of the other genotypes had no significant difference between the two groups(t=0.557～1.867,all P>0.05).ConclusionThe elevated ADD1 gene expression level would be risk factor for CAD.The polymorphisms of rs3775067 and rs1263359 had no relevance with CAD susceptibility.

参考文献/References:

[1] 翟丽华,梁 旭,王沙燕.内收蛋白α亚单位遗传多态性与原发性高血压[J].中华老年医学杂志,2011,31(5):901-903. Zhai LH,Liang X,Wang SY.Genetic polymorphisms of alpha-adducin and essential hypertension[J].Chinese Journal of Geriatrics,2011,31(5):901-903.
[2] 张阳东,温新宇,董 矜,等.冠心病相关基因多重单核苷酸多态性分型检测法的构建[J].中国实验诊断学,2011,15(9):1428-1432. Zhang YD,Wen XY,Dong J,et al.Establishment of a multiplex genotyping method for single nucleotide polymorphisms of genes associated with coronary artery disease[J].Chinese Journal of Laboratory Diagnosis,2011,15(9):1428-1432.
[3] 张阳东,楚瑞雪,时 磊,等.2型糖尿病患者外周血管家基因表达水平比较[J].现代检验医学杂志,2011,26(1):9-10. Zhang YD,Chu RX,Shi L,et al.Comparison of housekeeping genes expression levels in peripheral blood of type 2 diabetes mellitus patients[J].Journal of Modern Laboratory Medicine,2011,26(1):9-10.
[4] Chen CL,Lin YP,Lai YC,et al.α-adducin translocates to the nucleus upon loss of cell-cell adhesions[J].Traffic,2011,12(10):1327-1340.
[5] 李刚强,朱 瑞,樊济海.原发性高血压患者α-内收蛋白基因单核苷酸多态性与复方缬沙坦降压治疗疗效的相关性研究[J].临床内科杂志,2012,29(4):276-278. Li GQ,Zhu R,Fan JH.Relationship between the angiotensin Ⅱ type 2receptor polymorphism and antihypertensive effect of valartan/Hydrochlorothiazide[J].Journal of Clinical Internal Medicine,2012,29(4):276-278.
[6] Li YY.α-Adducin Gly460Trp gene mutation and essential hypertension ina Chinese population:a meta-analysis including 10 960 subjects[J].PLoS One,2012,7(1):e30214.
[7] Wang LF,Zheng B,Zhao HG,et al.α-Adducin gene G614T polymorphisms inessential hypertension patients with high low density lipoprotein(LDL)levels[J].Indian J Med Res,2014,139(2):273-278.
[8] 王 丛,孙 刚,丁燕程,等.α-内收蛋白基因多态性与大动脉弹性相关性研究[J].中国分子心脏病学杂志,2007,7(4):214-217. Wang C,Sun G,Ding YC,et al.The association beween α-adducin gene polymorphism and big artery elasticity[J].Molecular Cardiology of China,2007,7(4):214-217.
[9] Yan L,Lutgarde T,Tatiana K,et al.Cardiovascular risk in relation toα-adducin Gly460Trp polymorphism and systolic pressure[J].Hypertension,2005,46(3):527-532.
[10] Wang L,Chen S,Zhao Q,et al.Association between genetic variants of the ADD1 and GNB3 genes and blood pressure response to the cold pressor test in a Chinese Han population:the GenSalt Study[J].Hypertens Res,2012,35(8):805-810.

相似文献/References:

[1]赵 佳a,左 林b,姚创利a,等.锰超氧化物歧化酶基因变异 与血脂和同型半胱氨酸水平的关系研究[J].现代检验医学杂志,2016,31(03):12.[doi:10.3969/j.issn.1671-7414.2016.03.004]
　ZHAO Jiaa,ZUO Linb,YAO Chuang-lia,et al.Study on the Relationship between Manganese Superoxide Dismutase Gene Variants and the Levels of Blood Lipid and Homocysteine[J].Journal of Modern Laboratory Medicine,2016,31(03):12.[doi:10.3969/j.issn.1671-7414.2016.03.004]
[2]程俊杰,李 静,梁西强,等.非高密度脂蛋白胆固醇 与冠心病患者心功能分级的相关性研究[J].现代检验医学杂志,2016,31(03):43.[doi:10.3969/j.issn.1671-7414.2016.03.012]
　CHENG Jun-jie,LI Jing,LIANG Xi-qiang,et al.Correlation between Non High Density Lipoprotein Cholesterol and Cardiac Function in Patients with Coronary Heart Disease[J].Journal of Modern Laboratory Medicine,2016,31(03):43.[doi:10.3969/j.issn.1671-7414.2016.03.012]
[3]刘运端,林世恒.脂联素及其基因多态性与广西瑶族冠心病的关系[J].现代检验医学杂志,2015,30(04):56.[doi:10.3969/j.issn.1671-7414.2015.04.015]
　LIU Yun-duan,LIN Shi-heng.Study on Relations between Adiponectin and Its Gene Polymorphism and Yao Coronary Heart Disease of Guangxi District[J].Journal of Modern Laboratory Medicine,2015,30(03):56.[doi:10.3969/j.issn.1671-7414.2015.04.015]
[4]姚创利a,赵 佳a,黎 阳a,等.锰超氧化物歧化酶9 Ala/Val基因多态性对冠心病的影响[J].现代检验医学杂志,2015,30(02):1.[doi:10.3969/j.issn.1671-7414.2015.02.001]
　YAO Chuang-lia,ZHAO Jiaa,LI Yanga,et al.Effects of Manganese Superoxide Dismutase 9 Ala/Val Genetic Polymorphisms on Coronary Heart Disease[J].Journal of Modern Laboratory Medicine,2015,30(03):1.[doi:10.3969/j.issn.1671-7414.2015.02.001]
[5]姚创利,赵 佳,鲁旭娟,等.冠心病患者血清脂蛋白相关磷脂酶A2水平与锰超氧化物歧化酶9 Ala/Val基因多态性关系探讨[J].现代检验医学杂志,2017,32(01):26.[doi:10.3969/j.issn.1671-7414.2017.01.008]
　YAO Chuang-li,ZHAO Ja,LU Xu-juan,et al.Study on the Relationship between Lipoprotein Associated Phospholipase A2 Level and Manganese Superoxide Dismutase 9 Ala/Val Genetic Polymorphism in Patients with Coronary Heart Disease[J].Journal of Modern Laboratory Medicine,2017,32(03):26.[doi:10.3969/j.issn.1671-7414.2017.01.008]
[6]张阳东,周建荣,楚瑞雪,等.血管紧张素Ⅱ1型受体3个单核苷酸多态性与冠心病的相关性分析[J].现代检验医学杂志,2017,32(02):57.[doi:10.3969/j.issn.1671-7414.2017.02.015]
　ZHANG Yang-dong,ZHOU Jian-rong,CHU Rui-xue,et al.Correlation between the Three Single Nucleotide Polymorphisms in Angiotensin Ⅱ Type one Receptor Gene and Coronary Artery Disease[J].Journal of Modern Laboratory Medicine,2017,32(03):57.[doi:10.3969/j.issn.1671-7414.2017.02.015]
[7]刘夏炎,余安运,于佩方,等.RDW,HCY和LP(a在高血压及冠心病中的临床应用[J].现代检验医学杂志,2017,32(04):80.[doi:10.3969/j.issn.1671-7414.2017.04.022]
　LIU Xia-yan,YU An-yun,YU Pei-fang,et al.Clinical Application of RDW,HCY and LP(a)in High Blood Pressure and Combined Coronary Heart Disease[J].Journal of Modern Laboratory Medicine,2017,32(03):80.[doi:10.3969/j.issn.1671-7414.2017.04.022]
[8]阿地拉·阿布里孜,刘 敏.miR-126在血液透析并发冠心病患者中检测的临床意义[J].现代检验医学杂志,2017,32(05):97.[doi:10.3969/j.issn.1671-7414.2017.05.017]
　ADILA·Abulizi,LIU Min.Clinical Significance of Detecting miR-126 in Hemodialysis with Coronary Artery Disease[J].Journal of Modern Laboratory Medicine,2017,32(03):97.[doi:10.3969/j.issn.1671-7414.2017.05.017]
[9]张阳东,周建荣,楚瑞雪,等.PECAM-1基因rs7503550位点多态性和表达水平与冠心病及2型糖尿病的相关性研究[J].现代检验医学杂志,2019,34(03):21.[doi:10.3969/j.issn.1671-7414.2019.03.005]
　ZHANG Yang-dong,ZHOU Jian-rong,CHU Rui-xue,et al.Relativity Study on rs7503550 Polymorphism and Genes Expression Levelsof PECAM-1 in Coronary Artery Disease and Type 2 Diabetes Mellitus[J].Journal of Modern Laboratory Medicine,2019,34(03):21.[doi:10.3969/j.issn.1671-7414.2019.03.005]
[10]朱志远,王海燕,吴 波,等.冠心病患者血小板和淋巴细胞比值与冠脉斑块稳定性及预后的相关性[J].现代检验医学杂志,2019,34(04):112.[doi:10.3969/j.issn.1671-7414.2019.04.027]
　ZHU Zhi-yuan,WANG Hai-yan,WU Bo,et al.Correlation between Ratio of Platelet to Lymphocyte with the Stability of Coronary Artery Plaque and Prognosis in Patients with Coronary Heart Disease[J].Journal of Modern Laboratory Medicine,2019,34(03):112.[doi:10.3969/j.issn.1671-7414.2019.04.027]

备注/Memo

备注/Memo:
作者简介:张阳东(1970-),男,医学博士,主任技师,研究方向:临床生物化学与分子生物学诊断,Tel:13671041699,E-mail: zhangyd262@sohu.com。
更新日期/Last Update: 2016-06-25