[1]吴景华,郭佳培,王志刚,等.TLR4与MyD88蛋白在慢性肾功能不全组织中的表达研究[J].现代检验医学杂志,2016,31(03):62-64.[doi:10.3969/j.issn.1671-7414.2016.03.017]
　WU Jing-hua,GUO Jia-pei,WANG Zhi-gang,et al.Study on Effect of TLR4/MyD88 Protein on Chronic Renal Failure[J].Journal of Modern Laboratory Medicine,2016,31(03):62-64.[doi:10.3969/j.issn.1671-7414.2016.03.017]
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TLR4与MyD88蛋白在慢性肾功能不全组织中的表达研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年03期
页码:
62-64
栏目:
论著
出版日期:
2016-06-15

文章信息/Info

Title:
Study on Effect of TLR4/MyD88 Protein on Chronic Renal Failure
文章编号:
1671-7414(2016)03-062-04
作者:
吴景华1; 郭佳培1; 王志刚2; 王海欣3
1.华北理工大学附属医院检验科,河北唐山 063000);
2.唐山丰润区第二人民医院检验科,河北唐山 063000;
3.唐山市中医医院口腔科,河北唐山 063000
Author(s):
WU Jing-hua1; GUO Jia-pei1; WANG Zhi-gang2; WANG Hai-xin3
1.Department of Clinical Laboratory,the Affiliated Hospitalof North China University of Sicience and Technology,Hebei Tangshan 063000,China;
2.Department of Clinical Laboratory,the Second People's Hospital in Fengrun District, Hebei Tangshan 063000,
关键词:
慢性肾功能不全;  Toll样受体4/髓样细胞分化因子88蛋白;  肌酐;  尿素氮
分类号:
R692; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.03.017
文献标志码:
A
摘要:
目的 通过研究Toll受体4(TLR4)及髓样细胞分化因子88(MyD88)在慢性肾功能不全组织中的表达,探讨TLR4/MyD88蛋白在慢性肾功能不全发生发展中的作用。方法 选取2011.07～2014.05华北理工大学附属医院确诊的40例肾功能不全的患者为观察对象,40例肾癌切除患者未被浸润的肾组织为对照,通过免疫组化方法检测慢性肾功能不全患者肾脏组织中TLR4及MyD88的表达情况,通过腺嘌呤(200mg/kg)连续灌胃制备大鼠模型,定时逆转录PCR(RT-PCR)及Western blot检测TLR4及MyD88的表达,同时将大鼠模型分为TLR4组、未阻断组和IgG对照组分别检测外周血中尿素氮(BNN)及肌酐(RE)的浓度。结果 慢性肾功能不全患者肾脏组织中TLR4及MyD88的表达明显高于正常对照组; 腺嘌呤制备的大鼠模型中的TLR4及MyD88的基因及蛋白的表达明显高于正常大鼠; TLR4阻断组尿素氮(15.65±3.97mmol/L)明显低于未阻断组(23.33±7.62mmol/L)及IgG对照组(26.33±6.77mmol/L)(t=2.887,P=0.045); 肌酐(523.89±52.67μmol/L)也明显低于未阻断组(789.51±98.17μmol/L)及IgG对照组(809.51±94.19μmol/L)(t=4.125,P=0.015)。结论 TLR4及MyD88蛋白在慢性肾功能不全中表达增加,并且显著促进疾病的发展过程。
Abstract:
Objective To explore the expression of TLR4 andMyD88 in chronic renal failure and its role in the development of chronic renalfailure.Methods 40 cases of patients diagnosed chronic renal failure during 2011.07～2014.05 were selected as observation subjects,and renal tissues without invasion of 40 cases patients with renal carcinomaresection were chosen as control.The expression of TLR4 and MyD88 in chronic renal failure was detected by IMH.The mice model was stabled establish through gavage of adenine(200 mg/kg).And the TLR4 and MyD88 expression was detected byRT-PCR and Western blot.The models was divided into three groups:TLR4 blocking group,TLR4 non-blocking and control group.And the BUN and CRE were detectedby biochemical analyzer in three groups.Results The expression of TLR4 and MyD88 were higher in chronic renal failure than in normals.The TLR4 and MyD88 were also higher in chronic renal failure model mice.Thelevels of BUN(15.65±3.97 mmol/L)in TLR4 blocking group were lower than which in TLR4 non-blocking group(23.33±7.62 mmol/L)and which in IgG group(26.33±6.77mmol/L)(t=2.887,P=0.045).The CRE levels were the lowest in TLR4 -blocking group(523.89±52.67 μmol/L)compared with the TLR4 non-blocking group(789.51± 98.17 μmol/L)and the IgG group(809.51±94.19 μmol/L)(t=4.125,P=0.015).Conclusion The increased expression of TLR4 and MyD88 in chronic renal failure significantly would promote the development of chronic renal failure.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:吴景华(1976-),男,医学博士,副教授,专业:分子免疫学,E-mail:tswujing hua@163.com。
更新日期/Last Update: 2016-06-25