[1]马巍娜,刘雪林,宋宏彬,等.急性淋巴细胞白血病单链抗体(scFv)的筛选与鉴定[J].现代检验医学杂志,2016,31(05):46-49.[doi:10.3969/j.issn.1671-7414.2016.05.012]
　MA Wei-na,LIU Xue-lin,SONG Hong-bin,et al.Screening and Characterization of Human Phage Antibody to Permethrin[J].Journal of Modern Laboratory Medicine,2016,31(05):46-49.[doi:10.3969/j.issn.1671-7414.2016.05.012]
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急性淋巴细胞白血病单链抗体(scFv)的筛选与鉴定()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年05期
页码:
46-49
栏目:
论著
出版日期:
2016-10-21

文章信息/Info

Title:
Screening and Characterization of Human Phage Antibody to Permethrin
文章编号:
1671-7414(2016)05-046-04
作者:
马巍娜1; 刘雪林2; 宋宏彬2; 沈建良1; 黄友章1; 刘 毅1; 向 丹1
1.中国人民解放军海军总医院血液科,北京 100037;
2.中国人民解放军军事医学科学院十所,北京 100039
Author(s):
MA Wei-na1; LIU Xue-lin2; SONG Hong-bin2; SHENJian-liang1; HUANG You-zhang1; LIU Yi1;  XIANG Dan1
1.Department of Hematology,General Hospital of PLA Navy,Beijing 100037,China;
2.PLA Disease Control and Prevention,Beijing 100039,China
关键词:
抗体;  噬菌体表面展示技术;  急淋白血病;  scFv
分类号:
R557.4; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.05.012
文献标志码:
A
摘要:
目的 筛选急性淋巴细胞白血病病人单链可变区scFv抗体,为进一步表达并得到其特异性强的抗体片段创造条件。方法 实验利用初诊急性淋巴细胞性白血病病人血清为包被抗原,采用噬菌体表面展示技术,从半合成的人源噬菌体抗体库中筛选其特异性噬菌体抗体,首先把靶抗原包被于免疫平板后,加入噬菌体抗体库,这样能与靶抗原特异性结合的噬菌抗体就被固定在免疫平板上,不能特异结合的噬菌体则被漂洗掉; 将特异结合的噬菌体洗脱下来,侵染大肠埃希菌,就可以得到含特异抗体基因的噬菌粒。结果 经过3轮“吸附-洗脱-扩增”筛选过程,获得抗原特异性较强的白血病病人可变区噬菌体抗体片段并鉴定。结论 获得了一株亲和性较好的抗体片段,为下一步片段表达、鉴定及临床应用研究创造了条件。
Abstract:
Objective To do screening acute lymphoblastic leukemia patients scFv antibody single chain variable region to create conditionsfor the expression and obtain further specificity of antibody fragments.Methods In this study,patients with newly diagnosed acute lymphoblastic leukemia serum as coating antigen using phage display technology,screening phage antibody specificity from the semi-synthetic human phage antibodylibraries,the first to target the immune antigen-coated tablet,phage librarywas added,so that with the target antigen-specific binding phage antibody wasimmobilized on plates immunization,could not be specifically bound phages wererinsed.The eluted specific binding phage,E.coli infection.Could get thespecific antibody gene containing phagemid.Results Afterthree “adsorption-elution-amplification” screening process,got stronger leukemia patient antigen-specific phage antibody variable region fragment and identification.Conclusion Got better strain affinity antibody fragments,to create the conditions for the next fragment expression,identification and clinical application.

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备注/Memo

备注/Memo:

作者简介:马巍娜(1983-),女,硕士研究生,研究方向:微生物控制技术,Tel:010-66958485,E-mail:mv4477@sina.com。
更新日期/Last Update: 2016-10-30