[1]丁磊,刘鹏,叶辛,等.miR-491-5p在原发免疫性血小板减少症患者中的表达及临床意义[J].现代检验医学杂志,2016,31(06):17-19.[doi:10.3969/j.issn.1671-7414.2016.06.005]
　DING Lei,LIU Peng,YE Xin,et al.Expression and Clinical Significance of miR-491-5p in Patients with Primary Immune Tombocytopenia[J].Journal of Modern Laboratory Medicine,2016,31(06):17-19.[doi:10.3969/j.issn.1671-7414.2016.06.005]
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miR-491-5p在原发免疫性血小板减少症患者中的表达及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年06期
页码:
17-19
栏目:
论著
出版日期:
2016-12-20

文章信息/Info

Title:
Expression and Clinical Significance of miR-491-5p in Patients with Primary Immune Tombocytopenia
作者:
丁磊1; 刘鹏2; 叶辛2; 邓安梅2; 任永亚3; 吴天勤3; 钱琤3
丁 磊1,刘 鹏2,叶 辛2,邓安梅2,任永亚3,吴天勤3,钱 琤3
Author(s):
DING Lei1; LIU Peng2; YE Xin2; DENG An-mei2; REN Yong-ya3; WU Tian-qin3; QIAN Cheng3
1.Wuzhong District Hospital of Integrated Chinese and WesternMedicine,Jiangsu Suzhou 215200,China; 2.Changhai Hospital,the Second Militray Medical University, Shanghai 200433,China; 3.100th Hospital of PLA,Jiangsu Suzhou 215007,China
关键词:
原发免疫性血小板减少症;  miR-491-5P;  血小板计数;  外周血单个核细胞
分类号:
R558.2; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.06.005
文献标志码:
A
摘要:
目的 研究miR-491-5p在原发免疫性血小板减少症(primary immune thrombocytopenia,ITP)初发患者外周血单个核细胞(peripheral blood mononuclear cells,PBMC)中的表达水平及临床意义。方法 选取2014年9月～2015年9月解放军第100医院血液科住院的初诊ITP患者40例(初诊组),经治疗后完全缓解者22例(完全缓解组),40例健康体检者为对照组。采集外周静脉血2 ml,分离PBMC,提取细胞总RNA,逆转录合成cDNA,以U6为内参,应用实时荧光定量PCR(real time PCR)的方法检测miR-491-5p的表达量; 分析miR-491-5p与血小板计数之间的相关性。结果 初诊组(5.07±1.70)与对照组(1.92±0.95)外周血单个核细胞miR-491-5p的表达相比上调,差异有统计学意义(t=9.36,P<0.01); 治疗后完全缓解组(2.32±1.07 )与其初诊时(4.95±0.83)外周血单个核细胞miR-491-5p的表达相比下调,差异有统计学意义(t=8.94,P<0.01); ITP患者外周血单个核细胞中miR-491-5p的表达量与血小板计数呈负相关(r=-0.769 7,P<0.05)。结论 miR-491-5p在ITP初发患者外周血单个核细胞中高表达,表明其可能与ITP的发病相关,或成为未来ITP诊疗潜在的标志物。
Abstract:
Objective To explore the expression and clinical significance of microRNA(miR-491-5p)in the peripheral blood mononuclear cells(PBMCs)of primary Immune Thrombocytopenia(ITP)patients.Methods40 ITP patients(study group)and 40 healthy subjects(control group)were collected from the Department of Hematology,100th Hospital of PLA Hospital.After treatment,22 cases of complete remission as a complete remission.2ml peripheral blood taken from them,PBMCs were separated and total RNA was extracted from the cells,cDNA was synthesized by reverse transcription,U6 for reference,then determined the expression of miR-491-5p with the real-time fluorescent quantitative PCR(real time PCR); To analyze the correlation between miR-491-5P and platelet count.Results Compared newly diagnosed group(5.07±1.70)with control group(1.92±0.95)expression in peripheral blood mononuclear cells up-regulated miR-491-5P compared,the difference was statistically significant(t=9.36,P<0.01).Compared complete remissiongroup(2.32±1.07)with their first visit(4.95±0.83)the expression of miR-491-5P in peripheral blood mononuclear cells compared to cut,the differencewas statistically significant(t=8.94,P<0.01).The miR-491-5P level in ITP patients was negativel correlated with the platelet counts(r=-0.769 7,P<0.05).Conclusion The miR-491-5P expression inITP patients was significantly increased,which suggests that it may be relatedto the pathogenesis of ITP,or to be a potential marker for the diagnosis and treatment of ITP in the future.

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备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81471605,81401358,81501397,31500721,81501398,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海科学教育基金(D14017),军队科研基金(BWS14J023,12MA056,15ZD009),美捷登基金(MJR20150019)。 作者简介:丁 磊(1988-),男,本科,检验技师,主要从事临床免疫学及免疫学检验研究,E-mail:dinglei_0126@126.com。 通讯作者:钱 琤,主治医师,E-mail:qiancheng824@163.com。 吴天勤,主任医师,E-mail:wtq100@vip.163.com,共同通讯作者。
更新日期/Last Update: 2016-12-20