[1]陆丹倩,芮勇宇,杨秋,等.耐碳青霉烯类鲍曼不动杆菌基因检测及同源性分析[J].现代检验医学杂志,2016,31(06):62-65.[doi:10.3969/j.issn.1671-7414.2016.06.017]
　LU Dan-qian,RUI Yong-yu,YANG Qiu,et al.Gene Detection and Homology Analysis of Acinetobacter-Resistant Acinetobacter Baumannii[J].Journal of Modern Laboratory Medicine,2016,31(06):62-65.[doi:10.3969/j.issn.1671-7414.2016.06.017]
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耐碳青霉烯类鲍曼不动杆菌基因检测及同源性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年06期
页码:
62-65
栏目:
论著
出版日期:
2016-12-20

文章信息/Info

Title:
Gene Detection and Homology Analysis of Acinetobacter-Resistant Acinetobacter Baumannii
作者:
陆丹倩1; 芮勇宇2; 杨秋2; 顾向明1; 邓冲1; 王巧媚1; 邓聚辉1
1.广东省中山市中医院检验科,广东中山 528400; 2.南方医科大学南方医院,广州 510515
Author(s):
LU Dan-qian1; RUI Yong-yu2; YANG Qiu2; GU Xiang-ming1; DENG Chong1;  WANG Qiao-mei1; DENG Ju-hui1
1.Department of Clinical Laboratory, Guangdong Zhongshan Hospital of Tranditional Chinese Medicine,Guangdong Zhongshan 528400,China; 2.Nanfang Hospital,Southern Medical University,Guangzhou510515,China
关键词:
耐碳青霉烯类鲍曼不动杆菌;  碳青霉烯酶;  PCR;  ERIC-PCR
分类号:
R378; Q781
DOI:
10.3969/j.issn.1671-7414.2016.06.017
文献标志码:
A
摘要:
目的 临床分离出的耐碳青霉烯类鲍曼不动杆菌(CRAB)耐药基因检测及同源性分析。方法 收集中山市中医院2012年7月～2013年12月临床分离40株CRAB,用PCR法对碳青霉烯类基因进行扩增、序列分析; 应用ERIC-PCR研究其传播机制。结果 40株CRAB所有菌株且被检测到含有OXA-51-like基因为40株(100%),OXA-23-like基因为38株(95%),未检出NDM,VIM,IPM,OXA-24-like及OXA-58-like基因; ERIC-PCR聚类分析相似大于0.8,可认为是同一克隆群; 这40株菌在相似水平被聚为33个类群(克隆株)。结论 临床分离的CRAB主要携带碳青霉烯酶基因型为OXA-23-like,OXA-51-like和ERIC-PCR是进行鲍曼不动杆菌同源性分析的有效方法。
Abstract:
Objective To detect and have homology analysisof resistant genes in clinical isolates of resistant Acinetobacter Bauman(CRAB).Methods Collected 40 clinical isolates of CRAB in Zhongshan Hospital of Traditional Chinese Medicine from July 2012 to December 2013,using PCR method for the amplification of the gene of carbon,sequence analysis.Application of ERIC-PCR to study the transmission mechanism.Results 40 strains of crab all isolates and detected containing oxa-51-like gene for 40 strains(100%),the gene oxa-23-like geneand for 38 strains(95%),were not detected in NDM,VIM,IPM,gene OXA-24-like and OXA-58-like.ERIC-PCR clustering analysis of similarity was greater than 0.8,that was the same colonies.The 40 strains of bacteria at a similar level were clustered into 33 taxa(clone).Conclusion The clinical isolates of CRAB mainly carry on the effective method of OXA-23-like,OXA-51-like,and ERIC-PCR for the homology analysis of Acinetobacter Bauman.

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备注/Memo

备注/Memo:
基金项目:中山市科技局基金项目(2014A1FC052)。 作者简介:陆丹倩(1972-),女,学士,主任技师,从事临床微生物学研究,Tel:0760-89980781,E-mail:ludanqian@163.com。
更新日期/Last Update: 2016-12-20