[1]李腾达,陈 燕,龙曙萍,等.原发性胆汁性肝硬化患者外周血单个核细胞中IL-37表达增高及临床意义[J].现代检验医学杂志,2017,32(01):12-15.[doi:10.3969/j.issn.1671-7414.2017.01.004]
　LI Teng-da,CHEN Yan,LONG Shu-ping,et al.Increasing Expression of IL-37 in Peripheral Blood Mononuclear Cell of Patients with Primary Biliary Cirrhosis and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2017,32(01):12-15.[doi:10.3969/j.issn.1671-7414.2017.01.004]
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原发性胆汁性肝硬化患者外周血单个核细胞中IL-37表达增高及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年01期
页码:
12-15
栏目:
论著
出版日期:
2017-01-25

文章信息/Info

Title:
Increasing Expression of IL-37 in Peripheral Blood Mononuclear Cell of Patients with Primary Biliary Cirrhosis and Its Clinical Significance
文章编号:
1671-7414(2017)01-012-04
作者:
李腾达1; 陈 燕1; 龙曙萍1; 谷明莉1; 刘 鹏1; 吴林洪1; 邓顺江1; 张薇薇1; 钱 铮2; 邓安梅1
1.第二军医大学附属长海医院,上海 200433;
2.解放军100医院,江苏苏州 215007
Author(s):
LI Teng-da1; CHEN Yan1; LONG Shu-ping1; GU Ming-li1; LIU Peng1;  WU Lin-hong1; DENG Shun-jiang1; ZHANG Wei-wei1; QIAN Cheng2; DENG An-mei1
1.Changhai Hospital Affiliated to Second Military Medical University, Shanghai 200433,China;
2.No.100 Hospital of PLA,Jiangsu Suzhou 215007,China
关键词:
原发性胆汁性肝硬化;  白细胞介素-37;  白细胞介素-17;  白细胞介素-6;  表皮生长因子-β
分类号:
R575.22; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.01.004
文献标志码:
A
摘要:
目的 检测原发性胆汁性肝硬化(PBC)患者外周血单个核细胞(PBMCs)中IL-37的表达水平,进一步探究其在PBC致病过程中的临床意义。方法 收集2013年6月～2015年8月于长海医院确诊的42例PBC患者和38例同期体检的健康对照者外周血,采用蔗糖密度梯度离心法分离PBMCs,以实时荧光定量聚合酶链反应(qRT-PCR)检测PBMCs中IL-37的mRNA表达水平,以酶联免疫吸附法(ELISA)检测血浆中IL-37,IL-6,IL-17,TNF-α,TGF-β,IL-18和IL-23的蛋白水平,同时记录PBC患者的病理性分期。对IL-37与IL-6,TNF-α,IL-17,TGF-β,IL-18,IL-23进行Pearson相关性分析,对IL-37与PBC疾病分期进行Spearman秩相关性分析。结果 实验组与对照组IL-37的mRNA水平和蛋白水平分别为2.81±0.94 vs 1.09±0.56,356.14±169.36 pg/ml vs 86.68±48.23 pg/ml,差异具有统计学意义(t=9.811,9.462,P均＜0.000 1)。相关性分析表明:IL-37与IL-17,TNF-α,IL-6,TGF-β呈正相关(r=0.561 2,0.661 9,0.672 1,0.765 3; P值均＜0.001),与疾病分期(Ⅰ～Ⅳ)呈正相关(rs=0.348 9,P＜0.05)。结论 IL-37可能参与了PBC的致病过程,对疾病预测与诊断有重要意义。
Abstract:
Objective To test the expression level of IL-37 in peripheral blood mononuclear cells(PBMCs)of patients with primary biliarycirrhosis(PBC)and further explore its clinical significance in the pathological process of PBC.Methods Peripheral blood samples werecollected from 42 patients diagnosed as PBC and 38 health individuals examined at the same time during June 2013 to August 2015 in Changhai Hospital.PBMCs were separated by sucrose density gradient centrifugation,qualified Real Time-Polymerase Chain Reaction(qRT-PCR)was used to measure IL-37 mRNA expression level in PBMCs.Enzyme-Linked Immuno Sorbent Assay(ELISA)was to measure the protein level of IL-37,IL-6,IL-17,TNF-α,TGF-β,IL-18 and IL-23 in plasma.Meanwhile,the pathological stages of PBC cases were recorded.Pearson correlation analysis was performed on IL-37 and IL-6,TNF-α,IL-17,TGF-β,IL-18 and IL-23.Spearman rank correlation analysis was on IL-37 and pathological stages of PBC.Results The mRNA and protein level of IL-37 in experimental and controlled group were 2.81±0.94 vs 1.09±0.56,356.14±169.36 pg/ml vs 86.68±48.23 pg/ml separately(t=9.811,9.462,P＜0.000 1),with statistical differences.The correlation analysis showed thatIL-37 was positively related with IL-17,TNF-α,IL-6 and TGF-β(r=0.561 2,0.661 9,0.672 1,0.765 3,P＜0.001),and disease stages(Ⅰ～Ⅳ)(rs=0.348 9,P＜0.05).Conclusion IL-37 might involve inthe pathological process of PBC,and it is significant for disease prediction anddiagnosis.

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备注/Memo

备注/Memo:
基金项目:973计划(2013CB531606),国家自然科学基金(81471605,81401358,81501397,31500721,81501398,81302579,81273282,81202353),上海申康基金(SHDC22014014),上海教育科学基金(D14017),军队科研基金(BWS14J023,12MA056,15ZD009,15XD007),美捷登基金(MJR20150019)。
作者简介:李腾达(1990-),女,在读硕士研究生,主要从事自身免疫研究,Tel:18302192042,E-mail:tengdali@smmu.edu.cn。
陈 燕(1977-),女,检验医师,主要从事感染免疫研究,E-mail:chenyan770628@163.com,共同第一作者。
通讯作者:邓安梅,女,教授,E-mail:amdeng70@163.com。
钱 铮,女,主治医师,E-mail:qiancheng824@126.com,共同通讯作者。
更新日期/Last Update: 2017-01-20