[1]朱影明,许新强,周 强,等.广东地区2型糖尿病与抵抗素基因位点rs2161490和rs1423096多态性的相关性研究[J].现代检验医学杂志,2017,32(01):48-52.[doi:10.3969/j.issn.1671-7414.2017.01.014]
　ZHU Ying-ming,XU Xin-qiang,ZHOU Qiang,et al.Correlation Study between Resistin rs2161490 and rs1423096 and Type 2 Diabetes Mellitus[J].Journal of Modern Laboratory Medicine,2017,32(01):48-52.[doi:10.3969/j.issn.1671-7414.2017.01.014]
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广东地区2型糖尿病与抵抗素基因位点rs2161490和rs1423096多态性的相关性研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年01期
页码:
48-52
栏目:
论著
出版日期:
2017-01-25

文章信息/Info

Title:
Correlation Study between Resistin rs2161490 and rs1423096 and Type 2 Diabetes Mellitus
文章编号:
1671-7414(2017)01-048-05
作者:
朱影明,许新强,周 强,陈 波,冀天星
广州医科大学附属第二医院检验科,广州 510260
Author(s):
ZHU Ying-ming,XU Xin-qiang,ZHOU Qiang,CHEN Bo,JITian-xing
Department of Clinical Laboratory, the Second Affiliated Hospital of Guangzhou Medical University,Guangzhou510260,China
关键词:
2型糖尿病;  抵抗素;  单核苷酸多态性
分类号:
R587.1; Q786
DOI:
10.3969/j.issn.1671-7414.2017.01.014
文献标志码:
A
摘要:
目的 探讨抵抗素基因(RETN)的单核苷酸多态性(SNPs)位点rs2161490和rs1423096基因型与广东地区2型糖尿病(T2DM)的相关性。方法 收集2015年1～11月广州医科大学附属第二医院收治广东地区的新诊断为2型糖尿病患者178例为病例组和体检中心患者192例为对照组,先分析两组的基因分布频率是否达到遗传平衡具有群体代表性,比较病例组与对照组抵抗素rs2161490和rs1423096两个基因位点出现的频率是否具有统计学意义,然后比较病例组和对照组血清抵抗素rs2161490 T→C基因型和rs1423096A→G基因型的分布频率,logistic回归分析:分析抵抗素rs2161490和rs1423096两个位点各个基因型对T2DM的危险度以及调整性别以及年龄两个变量后危险度的变化; 比较病例组与对照组血脂生化指标; 对病例组rs2161490和rs1423096位点各个基因型对应的血脂生化指标TG,CHOL,HDL-C和LDL-C的水平进行相关性分析。结果 样本符合哈代-温伯格遗传定律,具有群体代表性,比较病例组与对照组抵抗素rs2161490和rs1423096两个基因位点频率:rs2161490的基因型(TT,CT,CC)比较,差异无统计学意义(P=0.834,＞0.05); rs1423096的基因型(AA,AG,GG),相比较,差异无统计学意义(P为0.960,＞0.05); 各基因型与T2DM危险度分析,差异无统计学意义(P＞0.05),调整性别以及年龄两个变量后危险度变化,差异无统计学意义(P＞0.05); TG,CHOL,HDL-C和LDL-C在各基因型表达浓度水平相关性分析,差异无统计学意义(P＞0.05)。结论 分析结果显示两位点各基因型在病例组与对照组的出现频率无统计学意义(均为P＞0.05),rs2161490与rs1423096两位点各基因型与T2DM的危险度,调整前与协变量调整后均无统计学意义(均为P＞0.05),rs2161490与rs1423096两位点各基因型与血脂水平分析差异同样均无统计学意义(均为P＞0.05),从而推断两个基因型不是广东地区T2DM的患病风险基因。
Abstract:
Objective To explore the correlation between resistins rs2161490and rs1423096 genotype with type 2 diabetes mellitus(T2DM)inGuangdong.Methods Collected 178 blood of newly diagnosed T2DM in the Second Affiliated Hospital of Guangzhou Medical University from January 2015 to November 2015 as the patient group and 192 blood of healthy physical examination as the control group.Analysis of the two groups of gene distribution frequency was to reach the genetic equilibrium,comparative two gene loci frequencies of resistin rs2161490 and rs1423096 in case group and control group wasstatistically significant,and compared the distribution frequency of rs2161490locus T→C and rs1423096 locus A→G between the patient group and the control group.Then made a logistic regression analysis:analysing the risk two loci eachgenotype of resistin rs2161490 and rs1423096 to T2DM,adjust of the gender andage,and the changes of the risk of the two variables.Comparative blood lipidsbiochemical indexes between case group and the control group,mode the correlation analysis between TG,CHOL,HDL-C and LDL-C levels of serum lipids in patients with rs2161490 and rs1423096 each genotypewere performed.Results The sample was consistent with Weinberg Hardy's law of inheritance,which was representative of the population,comparing two gene loci frequency of resistin rs2161490 and rs1423096 of case group and control group:comparinngCT,TT,CC of rs2161490 genotype,there was no statistically significant difference(P=0.834,＞0.05),and comparinng AA,AG,GG of rs2161490 genotype,there was no statistically significant difference(P=0.960,＞0.05).Eachgenotypes with T2DM risk analysis,there was no statistically significant difference(P＞0.05).Adjusting the risk change after the two variables,gender and age,there was no statistically significant difference(P＞0.05); TG,CHOL,HDL-C and LDL-C in each of the genes expression levels correlation analysis,there was no statistically significant difference(P＞0.05).Conclusion Analysis results showed that the frequency of two loci all genotypes in the case group and control group were no statistical significance(P＞0.05).The risk of two loci gene type of rs2161490 with rs1423096 and type 2 diabetes were before and after the covariate adjustment had no statistical significance(P＞0.05).Each genotype of rs2161490 with rs1423096 and lipid levels had no statistical significance(P＞0.05).Thus infer that two genotypes is not risk for type 2 diabetes genes in guangdong area.

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备注/Memo

备注/Memo:
基金项目:广东省医学科研基金项目资助(B2015040)。
作者简介:朱影明(1980-),男,本科,主管技师,广州医科大学在职研究生,研究方向:抵抗素基因的单核苷酸多态性与2型糖尿病的相关性,E-mail:43426054@qq.com。
通讯作者:周 强,主任技师,硕士生导师,E-mail:qiangzhou70@163.com。
更新日期/Last Update: 2017-01-20