[1]谈 昀a,刘 萍,白永梅b.2011～2015年鲍曼不动杆菌耐药相关基因及耐药性变迁研究[J].现代检验医学杂志,2017,32(01):53-56,60.[doi:10.3969/j.issn.1671-7414.2017.01.015]
　TAN Yuna,LIU Ping,BAI Yong-meib.Study on the Drug Resistance Related Genes and Drug Resistance of Acinetobacter Baumannii from 2011 to 2015[J].Journal of Modern Laboratory Medicine,2017,32(01):53-56,60.[doi:10.3969/j.issn.1671-7414.2017.01.015]
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2011～2015年鲍曼不动杆菌耐药相关基因及耐药性变迁研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年01期
页码:
53-56,60
栏目:
论著
出版日期:
2017-01-25

文章信息/Info

Title:
Study on the Drug Resistance Related Genes and Drug Resistance of Acinetobacter Baumannii from 2011 to 2015
文章编号:
1671-7414(2017)01-053-05
作者:
谈 昀1a; 刘 萍2; 白永梅1b
1.武警陕西省总队医院a.检验科;
b.血透中心,西安710054;
2.武警四川总队成都医院检验科,成都 610041
Author(s):
TAN Yun1a; LIU Ping2; BAI Yong-mei1b
1a.Department of Clinical Laboratory;
1b.Hemodialysis Center,Shaanxi Corps Hospital of Chinese Peoples's Armed Police Forces,Xi'an 710054,China;
2.Department of Clinical Laboratory,Chengdu Hospital of Sichuan Armed Police Corps,Chengdu 610041,Chi
关键词:
鲍曼不动杆菌;  药敏试验;  耐药基因;  耐药性
分类号:
R378; R446.5
DOI:
10.3969/j.issn.1671-7414.2017.01.015
文献标志码:
A
摘要:
目的 了解医院鲍曼不动杆菌耐药相关基因及耐药性变迁,为临床合理使用抗菌药物提供依据。方法 回顾性分析武警陕西省总队医院2011～2015年从临床送检11 521份标本中分离、培养出1 861株鲍曼不动杆菌,药敏试验采用K-B法,抗菌药物最低抑菌浓度(MIC)根据美国2014版CLSI判定标准进行判定。结果 1 861株鲍曼不动杆菌均能检测到耐药性基因gyrA,parC,OXA-51,其中对OXA-23,intⅠ1,TEM,aac(6')-Ⅰ,aac(3')-Ⅰ,ant(3”)-Ⅰ,ant(2”)-Ⅰ和Caro基因的检出率分别为50.4%,72.1%,70.9%,55.7%,56.2%,65.6%,12.9%和89.9%,而SHV,IMP,VIM等基因未检出。测序结果显示gyrA和parC基因有突变是导致喹诺酮类耐药的主要原因。鲍曼不动杆菌对常见抗生素耐药性是逐年递增趋势,且1 861株鲍曼不动杆菌有1 419株表现为多重耐药,所有菌均对多黏菌素敏感,2015年氨基糖苷类、喹诺酮类耐药率均为65%以上; 对亚胺培南、美罗培南和头孢哌酮/舒巴坦耐药率分别为35.17%,36.01%和42.40%。结论 鲍曼不动杆菌的检出率逐年上升,耐药性及多重耐药性也逐年增强,必须加强临床合理用药,控制医院感染。
Abstract:
Objective To investigate the changesof drug resistance related genes and drug resistance of Acinetobacter Baumannii in hospital,and provide evidence for rational use of antibiotics in clinic.Methods A retrospective analysis of Shaanxi Corps Hospital of Chinese Peoples's Armed Police Forces from 2011 to 2015 from clinicalsamples of 11 521 specimens were isolated and cultured 1 861 strains of Acinetobacter Baumannii,drug susceptibility test by K-B method.The antimicrobialminimum inhibitory concentration(MIC)according to the 2014 edition of the CLSI judgment criteria of judgment.Results 1 861 strains ofAcinetobacter Baumannii were detected drug resistance genes gyrA,parC,OXA-51,OXA-23,int on the 1,I TEM,AAC(6')-1,AAC(3')-1,ant(3)-1,ant(2)-1 and Caro gene detection rates were 50.4%,72.1%,70.9%,55.7%,56.2%,65.6%,12.9% and 89.9% respectively,and SHV,IMP and VIM genes were not detected.The sequencing results showed that gyrA and parC gene mutation was the main cause of quinolone resistance.The antibiotic resistance of Acinetobacter Baumannii was an increasing trend year by year,and 1 861 strains has 1 419 strains showed multiple drug resistance,all strains were sensitive to polymyxin.In 2015,quinolones aminoglycosides resistance rate was more than 65%.To imipenem,meropenem andCefoperazone/sulbactam were 35.17%,36.01% and 42.40%.ConclusionThe detection rate of Acinetobacter Baumannii is increasing year by year,and the drug resistance and multi drug resistance is increasing yearby year.It is necessary to strengthen the clinical rational drug use andcontrolthe hospital infection.

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备注/Memo

备注/Memo:
基金项目:武警部队后勤部科研基金项目(WJWSB2012-13)。
作者简介:谈 昀(1972-),女,大学本科,学士学位,主要从事微生物及免疫研究,E-mail:chhhty@sina.com。
更新日期/Last Update: 2017-01-20