[1]贾亚旭,王 成,张春妮.血清miR-193a-3p,miR-337-5p和miR-483-5p在食管鳞状细胞癌诊断和预后中的应用[J].现代检验医学杂志,2017,32(02):5-9.[doi:10.3969/j.issn.1671-7414.2017.02.002]
　JIA Ya-xu,WANG Cheng,ZHANG Chun-ni.Application of Serum miR-193a-3p,miR-337-5p and miR-483-5p in the Diagnosis and Prognosis of Esophageal Squamous Cell Carcinoma[J].Journal of Modern Laboratory Medicine,2017,32(02):5-9.[doi:10.3969/j.issn.1671-7414.2017.02.002]
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血清miR-193a-3p,miR-337-5p和miR-483-5p在食管鳞状细胞癌诊断和预后中的应用()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年02期
页码:
5-9
栏目:
论著
出版日期:
2017-03-25

文章信息/Info

Title:
Application of Serum miR-193a-3p,miR-337-5p and miR-483-5p in the Diagnosis and Prognosis of Esophageal Squamous Cell Carcinoma
文章编号:
1671-7414(2017)02-005-05
作者:
贾亚旭; 王 成; 张春妮
南京大学医学院附属金陵医院 /南京军区南京总医院 解放军临床检验医学研究所,南京 210002
Author(s):
JIA Ya-xu; WANG Cheng; ZHANG Chun-ni
PLA Institute of Clinical Laboratory Medicine, Jinling Hospital,Medical School of Nanjing University/Nanjing General Hospital of Nanjing Military Region,PLA,Nanjing 210002,China
关键词:
食管鳞状细胞癌;  miR-193a-3p;  miR-337-5p;  miR-483-5p;  分子标志物
分类号:
R735.1; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.02.002
文献标志码:
A
摘要:
目的 探讨miR-193a-3p,miR-337-5p和miR-483-5p在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)患者血清中的表达水平,评估其临床应用价值。方法 收集2011年6月～2014年5月南京军区南京总医院及徐州市肿瘤医院住院的63例ESCC患者术前、术后血清及随访信息,同时收集63例年龄、性别相匹配的健康体检者血清作为正常对照。利用TaqMan低密度芯片方法筛选出ESCC患者中异常表达的miRNA; 实时荧光定量PCR法验证芯片检测到的ESCC中明显上调的miR-193a-3p,miR-337-5p以及文献报道在口腔鳞状细胞癌(OSCC)表达上调的miR-483-5p; 最后评价它们的诊断和预后价值。结果 与健康对照相比,ESCC患者血清miR-193a-3p(0.459±0.339 vs 0.195±0.084),miR-337-5p(5.686±5.211 vs 2.476±0.808)和miR-483-5p(32.545±22.479 vs 19.509±10.601)的相对含量均显著升高(U值分别为591,605和1037,P值均<0.0001),且在患者术后明显降低(P值均<0.05); 三种miRNA的ROC AUC分别为0.851,0.848和0.739,三种miRNA组合在一起的AUC为0.873,均明显大于癌胚抗原; 术后高表达miR-483-5p的患者较之低表达的生存期短(P=0.022)。结论 血清miR-193a-3p,miR-337-5p和miR-483-5p可作为ESCC患者诊断或预后评估的潜在分子标志物。
Abstract:
Objective To investigate the expression level of serum miR-193a-3p,miR-337-5p and miR-483-5p in esophageal squamous cell carcinoma(ESCC)patients and to explore their value for diagnosis and prognosis of ESCC.Methods Serum samples were collected from 63ESCC patients before and after surgery in Nanjing General Hospital and Xuzhou Cancer Hospital between June 2013 and May 2014 and serum samples of 63 age- and sex- matched healthy individuals acted as the normal controls.TaqMan Low Density Assay was used to detect the deregulated miRNA in ESCC patients and then quantitative real-time PCR was used to validate the upregulated miRNA miR-193a-3p,miR-337-5p and previously reported miR-483-5p that was upregulated in oralsquamous cell carcinoma(OSCC).Finally,the three miRNA were evaluated for their clinical value in the diagnosis and predicting prognosis of ESCC.Results Compared with normal controls,serum levels of miR-193a-3p,miR-337-5p and miR-483-5p in ESCC patients were significantly up-regulated(0.459±0.339 vs 0.195±0.084,U=591; 5.686 ±5.211 vs 2.476±0.808,U=605; 32.545±22.479 vs 19.509±10.601,U=1 037,respectively,all P<0.0001)and their levels were significantly reduced after the surgical treatment(P<0.05).The areas under the ROC curve of serum miR-193a-3p,miR-337-5p,miR-483-5p and miR-Panel were all larger than that of CEA.Univariate Kaplan-Meier analysis revealed that ESCC patients with low expression level of miR-483-5p in postoperative serum exhibited higher survival rate than those with high level(P=0.022).Conclusion SerummiR-193a-3p,miR-337-5p and miR-483-5p can be potential molecular biomarkers in the diagnosis and predicting prognosis of ESCC.

参考文献/References:

[1] Torre LA,Bray F,Siegel RL,et al.Global cancer statistics,2012[J].CA Cancer J Clin,2015,65(2):87-108.
[2] Chen X,Ba Y,Ma L,et al.Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases[J].Cell Res,2008,18(10):997-1006.
[3] Zhang C,Wang C,Chen X,et al.Expression profile of microRNAs in serum: A fingerprint for esophageal squamous cell carcinoma[J].Clin Chem,2010,56(12):1871-1879.
[4] Liu R,Zhang C,Hu Z,et al.A five-microRNA signature identifies from genome-wide serum microRNA expression profiling serves as a fingerprint for gastric cancer diagnosis[J].Eur J Cancer,2011,47(5):784-791.
[5] Chen X,Liang H,Guan D,et al.A combination of let-7d,let-7g and let-7i serves as a stable reference for normalization of serum microRNAs[J].PLoS One,2013,8(11):e79652.
[6] Xu H,Yang Y,Zhao H,et al.Serum miR-483-5p:a novel diagnostic and prognostic biomarker for patients with oral squamous cell carcinoma[J].TumourBiol,2016,37(1):447-453.
[7] Xu X,Chen Z,Zhao X,et al.MicroRNA-25 promotes cell migration and invasion in esophageal squamous cell carcinoma[J].Biochem Biophys Res Commun,2012,421(4):640-645.
[8] Meng F,Qian L,Lv L,et al.MiR-193a-3p regulation of chemoradiation resistance in oesophageal cancer cells via the PSEN1 gene[J].Gene,2016,579(2):139-145.
[9] 唐海霞,王 成,路美玲,等.肾透明细胞癌患者血清miR-193a-3p水平增加及早期诊断价值[J].现代检验医学杂志,2015,30(3):43-45,49. Tang HX,Wang C,Lu ML,et al.Elevated serum miR-193a-3p level inrenal clear cell carcinoma and its early clinical value[J].Journal of ModernLaboratory Medicine,2015,30(3):43-45,49.
[10] Hamdi K,Goerlitz D,Stambouli N,et al.miRNAs in sera of tunisian patients discriminate between inflammatory breast cancer and non-inflammatory breast cancer[J].Springerplus,2014(3):636.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81472021,81672102)。
作者简介:贾亚旭(1991-),女,医学硕士研究生,主要研究方向:临床检验诊断学,E-mail:278646605@qq.com。
通讯作者:张春妮(1963-),女,博士,主任技师,博士生导师,主要研究方向:临床检验诊断学,E-mail: zchunni27@hotmail.com。
更新日期/Last Update: 2017-04-10