[1]刘贵生a,李文生b,郭雪艳a,等.不同亚型肠上皮化生和胃癌中CDX2蛋白的表达及其与幽门螺杆菌感染的关系[J].现代检验医学杂志,2017,32(02):10-14.[doi:10.3969/j.issn.1671-7414.2017.02.003]
　LIU Gui-shenga,LI Wen-shengb,GUO Xue-yana,et al.Expression of CDX2 and Its Relationship with Helicobacter Pylori Infection in Different Subtypes of Intestinal Metaplasia and Gastric Cancer[J].Journal of Modern Laboratory Medicine,2017,32(02):10-14.[doi:10.3969/j.issn.1671-7414.2017.02.003]
点击复制

不同亚型肠上皮化生和胃癌中CDX2蛋白的表达及其与幽门螺杆菌感染的关系()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年02期
页码:
10-14
栏目:
论著
出版日期:
2017-03-25

文章信息/Info

Title:
Expression of CDX2 and Its Relationship with Helicobacter Pylori Infection in Different Subtypes of Intestinal Metaplasia and Gastric Cancer
文章编号:
1671-7414(2017)02-010-05
作者:
刘贵生a; 李文生b; 郭雪艳a; 宗 伟a; 齐宗利c
陕西省人民医院a.消化内科; b.病理科; c.中心实验室,西安 710068
Author(s):
LIU Gui-shenga; LI Wen-shengb; GUO Xue-yana; ZONG Weia; QI Zong-lic
a.Department of Gastroenterology; b.Department of Pathology; c.Central Laboratory,Shaanxi Provincal People's Hospital,Xi'an 710068,China
关键词:
肠上皮化生;  幽门螺杆菌;  尾型同源异型盒基因2
分类号:
R735.2; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.02.003
文献标志码:
A
摘要:
目的 探讨不同亚型肠上皮化生(intestinal metaplasia,IM)中尾型同源异型盒基因2(CDX2)蛋白的表达及其与幽门螺杆菌(H.pylori)感染的关系。方法 应用免疫组化检测42例慢性萎缩性胃炎(CAG),46例CAG伴IM,34例癌旁组织伴IM,50例胃癌中CDX2蛋白的表达,应用HID-AB染色将80例IM分为Ⅰ型27例,Ⅱ型23例,Ⅲ型30例。应用HE染色、快速尿素酶试验和血清H.pylori-IgG抗体联合检测H.pylori感染,将80例IM分为H.pylori感染阳性组46例和H.pylori感染阴性组34例。结果 Ⅰ型、Ⅱ型、Ⅲ型IM各组中H.pylori阳性率分别为66.67%,65.22%和43.34%,各组间比较差异无统计学意义(χ2=3.953,P>0.05),CDX2蛋白表达阳性率分别为85.19%,69.57%和36.67%,Ⅲ型IM组显著低于Ⅰ型、Ⅱ型IM组(χ2=13.899,P<0.001和χ2=5.638,P=0.018),Ⅰ型、Ⅱ型IM之间差异无统计学意义(χ2=1.766,P=0.184)。各型IM组与胃癌组比较,Ⅰ型、Ⅱ型IM组CDX2表达阳性率显著高于胃癌组(χ2=14.517,P<0.001和χ2=5.509,P<0.05),而Ⅲ型IM组与胃癌组差异无统计学意义(χ2=0.088,P>0.05)。80例IM中H.pylori阳性组CDX2表达阳性率显著高于H.pylori阴性组(76.09% vs 44.12%,χ2=8.525,P=0.004)。不同亚型IM间比较,在Ⅰ型、Ⅱ型IM中,不同H.pylori感染情况下CDX2表达阳性率差异无统计学意义,但Ⅲ型IM中H.pylori阳性组CDX2表达阳性率显著高于H.pylori阴性组(P=0.023)。胃癌组中不同H.pylori感染情况下CDX2表达阳性率差异无统计学意义。结论 H.pylori感染可能通过影响不同亚型IM中CDX2的表达,进而导致IM的进展及胃癌的发生。
Abstract:
Objective To investigate the expression of CDX2in different subtypes of intestinal metaplasia(IM)and gastric cancer,and itsrelationship with Helicobacter Pylori(H.pylori)infection.Methods The expressions of CDX2 protein were detected with immunohistochemical method in 42 cases of chronic atrophic gastritis(CAG),46 cases ofCAG with IM,34 cases of paracancerous IM and 50 cases of gastric cancer.The IM was divided into three subtypes by HID-AB staining:27 cases of IM Ⅰ,23 cases of IM Ⅱ,and 30 cases of IM Ⅲ.H.pylori infection was detected with one minute rapid urease test,serum H.pylori IgG of ELISA method and HE staining in 80 caese of IM,which were divided into 46 cases of H.pylori-positive groups and 34 cases of H.pylori-negative groups.Results The positive rates of H.pylori infection in IM Ⅰ,IM Ⅱ and IM Ⅲwere 66.67%,65.22% and 43.34%,respectively,and there was no significant difference among different subtypes of IM(χ2=3.953,P>0.05).The positive rates of CDX2 expression in IM Ⅰ,IM Ⅱ and IM Ⅲ were 85.19%,69.57%and 36.67%,respectively,and IM Ⅲ were significant lower than IM Ⅰ,IM Ⅱ(χ2=13.899,P<0.001; χ2=5.638,P=0.018),and there wasno significant difference between IM Ⅰ and IM Ⅱ.Comparing of different types of IM group and gastric cancer group showed that the positive rates of CDX2 expression in IM Ⅰ,IM Ⅱ were significant higher than in gastric cancer group(χ2=14.517,P<0.001; χ2=5.509,P<0.05),but there was no significant difference between IM Ⅲ and gastric cancer group(χ2=0.088,P>0.05).The positive rates of CDX2 expression in H.pylori-positive groups was significant higher than in H.pylori-negative groups in all of IM(76.09% vs 44.12%,χ2=8.525,P=0.004).Comparing between different subtypes of IM showed that the positive rates of CDX2 expression in H.pylori-positive groups was significant higher than in H.pylori-negative groups in IM Ⅲ(P=0.023),but there was no significant difference between IM Ⅰ and IM Ⅱ(P>0.05).There was also no significant difference ofCDX2 expression between H.pylori-positive groups and H.pylori-negative groups in gastric cancer.Conclusion H.pylori infection may affect the progression of IM and gastric carcinogenesis by affecting the expression of CDX2 in different subtypes of IM.

参考文献/References:

[1] Bornschein J, Malfertheiner P.Helicobacter pylori and gastric cancer[J].Dig Dis,2014,32(3):249-264.
[2] Jiang JX,Liu Q,Zhao B,et al.Risk factors for intestinal metaplasia ina southeastern Chinese population:an analysis of 28,745 cases[J].J CancerRes Clin Oncol,2016 Nov 10,[Epub ahead of print].
[3] Sánchez Cuén JA,Irineo Cabrales AB,Bernal Magana G,et al.Regressionof gastric intestinal metaplasia after the eradication of Helicobacter pylori infection in a hospital in Mexico[J].Rev Esp Enferm Dig,2016,108(12):770-775.
[4] 刘贵生,龚 均,程 鹏,等.肠特异性转录因子C-DX2在不同亚型肠化生及胃癌组织中的表达[J].癌症,2006,25(2):185-189. Liu GS,Gong J,Cheng P,et al.Expression of intestine-specific transcription factor CDX2 in different subtypes of intestinal metaplasia and gastric carcinoma[J].Chinese Journal of Cancer,2006,25(2):185-189.
[5] 张 斌,时永全.胃黏膜肠化生调控分子的研究进展[J].中华消化病与影像杂志(电子版),2013,3(3):157-161. Zhang B,Shi YQ.Research progress of regulatory molecules involvedin intestinal metaplasia of gastric mucosa[J].Chin J Digest Med Imageol(Electronic Edition),2013,3(3):157-161.
[6] Haziri A,Juniku-Shkololli A,Gashi Z,et al.Helico-bacter pyloriinfection and precancerous lesions of the stomach[J].Med Arh,2010,64(4):248-249.
[7] Correa P,Piazuelo MB.The gastric precancerous cascade[J].J Dig Dis,2012,13(1):2-9.
[8] 闫春晓,张翠萍,戴素美.幽门螺杆菌感染与慢性胃炎相关性[J].齐鲁医学杂志,2012,27(1):40-42. Yan CX,Zhang CP,Dai SM.Correlation between Helicobacter pylori and chronic gastritis[J].Medical Journal of Qilu,2012,27(1):40-42.
[9] 高 光,金仁顺.SP和CDX2表达的胃黏膜上皮化生与胃癌的关系研究[J].肿瘤学杂志,2016,22(4):291-295. Gao G,Jin RS.Relationship between SP and CDX2 expressed gastric metaplasia and gastric carcinoma[J].Journal of Chinese Oncology,2016,22(4):291-295.
[10] Shiotani A,Nishi R,Uedo N,et al.Helicobacter pylori eradication prevents extension of intestinalization even in the high-risk group forgastric cancer[J].Digestion,2010,81(4):223-230.
[11] Shin CM,Kim N,Chang H,et al.Follow-Up study on CDX1 and CDX2 mRNA expression in noncancerous gastric mucosae after Helicobacter pylori eradication[J].Dig Dis Sci,2016,61(4):1051-1059.
[12] 王旭光,张 忠,张 晔,等.不同类型胃黏膜肠上皮化生CDX2蛋白的表达及幽门螺杆菌感染对其表达的影响[J].中国医科大学学报,2008,37(6):755-757. Wang XG,Zhang Z,Zhang Y,et al.Expression of CDX2 and its relationshipwith Helicobacter Pylori in different types of intestinal metaplasia of gastric mucosa[J].Journal of China Medical University,2008,37(6):755-757.
[13] 肖法嫚,孙 嫣,田 华,等.CDX2蛋白在不同亚型肠上皮化生中的表达及其与H.pylori感染的关系[J].胃肠病学和肝病学杂志,2009,18(6):550-552. Xiao FM,Sun Y,Tian H,et al.Expression of CDX2 in different subtypes ofintestinal metaplasia and its relation to H.pylori infection[J].Chin JGastroenterol Hepatol,2009,18(6):550-552.
[14] Zhang Y,Wang H,Bi C,et al.Expression of CDX2 in gastric cardia adenocarcinoma and its correlation with H.pylori and cell proliferation[J].Oncotarget,2016,7(34):54973-54982.
[15] Pilon N,Oh K,Sylvestre JR,et al.Wnt signaling is a key mediator of CDX1 expression in vivo[J].Development,2007,134(12):2315-2323.

相似文献/References:

[1]胡 娟,林 薇,呼永河,等.幽门螺杆菌在不同湿度环境下生活人群感染差异性的研究[J].现代检验医学杂志,2015,30(04):96.[doi:10.3969/j.issn.1671-7414.2015.04.027]
　HU Juan,LIN Wei,HU Yong-he,et al.Differences of Helicobacter Pylori Infection between Residents in Different Humidity Environment[J].Journal of Modern Laboratory Medicine,2015,30(02):96.[doi:10.3969/j.issn.1671-7414.2015.04.027]
[2]朱卫华,刘继斌,林兰.胃癌相关甲基化分型与幽门螺杆菌感染的探讨[J].现代检验医学杂志,2016,31(06):55.[doi:10.3969/j.issn.1671-7414.2016.06.015]
　ZHU Wei-hua,LIU Ji-bin,LIN Lan.Relationship between the CpG Island Methylator Phenotype and Helicobacter Pylori Infection Associated with Gastric Cancer[J].Journal of Modern Laboratory Medicine,2016,31(02):55.[doi:10.3969/j.issn.1671-7414.2016.06.015]
[3]杜 洁a,朱 娜b,李红艳a,等.幽门螺杆菌感染对慢性阻塞性肺疾病血气分析及肺功能的影响[J].现代检验医学杂志,2017,32(06):99.[doi:10.3969/j.issn.1671-7414.2017.06.001]
　DU Jiea,ZHU Nab,LI Hong-yana,et al.Effect of Helicobacter Pylori Infection on Blood Gas Analysis and Pulmonary Function in Patients with Chronic Obstructive Pulmonary Disease[J].Journal of Modern Laboratory Medicine,2017,32(02):99.[doi:10.3969/j.issn.1671-7414.2017.06.001]
[4]田文静,刘电力,刘惠敏,等.人血清幽门螺杆菌SodB抗体检测与胃癌发生的相关性研究[J].现代检验医学杂志,2018,33(01):22.[doi:10.3969/j.issn.1671-7414.2018.01.001]
　TIAN Wen-jing,LIU Dian-li,LIU Hui-min,et al.Correlative Study between Detection of Human Serum Helicobacter Pylori SodB Antibody and the Occurrence of Gastric Cancer[J].Journal of Modern Laboratory Medicine,2018,33(02):22.[doi:10.3969/j.issn.1671-7414.2018.01.001]
[5]陈立勇,张 静,陈尊义,等.慢性荨麻疹与幽门螺杆菌感染相关性探究[J].现代检验医学杂志,2018,33(04):146.[doi:10.3969/j.issn.1671-7414.2018.04.041]
　CHEN Li-yong,ZHANG Jing,CHEN Zun-yi,et al.Investigation of the Correlation between Chronic Urticaria and Helicobacter Pylori Infection[J].Journal of Modern Laboratory Medicine,2018,33(02):146.[doi:10.3969/j.issn.1671-7414.2018.04.041]
[6]高建华,何颖辉.冠心病并发幽门螺杆菌感染患者血清HP抗体谱特征与炎性因子水平相关性分析[J].现代检验医学杂志,2019,34(04):53.[doi:10.3969/j.issn.1671-7414.2019.04.013]
　GAO Jian-hua,HE Ying-hui.Correlation Analysis of Serum Antibody Spectrum and Inflammatory Factor Level in Patients with Coronary Heart Disease Complicated with Helicobacter Polori Infection[J].Journal of Modern Laboratory Medicine,2019,34(02):53.[doi:10.3969/j.issn.1671-7414.2019.04.013]

备注/Memo

备注/Memo:
基金项目:陕西省自然科学基金资助项目(重点项目:No2012JZ4003)。
作者简介:刘贵生(1969-),男,博士,硕士生导师,研究方向:胃癌及其癌前病变发病机制,E-mail:liuguish@126.com。
更新日期/Last Update: 2017-04-10