[1]吴 奇,申高飞,孙丽娜,等.miR-181d在结肠癌中表达失调并抑制癌细胞增殖及凋亡[J].现代检验医学杂志,2017,32(02):15-18，22.[doi:10.3969/j.issn.1671-7414.2017.02.004]
　WU Qi,SHEN Gao-fei,SUN Li-na,et al.miR-181d Inhibits Proliferation and Promotes Apoptosis in Colon Cancer Cells[J].Journal of Modern Laboratory Medicine,2017,32(02):15-18，22.[doi:10.3969/j.issn.1671-7414.2017.02.004]
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miR-181d在结肠癌中表达失调并抑制癌细胞增殖及凋亡()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年02期
页码:
15-18，22
栏目:
论著
出版日期:
2017-03-25

文章信息/Info

Title:
miR-181d Inhibits Proliferation and Promotes Apoptosis in Colon Cancer Cells
文章编号:
1671-7414(2017)02-015-05
作者:
吴 奇1; 申高飞2; 孙丽娜2; 汪 鑫2; 耿 蕾2; 杜 风2; 赵晓迪2; 卢瑗瑗2
1.第四军医大学学员旅,西安 710032;
2.第四军医大学西京消化病医院 肿瘤生物学国家重点实验室,西安 710032
Author(s):
WU Qi1; SHEN Gao-fei2; SUN Li-na2; WANG Xin2; GENG Lei2; DU Feng2; ZHAO Xiao-di2;  LU Yuan-yuan2
1.Student Brigade,the Fourth Military Medical University, Xi'an 710032,China;
2.State Key Laboratory of Cancer Biology,Xijing Hospital of Digestive Diseases,the Fourth Military Medical University,Xi'an 710032,China
关键词:
结肠癌;  miR-181d;  增殖;  凋亡
分类号:
R735.35; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.02.004
文献标志码:
A
摘要:
目的 检测结肠癌细胞系中miR-181d的表达水平,研究miR-181d对结肠癌细胞增殖及凋亡的影响。方法 RT-qPCR检测结肠癌细胞HCT116,HT29,LoVo,SW480及SW620和永生化肠黏膜上皮细胞HIEC中miR-181d的表达。在LoVo细胞中转染miR-181d模拟物及对照,SW620细胞中转染miR-181d抑制物及对照,RT-qPCR检测转染效率,MTT法检测细胞增殖,流式细胞术检测细胞周期和细胞凋亡比例。结果 miR-181d表达水平在各结肠癌细胞系中均较正常肠黏膜上皮细胞HIEC显著降低(F=29.34,P<0.01)。在LoVo细胞中过表达miR-181d能够显著减慢细胞体外增殖(F=5.403,P<0.01),细胞周期检测示S期细胞比例降低(t=4.71,P<0.05),凋亡细胞显著增多(t=3.47,P<0.05)。在SW620细胞中抑制miR-181d的表达能够显著加快细胞体外增殖(F=20.82,P<0.01),细胞周期检测示细胞周期加速,S期细胞比例增高(t=2.92,P<0.05),细胞凋亡率显著减少(t=4.14,P<0.05)。结论 miR-181d在结肠癌细胞系中表达普遍下调,有望成为结肠癌新的诊断标志物。miR-181d能够通过抑制结肠癌细胞增殖和促进凋亡,发挥抑癌基因的作用。
Abstract:
Objective To detect miR-181d expression levelsin colon cancer cell lines,and to study the functions of miR-181d on colon cancer cell proliferation and apoptosis.Methods RT-qPCRwas employed to study miR-181d expression levels in colon cancer cell line HCT116,HT29,LoVo,SW480 and SW620 cells,as well as in colon normal epithelial cell line HIEC.miR-181d mimic and control were transfected into LoVo cells whilemiR-181d inhibitor and control were transfected into SW620 cells.qRT-PCR wasperformed to validate the transfection efficiency.MTT assay was performed to measure cell proliferation while flow cytometry was performed to detect cell cycleand apoptosis rate.Results miR-181d was universally downregulated in all colon cancer cell lines compared to the colon normal epithelial cell line HIEC(F=29.34,P<0.01).Overexpression of miR-181d in LoVo cells significantly decreased in vitro cell proliferation rate(F=5.403,P<0.01).Flow cytometry indicated that cells at S phase were greatly decreased(t=4.71,P<0.05)and apoptotic cells were greatly increased compared to the control cells(t=3.47,P<0.05).On the contrary,inhibition of miR-181d inSW620 cells significantly promoted cell proliferation(F=20.82,P<0.01).Cell cycle was accelerated with significant increase in S phase compared to thecontrol cells(t=2.92,P<0.05),whereas apoptosis ratio was significantlydecreased(t=4.14,P<0.05).Conclusion miR-181d was universally downregulated in colon cancer cell lines compared to the normal epithelial cell line.miR-181d inhibits cell proliferation and induces apoptosis,thus functions as an tumor suppressive miRNA.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金面上项目(81572929,81602641)。
作者简介:吴 奇(1995-),男,在读本科生,E-mail:wcswq@qq.com。
通讯作者:卢瑗瑗(1982-),女,主治医师,讲师,研究方向:消化道肿瘤的分子诊断及治疗靶点研究,E-mail:luyuandreamer@aliyun.com。
赵晓迪(1987-),主治医师,讲师,研究方向:消化系肿瘤发生发展的分子机制研究,E-mail:leedyzhao@aliyun.com。
更新日期/Last Update: 2017-04-10