[1]赵英伦,马 元,李东冉,等.凋亡促进因子TFAR19,Apaf-1与腰椎间盘突出症的关系研究[J].现代检验医学杂志,2017,32(02):30-32.[doi:10.3969/j.issn.1671-7414.2017.02.008]
　ZHAO Ying-lun,MA Yuan,LI Dong-ran,et al.Relationship between Apoptosis Promoting Effector Molecules TFAR19, Apaf-1 and Lubar Intervertebral Disc Protrusion[J].Journal of Modern Laboratory Medicine,2017,32(02):30-32.[doi:10.3969/j.issn.1671-7414.2017.02.008]
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凋亡促进因子TFAR19,Apaf-1与腰椎间盘突出症的关系研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年02期
页码:
30-32
栏目:
论著
出版日期:
2017-03-25

文章信息/Info

Title:
Relationship between Apoptosis Promoting Effector Molecules TFAR19, Apaf-1 and Lubar Intervertebral Disc Protrusion
文章编号:
1671-7414(2017)02-030-03
作者:
赵英伦1; 马 元1; 李东冉2; 王 军1; 刘 冲1; 詹新立1
1.广西医科大学第一附属医院脊柱骨病外科,南宁 530021;
2.广西壮族自治区人民医院骨科,南宁 530021
Author(s):
ZHAO Ying-lun1; MA Yuan1; LI Dong-ran2; WANG Jun1; LIU Chong1; ZHAN Xin-li1
1.Department of Spine and Osteopathy Ward,the First AffiliatedHospital of Guangxi Medical University,Nanning 530021,China;
2.Department of Osteopathy Ward, the People's Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,China
关键词:
腰椎间盘退行性病变;  腰椎间盘突出症;  TFAR19;  Apaf-1;  细胞凋亡
分类号:
R681.53; R392.11
DOI:
10.3969/j.issn.1671-7414.2017.02.008
文献标志码:
A
摘要:
目的 探究凋亡促进因子TFAR19,Apaf-1与腰椎间盘突出症关系。方法 随机选择广西医科大学第一附属医院脊柱骨病外科住院接受手术的腰椎间盘突出症患者99例,其中设单个节段突出的患者70例为A组,多个节段突出(腰椎间盘突出节段数量≥2个)的患者29例为B组,于入院次日收集外周静脉血。同时随机选取健康体检者40例作为对照组(C组)。采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测腰椎间盘突出症患者血清中TFAR19,Apaf-1浓度。结果 C组、B组及A组血清中TFAR19浓度分别为1.30±0.09,1.85±0.14和2.33±0.25 ng/ml,各组之间TFAR19浓度差异具有统计学意义(F=7.979,P<0.01)。与C组相比,A组(q=3.60,P=0.012)、B组(q=5.59,P<0.01)TFAR19浓度差异具有统计学意义。A组、B组之间TFAR19浓度差异有统计学意义(q=2.93,P=0.012)。C组、B组及A组血清中Apaf-1浓度分别为107.52±11.58, 159.22±11.87,203.20±20.21 pg/ml,各组之间Apaf-1浓度差异具有统计学意义(F=8.828,P<0.01)。与C组相比,A组(q=3.89,P=0.007)、B组(q=5.86,P<0.01)Apaf-1浓度差异具有统计学意义。A组、B组之间Apaf-1浓度差异有统计学意义(q=2.97,P=0.037)。各组间性别构成比差异无统计学意义(χ2=0.229,P=0.892)。各组之间年龄差异无统计学意义(F=0.091,P=0.91)。结论 腰椎间盘突出症患者血清TFAR19,Apaf-1表达上调加速了腰椎间盘退变过程,参与了腰椎间盘突出症的发生,并且与椎间盘突出节段数量相关,突出节段数量越多,TFAR19,Apaf-1表达量越高。
Abstract:
Objective To explore the relationship between apoptosis promoting effector molecules TFAR19,Apaf-1 and lubar intervertebral disc protrusion.Methods 99 of operation patients with lubar intervertebral disc protrusion in the First Affiliated Hospital of Guangxi Medical University Department of Spine and Osteoputhy were recruited.Among them,single segment of lubar intervertebral disc protrusion were 70(Group A),more than one segments of lubar intervertebral disc protrusion were 29(Group B).In addition,40 unrelated healthy people from physical examination center were enrolled as controls(Group C).Enzyme-linked immunosorbent assay(ELISA)was used to examine serum TFAR19,Apaf-1 levels in lubar intervertebral disc protrusion patients.Results The level of TFAR19 in Group A,Group Band Group C respectively were 1.85±0.14,2.33±0.25 and 1.30±0.09 ng/ml.The TFAR19 activity in each group was statistically significant difference(F=7.979,P<0.01).Compared with Group C,the TFAR19 activity in Group B(q=5.59,P<0.01),Group A(q=3.60,P=0.012)was statistically significant difference.The TFAR19 activity in lubar intervertebral disc protrusion patients subgroups(Group A,Group B)was statistically significant difference(q=2.93,P=0.012).The level of Apaf-1 in Group A,Group B and Group C respectively were 159.22±11.87,203.20±20.21 and 107.52±11.58 pg/ml.The Apaf-1 activity in each group was statistically significant difference(F=8.828,P<0.01).Compared with Group C,the Apaf-1 activity in Group B(q=5.86,P<0.01),Group A(q=3.89,P=0.007)was statistically significant difference.The Apaf-1 activity in lubar intervertebral disc protrusion patients subgroups(Group A,Group B)was statistically significant difference(q=2.97,P=0.037).Male/female ratio between each groups was not statistically significant difference(χ2=0.229,P=0.892).Age between each groups was not statistically significant difference(F=0.091,P=0.91).Conclusion The augment of TFAR19 and Apaf-1 promotes apoptosis of lubarintervertebral disc protrusion.It is connected with quantity of protrusive segments.The more segments of protrusion,the higher TFAR19 and Apaf-1 level of examination will be.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:本研究受国家自然科学基金(No.81560359),广西自然科学基金项目(NO.2012GXNSFAA053141,NO.2014GXNSFAA118173)资助。
作者简介:赵英伦(1992-),男,满族,在读硕士,研究方向:骨科,Tel:15078895040,E-mail:834904502@qq.com。
通讯作者:詹新立(1970-),男,E-mail:3cstar@163.com.
更新日期/Last Update: 2017-04-10