[1]任雪维,张吉才.肝细胞肝癌患者血浆miR-29a水平的变化及其临床意义[J].现代检验医学杂志,2017,32(02):33-35，40.[doi:10.3969/j.issn.1671-7414.2017.02.009]
　REN Xue-wei,ZHANG Ji-cai.Changes of Plasma miR-29a Levels in Patients with Hepatocellular Carcinoma and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2017,32(02):33-35，40.[doi:10.3969/j.issn.1671-7414.2017.02.009]
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肝细胞肝癌患者血浆miR-29a水平的变化及其临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年02期
页码:
33-35，40
栏目:
论著
出版日期:
2017-03-25

文章信息/Info

Title:
Changes of Plasma miR-29a Levels in Patients with Hepatocellular Carcinoma and Its Clinical Significance
文章编号:
1671-7414(2017)02-033-04
作者:
任雪维1; 张吉才1; 2
1.湖北医药学院,湖北十堰 442000;
2.湖北医药学院附属太和医院检验科,湖北十堰 442000
Author(s):
REN Xue-wei1; ZHANG Ji-cai1; 2
1.Hubei University of Medicine, Hubei Shiyan 442000,China;
2.Department of Clinical Laboratory, Taihe Hospital Affiliated to Hubei University of Medicine,Hubei Shiyan 442000,China
关键词:
miR-29a;  肝细胞肝癌;  肝硬化;  聚合酶链反应
分类号:
R735.7; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.02.009
文献标志码:
A
摘要:
目的 探讨肝细胞肝癌(hepatocellular carcinoma,HCC)患者血浆miR-29a水平的变化及其临床意义。方法 病例对照研究,收集湖北省十堰市太和医院2016年1月～6月期间入院的30例肝细胞肝癌HCC患者,30例肝硬化(cirrhosis of liver,LC)患者和30例健康对照(healthy controls,HC)的血浆标本,采用实时荧光定量PCR检测血浆中microRNA-29a(miR-29a)含量,通过受试者工作特征曲线(receiver operating characteristic curve,ROC)分析血浆miR-29a表达水平在肝癌诊断中的敏感度和特异度,并对HCC患者血浆中miR-29a含量和甲胎蛋白(AFP)含量进行相关性分析。结果 比较miR-29a的相对表达量,HCC组(3.38±8.37),LC组(8.79±3.80)和HC组(11.98±6.64)差异具有统计学意义(P=0.000),其中HCC组显著低于LC组(P=0.046)和HC组(P=0.001),LC组显著低于HC组(P=0.026)。ROC曲线下面积miR-29a为0.816(95% CI 0.695～0.938)小于AFP 0.918(95%CI 0.853～0.982),诊断效率miR-29a不如AFP。HCC患者血浆中miR-29a含量和AFP浓度具有显著相关性(P=0.002),血浆miR-29a和AFP联合应用有助于提高HCC的诊断效率。结论 HCC患者血浆miR-29a水平降低,可能成为HCC诊断的参考指标。
Abstract:
Objective To investigate the changes of plasmamiR-29a level in patients with hepatocellular carcinoma(HCC)and its clinical significance.Methods Case-control study,30 patients with HCC,30 patients with cirrhosis of liver(LC)and 30 healthy controls(HC)were recruited from Shiyan Taihe Hospital,2016 January toJune.The level of microRNA-29a(miR-29a)in plasma was detected by real-timequantitative PCR,and the sensitivity and specificity of plasma miR-29a expression in the diagnosis of HCC were analyzed by receiver operating characteristiccurve(ROC),and to analyze the correlation between the miR-29a and the alpha-fetoprotein(AFP)in patients with hepatocellular carcinoma,the combination ofmiR-29a and AFP could improve the diagnostic efficiency of HCC.Results The relative expression of miR-29a was significantly differentbetween HCC group(3.38±8.37),LC group(8.79±3.80)and HC group(11.98±6.64),the expression level of miR-29a in HCC group was significantly lower than that in LC group(P=0.046)and HC group(P=0.001),and that in LC group was significantly lower than that in HC group(P=0.026).The area under the ROC curve of miR-29a was 0.816(95% CI 0.695 to 0.938)less than AFP 0.918(95% CI 0.853 to 0.982),and the diagnostic efficiency of miR-29a was not as good as that of AFP.The levels of miR-29a and AFP in plasma of patientswith hepatocellular carcinoma were significantly correlated(P=0.002).Conclusion The level of miR-29a in plasma of patients withHCC decreased,which may become the reference index of HCC diagnosis.

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备注/Memo

备注/Memo:
基金项目:湖北省自然科学基金(2014CFB304)资助。
作者简介:任雪维(1991-),女,硕士在读,主要研究方向为肿瘤分子诊断,Tel:15727195809,E-mail:369385602@qq.com。
更新日期/Last Update: 2017-04-10